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1.5.1.15: methylenetetrahydrofolate dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about methylenetetrahydrofolate dehydrogenase (NAD+), go to the full flat file.

Word Map on EC 1.5.1.15

Reaction

5,10-methylenetetrahydrofolate
+
NAD+
=
5,10-methenyltetrahydrofolate
 +
NADH
 +
H+

Synonyms

5,10-CH2-THF dehydrogenase, 5,10-methylenetetrahydrofolate reductase, bifunctional N5,N10-methylene-H4F dehydrogenase/N5,N10-methenyltetrahydrofolate cyclohydrolase, FolD, methenyltetrahydrofolate cyclohydrolase, methylenetetrahydrofolate dehydrogenase (NAD+-dependent), methylenetetrahydrofolate dehydrogenase-cyclohydrolase, methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase, methylenetetrahydrofolate dehydrogenase/cyclohydrolase, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, methylenetetrahydrofolate reductase, methyleneTHF dehydrogenase, Mg2+-/phosphate-dependent dehydrogenase, Mg2+/NAD-dependent methylenetetrahydrofolate dehydrogenase, More, MSMEG_6596, MSMEG_6649, MTD, MTHFD2, MTHFD2L, MTHFR, MTHFR1, MTHFR2, NAD+-dependent methylene-H4F dehydrogenase, NAD-dependent dehydrogenase-cyclohydrolase, NADH-oxidizing methylenetetrahydrofolate reductase, NMDMC

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.15 methylenetetrahydrofolate dehydrogenase (NAD+)

Cloned

Cloned on EC 1.5.1.15 - methylenetetrahydrofolate dehydrogenase (NAD+)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
6His-tagged enzyme is expressed in Escherichia coli
cDNA clones of gene DNMDMC encoding the bifunctional protein
-
cloned cDNA for the human enzyme expressed in Escherichia coli K-38
-
DNA sequencing
-
expression in Escherichia coli BL21(DE3)
full-length cDNA clone encoding NAD-dependent dehydrogenase-cyclohydrolase
-
gene MSMEG_6596, DNA and amino acid sequence determination and analysis, sequence comparisons
gene MSMEG_6649, DNA and amino acid sequence determination and analysis, sequence comparisons
heterologously overproduced in Escherichia coli with a C-terminal His6-tag
isolation of cDNA and the gene encoding the murine cytoplasmic methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase (DCS)
-
recombinant enzyme, expressed in Escherichia coli
-
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain AB1909
-
yeast gene MTD1 cloned and sequenced
-