1.5.1.15: methylenetetrahydrofolate dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about methylenetetrahydrofolate dehydrogenase (NAD+), go to the full flat file.

Reaction

Synonyms

5,10-CH2-THF dehydrogenase, 5,10-methylenetetrahydrofolate reductase, bifunctional N5,N10-methylene-H4F dehydrogenase/N5,N10-methenyltetrahydrofolate cyclohydrolase, FolD, methenyltetrahydrofolate cyclohydrolase, methylenetetrahydrofolate dehydrogenase (NAD+-dependent), methylenetetrahydrofolate dehydrogenase-cyclohydrolase, methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase, methylenetetrahydrofolate dehydrogenase/cyclohydrolase, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, methylenetetrahydrofolate reductase, methyleneTHF dehydrogenase, Mg2+-/phosphate-dependent dehydrogenase, Mg2+/NAD-dependent methylenetetrahydrofolate dehydrogenase, More, MSMEG_6596, MSMEG_6649, MTD, MTHFD2, MTHFD2L, MTHFR, MTHFR1, MTHFR2, NAD+-dependent methylene-H4F dehydrogenase, NAD-dependent dehydrogenase-cyclohydrolase, NADH-oxidizing methylenetetrahydrofolate reductase, NMDMC

ECTree

     1 Oxidoreductases

         1.5 Acting on the CH-NH group of donors

             1.5.1 With NAD⁺ or NADP⁺ as acceptor

                1.5.1.15 methylenetetrahydrofolate dehydrogenase (NAD+)

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Results	in table
9	Activating Compound
43	AA Sequence
6	Application
1	CAS Registry Number
21	Cloned(Commentary)
49	Cofactor
3	Crystallization (Commentary)
20595	Disease/ Diagnostics
1	Expression
10	General Information
1	General Stability
7	Inhibitors
13	kcat/KM [mM/s]
7	Ki Value [mM]
63	KM Value [mM]
59	Localization
21	Metals/Ions
28	Molecular Weight [Da]
149	Natural Substrates/ Products (Substrates)
100	Organism
1	Oxidation Stability
3	Pathway
23	PDB ID
4	pH Optimum
1	pH Range
1	pH Stability
24	Protein Variants
23	Purification (Commentary)
2	Reaction
3	Reaction Type
86	Reference
79	Source Tissue
101	Specific Activity [micromol/min/mg]
3	Storage Stability
183	Substrates and Products (Substrate)
28	Subunits
59	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Stability [°C]
21	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.5.1.15 - methylenetetrahydrofolate dehydrogenase (NAD+)

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

construction of homology model

Homo sapiens

P13995

674480

crystal structure of the enzyme (MTHFD2) in complex with a substrate-based inhibitor and the enzyme cofactors NAD+ and inorganic phosphate

Homo sapiens

P13995

756337

protein crystallized from 7% PEG 8000 in 50 mM Tris-HCl, pH 8.2 at 4°C in both hanging and sitting drop conditions, space group P4212 with cell constants a/b/c 75.9/75,9/160.0 A, crystals diffract to 2.9 A resolution, crystals form as tetragonal bipyramids and may grow as large as 0.45 mm on a side

Saccharomyces cerevisiae

-

392089