1.4.3.3: D-amino-acid oxidase
This is an abbreviated version!
For detailed information about D-amino-acid oxidase, go to the full flat file.
Word Map on EC 1.4.3.3
-
1.4.3.3
-
d-serine
-
schizophrenia
-
peroxisomal
-
flavin
-
n-methyl-d-aspartate
-
d-alanine
-
catalase
-
fad
-
nmda
-
deamination
-
benzoate
-
flavoenzyme
-
flavoproteins
-
racemase
-
variabilis
-
l-amino
-
neurotransmission
-
d-aspartate
-
gracilis
-
co-agonist
-
rhodotorula
-
cephalosporin
-
glutamatergic
-
urate
-
d-proline
-
d-ala
-
d-ser
-
imino
-
antipsychotic
-
hypofunction
-
d-methionine
-
isoalloxazine
-
acylase
-
fad-containing
-
toruloides
-
rhodosporidium
-
d-glutamate
-
fad-dependent
-
d-cysteine
-
kynurenic
-
sulfurtransferase
-
d-valine
-
synthesis
-
medicine
-
d-leucine
-
cerium
-
7-aminocephalosporanic
-
d-tryptophan
-
d-phenylalanine
-
neuregulin
-
3-mercaptopyruvate
-
sarcosine
-
industry
-
biotechnology
-
analysis
-
diagnostics
-
drug development
-
pharmacology
- 1.4.3.3
- d-serine
-
schizophrenia
- peroxisomal
- flavin
- n-methyl-d-aspartate
- d-alanine
- catalase
- fad
- nmda
-
deamination
- benzoate
-
flavoenzyme
- flavoproteins
- racemase
- variabilis
-
l-amino
-
neurotransmission
- d-aspartate
- gracilis
-
co-agonist
- rhodotorula
- cephalosporin
-
glutamatergic
- urate
- d-proline
- d-ala
- d-ser
-
imino
-
antipsychotic
-
hypofunction
- d-methionine
- isoalloxazine
- acylase
-
fad-containing
- toruloides
- rhodosporidium
- d-glutamate
-
fad-dependent
- d-cysteine
-
kynurenic
- sulfurtransferase
- d-valine
- synthesis
- medicine
- d-leucine
- cerium
-
7-aminocephalosporanic
- d-tryptophan
- d-phenylalanine
- neuregulin
- 3-mercaptopyruvate
- sarcosine
- industry
- biotechnology
- analysis
- diagnostics
- drug development
- pharmacology
Reaction
Synonyms
chDAO, D-AAO, D-amino acid oxidase, D-amino-acid-oxidase, D-aminoacid oxidase, DAAO, DAMOX, DAO, DAO1, DaoE, hDAAO, ophio-amino-acid oxidase, oxidase, D-amino acid, PEG-DAO, pkDAAO, RgDAAO, TvDAAO, TvDAO, LH99
ECTree
Advanced search results
KM Value
KM Value on EC 1.4.3.3 - D-amino-acid oxidase
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2.9
(R)-alpha-methylbenzylamine
mutant Y228L/R283G, pH 8, 25°C. The kcat/Km profile exhibits a maximum at pH 10
2.94
mutant I230A/R283G, 30°C, pH 8.0
-
2.96
(RS)-1-(4-chlorophenyl)-1-phenylmethanamine
mutant I230C/R283G, 30°C, pH 8.0
-
3.73
(RS)-1-(4-chlorophenyl)-1-phenylmethanamine
mutant I230F/R283G, 30°C, pH 8.0
-
1
cephalosporin C
mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
1
cephalosporin C
mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
1.6
cephalosporin C
recombinant wild-type enzyme, pH 7.5, 22°C
1.7
cephalosporin C
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
1.9
cephalosporin C
mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
1.9
cephalosporin C
mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
2.5
cephalosporin C
mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
2.8
cephalosporin C
mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
3.8
cephalosporin C
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
4.3
cephalosporin C
mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
5
cephalosporin C
-
immobilized enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25°C
5
cephalosporin C
wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
6
cephalosporin C
-
free enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25°C
6
cephalosporin C
-
immobilized enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25°C
8.7
cephalosporin C
mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
9
cephalosporin C
-
free enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25°C
13
cephalosporin C
mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
28
cephalosporin C
mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
0.03
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.04
D-1-naphthyl-alanine
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.05
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.33
D-1-naphthyl-glycine
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.13
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.4
D-2-chloro-phenylglycine
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.5
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.5
D-2-fluoro-phenylglycine
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.04
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.06
D-2-naphthyl-alanine
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.01
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.03
D-2-naphthyl-glycine
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.03
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.2
D-4-chloro-phenylglycine
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.6
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.7
D-4-fluoro-phenylglycine
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
1.6
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
2.1
D-4-hydroxy-phenylglycine
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
16.7
D-Ala
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
0.22
D-alanine
mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
0.4
D-alanine
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
0.4
D-alanine
mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
0.4
D-alanine
mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
0.5
D-alanine
mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
0.6
D-alanine
mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
0.6
D-alanine
-
mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5
0.7
D-alanine
mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
0.8
D-alanine
wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
0.9
D-alanine
-
free enzyme, in 100 mM potassium phosphate buffer at pH 8.0 and 25°C
1.1
D-alanine
mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
1.1
D-alanine
mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
1.3
D-alanine
mutant enzyme Q339N, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
1.6
D-alanine
mutant enzyme W243I, assay in presence of a large excess of free FAD (0.2 mM) in assay mixture
1.6
D-alanine
-
immobilized enzyme, in 100 mM potassium phosphate buffer at pH 8.0 and 25°C
2
D-alanine
-
free enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25°C
2
D-alanine
-
immobilized enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25°C
3
D-alanine
-
free enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25°C
4
D-alanine
-
immobilized enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25°C
4.7
D-alanine
pH 8.5, 15°C, mutant S19G/S120P/Q144R/K321M/A345V
4.9
D-alanine
-
mutant enzyme M213G, apparent value, at 25°C and pH 8.5
5.5
D-alanine
apparent value, reconstituted holoenzyme at pH 8.0 and 30°C
18
D-alanine
mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
18.5
D-alanine
-
mutant enzyme M213R, apparent value, at 25°C and pH 8.5
40
D-alanine
mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
1.3
mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
2 - 3
D-arginine
mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
2.5
D-arginine
mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
5.5
D-arginine
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
5.5
D-arginine
mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
6.7
D-arginine
mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
9.2
D-arginine
mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
9.7
D-arginine
mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
13
D-arginine
mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
18
D-arginine
wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
29
D-arginine
mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
22
D-Asn
-
mutant enzyme F258A, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
22.6
D-Asn
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
29
D-Asn
-
mutant enzyme F258Y, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
50
D-Asn
-
Km above 50 mM, mutant enzyme F258S, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
2
mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
5.2
D-Aspartate
mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
15
D-Aspartate
mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
15
D-Aspartate
mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
17
D-Aspartate
mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
18
D-Aspartate
wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
30
D-Aspartate
mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
33
D-Aspartate
mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
53
D-Aspartate
mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
61
D-Aspartate
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
72
D-Aspartate
mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
98
D-Aspartate
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
150
D-Aspartate
mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value
5.1
-
mutant enzyme M213G, apparent value, at 25°C and pH 8.5
21.1
D-glutamate
-
mutant enzyme M213R, apparent value, at 25°C and pH 8.5
76
D-glutamate
-
wild type enzyme, apparent value, at 25°C and pH 8.5
101
D-glutamate
-
mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5
2.5
-
mutant enzyme M213G, apparent value, at 25°C and pH 8.5
5.4
D-glutamine
-
mutant enzyme M213R, apparent value, at 25°C and pH 8.5
5.4
D-glutamine
-
wild type enzyme, apparent value, at 25°C and pH 8.5
6
D-glutamine
-
mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5
0.15
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.3
D-homo-phenylalanine
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.227
D-Leu
-
mutant enzyme F258S, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
0.78
D-Leu
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
1.19
D-Leu
-
mutant enzyme F258A, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
1.81
D-Leu
-
mutant enzyme F258Y, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
29.3
D-Lys
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
16.6
D-Lysine
-
mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5
0.46
D-Met
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
2.9
D-Met
-
mutant enzyme F258A, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
3.3
D-Met
-
mutant enzyme F258S, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
4.2
D-Met
-
mutant enzyme F258Y, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
0.3
D-methionine
-
mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5
0.6
D-methionine
-
wild type enzyme, apparent value, at 25°C and pH 8.5
0.8
D-methionine
-
mutant enzyme M213G, apparent value, at 25°C and pH 8.5
1.2
D-methionine
-
mutant enzyme M213R, apparent value, at 25°C and pH 8.5
0.068
D-Phe
-
mutant enzyme F258A, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
0.134
D-Phe
-
mutant enzyme F258S, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
0.37
D-Phe
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
1.68
D-Phe
-
mutant enzyme F258Y, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
0.2
D-phenylalanine
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.3
D-phenylalanine
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
3.5
D-phenylglycine
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
5.2
D-phenylglycine
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
36.6
D-Ser
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
11.1
D-Thr
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
0.45
D-Trp
-
mutant enzyme F258A, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
0.46
D-Trp
-
mutant enzyme F258S, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
0.49
D-Trp
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
2.7
D-Trp
-
mutant enzyme F258Y, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
0.087
-
mutant enzyme F258A, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
0.45
D-Tyr
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
0.84
D-Tyr
-
mutant enzyme F258S, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
14.4
D-Val
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
41
D-Val
-
mutant enzyme F258A, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
46
D-Val
-
mutant enzyme F258S, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
additional information
-
the dextran-conjugated enzyme shows decreased KM-values for D-amino acids
-
additional information
additional information
steady-state kinetics, reductive and oxidative half-reactions, overview
-
additional information
additional information
DAAO-lingand interaction kinetics, overview
-
additional information
additional information
-
DAAO-lingand interaction kinetics, overview
-
additional information
additional information
-
kinetics in comparison to the human enzyme, overview
-
additional information
additional information
reaction kinetics of oxidative and reductive half-reactions of wild-type and mutant enzymes, overview
-