Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.4.9.1: methylamine dehydrogenase (amicyanin)

This is an abbreviated version!
For detailed information about methylamine dehydrogenase (amicyanin), go to the full flat file.

Word Map on EC 1.4.9.1

Reaction

methylamine
+
H2O
+ 2 amicyanin =
formaldehyde
+
NH3
+ 2 reduced amicyanin

Synonyms

amine dehydrogenase, amine: oxidoreductase (acceptor deaminating), dehydrogenase, amine, EC 1.4.98.1, EC 1.4.99.3, Heme 2, MADH, mauA, methylamine dehydrogenase, primary-amine dehydrogenase, QH-AmDH, QHNDH, quinohaemoprotein amine dehydrogenase, quinohemoprotein amine dehydrogenase, quinohemoprotein amine dehydrogenases, sQH-AmDH

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.9 With a copper protein as acceptor
                1.4.9.1 methylamine dehydrogenase (amicyanin)

Molecular Weight

Molecular Weight on EC 1.4.9.1 - methylamine dehydrogenase (amicyanin)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
101700
-
calculated from amino acid sequence
112000
-
gel filtration
119000
12000
-
two bands appear in an SDS-PAGE: 12 kDa and 42 kDa
133000
-
ultracentrifugation
138000
-
complex of methylamine dehydrogenase and amicyanin, gel filtration
13960
-
L subunit, MALDI
13970
-
L subunit, calculated from sequence
14000
-
2 * 14000 + 2 * 41000, X-ray crystallography
14200
-
SDS-PAGE, beta subunit
36500
38500
Mycobacterium convolutum
-
gel filtration
39000
-
1 * 60000 + 1 * 39000, SDS-PAGE
39234
-
1 * 53917 + 1 * 39234 + 1 * 8597, calculated from amino acid sequence
39360
-
calculated for beta-subunit, containing a 26 aa sequence which seems to correspond to a signal peptide that might be also involved in the correct translocation from the bacterial cytoplasm to the periplasmic space.
41000
-
2 * 14000 + 2 * 41000, X-ray crystallography
41160
-
H subunit, calculated from sequence
41170
-
H subunit, MALDI
42000
43300
-
SDS-PAGE, alpha subunit
47000
-
1 * 47000, SDS-PAGE
53917
-
1 * 53917 + 1 * 39234 + 1 * 8597, calculated from amino acid sequence
54230
-
calculated for alpha-subunit, existence in this protein of two consensus sequences (CXXCH) identifying as binding pockets for the two haem groups
56000
-
gel filtration
58000
-
1 * 58000 + 1 * 42000, SDS-PAGE
59500
60000
-
1 * 60000 + 1 * 39000, SDS-PAGE
8486
-
small subunit, MALDI-TOF mass spectrometry
8579
-
calculated gamma-subunit
8597
-
1 * 53917 + 1 * 39234 + 1 * 8597, calculated from amino acid sequence
95300
-
sedimentation equilibrium