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1.5.1.15: methylenetetrahydrofolate dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about methylenetetrahydrofolate dehydrogenase (NAD+), go to the full flat file.

Word Map on EC 1.5.1.15

Reaction

5,10-methylenetetrahydrofolate
+
NAD+
=
5,10-methenyltetrahydrofolate
 +
NADH
 +
H+

Synonyms

5,10-CH2-THF dehydrogenase, 5,10-methylenetetrahydrofolate reductase, bifunctional N5,N10-methylene-H4F dehydrogenase/N5,N10-methenyltetrahydrofolate cyclohydrolase, FolD, methenyltetrahydrofolate cyclohydrolase, methylenetetrahydrofolate dehydrogenase (NAD+-dependent), methylenetetrahydrofolate dehydrogenase-cyclohydrolase, methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase, methylenetetrahydrofolate dehydrogenase/cyclohydrolase, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, methylenetetrahydrofolate reductase, methyleneTHF dehydrogenase, Mg2+-/phosphate-dependent dehydrogenase, Mg2+/NAD-dependent methylenetetrahydrofolate dehydrogenase, More, MSMEG_6596, MSMEG_6649, MTD, MTHFD2, MTHFD2L, MTHFR, MTHFR1, MTHFR2, NAD+-dependent methylene-H4F dehydrogenase, NAD-dependent dehydrogenase-cyclohydrolase, NADH-oxidizing methylenetetrahydrofolate reductase, NMDMC

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.15 methylenetetrahydrofolate dehydrogenase (NAD+)

Specific Activity

Specific Activity on EC 1.5.1.15 - methylenetetrahydrofolate dehydrogenase (NAD+)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0001
-
C-57/Bl, bone marrow and whole adrenal, CD-1, adrenal
0.0002
0.0003
0.0004
0.0005
0.0006
-
RLE cells, virus 3611.MSV v-raf oncogene-transformed
0.0007
-
thymus
0.00076
-
fibroblasts, syrian hamster embryo
0.0011
-
Swiss, bone marrow
0.0012
0.0014
-
adrenal medulla
0.0015
0.0017
-
cell line B-16 solid tumor
0.002
-
RLE cells, virus pRNR16 v-Ha-ras oncogene-transformed
0.0021
-
RLE cells, virus J2 v-raf/v-myc oncogene-transformed
0.0024
-
cell line M4, human cutaneous melanoma
0.0027
-
cell line MCF-7, 100% confluent
0.0028
-
cell line KB
0.003
-
cell line LR-73, chinese hamster ovary
0.0031
-
cell line K562, human chronic myeolcytic leukemia
0.0033
-
cell line TL3, EBV-transformed
0.0034
-
bone marrow
0.0035
-
cell line MCF-7, 50% confluent
0.0039
0.004
-
cell line CCRF-CEM
0.0042
0.0045
-
cell line CHO
0.0046
-
cell line TL4, EBV-transformed
0.0047
-
cell line MG-63, human osteosarcoma
0.0049
-
cell line BeWo, human choriocarcinoma
0.005
0.0052
-
adrenal cortex
0.0055
0.0058
-
whole adrenal
0.0059
-
cell line P815, ascites in vivo
0.0063
0.0066
-
cell line EL4, ascites in vivo
0.0068
-
L-cells, murine fibroblast
0.0069
-
cell line MCF-7, human breast carcinoma
0.0071
-
cell line P-388 leukemia ip
0.0078
-
cell line CCRF-CEM, human leukemia
0.0089
-
Ehrlich ascites, ascites in vivo
0.0094
-
cell line EL4, murine T-cell lymphoma
0.0097
-
cell line YAC, murine lymphoma (Moloney virus-induced)
0.0105
-
cell line 3T3-SV40, SV40 transformed
0.02
-
Ehrlich ascites, murine mastocytoma
0.024
mutant D190E, cosubstrate NAD+, pH 7.3
0.156
mutant R198A, cosubstrate NADP+, pH 7.3
0.16
mutant D133A, cosubstrate NADP+, pH 7.3
0.17
mutant R198A, cosubstrate NAD+, pH 7.3
0.207
mutant R198S, cosubstrate NAD+, pH 7.3
0.392
mutant D133A, cosubstrate NAD+, pH 7.3
1 - 2
pH 6.0, 37°C, purified enzyme, recombinant
1.01
mutant D133S, cosubstrate NADP+, pH 7.3
1.24
mutant R198K, cosubstrate NADP+, pH 7.3
1.54
mutant R198S, cosubstrate NADP+, pH 7.3
12.6
mutant R198K, cosubstrate NAD+, pH 7.3
12.9
-
embryonal carcinoma
2 - 2.5
wild-type, cosubstrate NAD+, pH 7.3
2.7
-
fibroblast
2.92
wild-type, cosubstrate NADP+, pH 7.3
3.4
-
embryo fibroblast
3.64
mutant D190N, cosubstrate NAD+, pH 7.3
3.96
mutant D190N, cosubstrate NADP+, pH 7.3
3.99
mutant D133S, cosubstrate NAD+, pH 7.3
31.9
-
-
4.6
mutant D133N, cosubstrate NAD+, pH 7.3
4.7
-
osteosarcoma
40 - 44
-
-
5
pH 6.0, 37°C, extract from Escherichia coli cells carrying the expression vector
5.5
-
mastocytoma
6.9
-
breast carcinoma
7.8
-
leukemia
8.9
-
adenocarcinoma
9.4
-
T-cell lymphoma
9.7
-
lymphoma