1.5.1.11: D-octopine dehydrogenase
This is an abbreviated version!
For detailed information about D-octopine dehydrogenase, go to the full flat file.
Reaction
Synonyms
D-Octopine synthase, Dehydrogenase, octopine, flavin-containing opine dehydrogenase, N2-(D-1-carboxyethyl)-L-arginine:NAD+ oxidoreductase, N2-(D-1-carboxyethyl)-L-arginine:NAD+-oxidoreductase, OcDH, OCS, Octopine dehydrogenase, Octopine synthase, Octopine:NAD oxidoreductase, ODH, pTi_008
ECTree
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Turnover Number
Turnover Number on EC 1.5.1.11 - D-octopine dehydrogenase
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526
2-oxobutanoate
wild type enzyme, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
272
2-oxovalerate
wild type enzyme, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
2 - 8
mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
5.1
L-Arg
mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
43
L-Arg
mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
120
L-Arg
mutant enzyme Q118A, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
122
L-Arg
mutant enzyme Q118D, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
444
L-Arg
mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
446
L-Arg
mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
640
L-Arg
wild type enzyme, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
640
L-Arg
wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
7.3
mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
9.3
NADH
mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
25
NADH
mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
47
NADH
mutant enzyme Q118D, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
173
NADH
mutant enzyme Q118A, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
410
NADH
mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
422
NADH
mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
652
NADH
wild type enzyme, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
652
NADH
wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
13
mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
15.4
pyruvate
mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
22.5
pyruvate
mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
73
pyruvate
mutant enzyme Q118D, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
283
pyruvate
mutant enzyme Q118A, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
499
pyruvate
mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
511
pyruvate
mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
775
pyruvate
wild type enzyme, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
775
pyruvate
wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C