1.4.3.22: diamine oxidase
This is an abbreviated version!
For detailed information about diamine oxidase, go to the full flat file.
Word Map on EC 1.4.3.22
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1.4.3.22
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polyamine
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oxidases
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monoamine
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mucosal
-
copper
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spermidine
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deamination
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1.4.3.6
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aminoguanidine
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benzylamine
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endotoxin
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ornithine
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semicarbazide-sensitive
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occludin
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d-lactate
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copper-containing
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cadaverine
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bowel
-
biogenic
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semicarbazide
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villus
-
ileum
-
d-lactic
-
jejunal
-
lysyl
-
tyramine
-
zonula
-
piglets
-
pao
-
claudin-1
-
methylamine
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n-methyltransferase
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pargyline
-
agmatine
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tryptamine
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renalase
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hansenula
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occludens-1
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postheparin
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phenylhydrazine
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lentil
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deprenyl
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beta-aminopropionitrile
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oxidase-like
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clorgyline
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beta-phenylethylamine
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phenylethylamine
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quinoproteins
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samdc
-
globiformis
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diagnostics
-
analysis
-
biotechnology
-
medicine
- 1.4.3.22
- polyamine
- oxidases
-
monoamine
- mucosal
- copper
- spermidine
-
deamination
-
1.4.3.6
- aminoguanidine
- benzylamine
- endotoxin
- ornithine
-
semicarbazide-sensitive
- occludin
- d-lactate
-
copper-containing
- cadaverine
- bowel
-
biogenic
- semicarbazide
- villus
- ileum
-
d-lactic
- jejunal
-
lysyl
- tyramine
-
zonula
-
piglets
- pao
-
claudin-1
- methylamine
- n-methyltransferase
- pargyline
- agmatine
- tryptamine
- renalase
- hansenula
-
occludens-1
-
postheparin
- phenylhydrazine
- lentil
- deprenyl
- beta-aminopropionitrile
-
oxidase-like
- clorgyline
- beta-phenylethylamine
- phenylethylamine
-
quinoproteins
- samdc
- globiformis
- diagnostics
- analysis
- biotechnology
- medicine
Reaction
Synonyms
ABP1, AGAO, Amiloride-binding protein, Amine oxidase, BSAO, Copper amine oxidase, DAO, DAO-1, Diamine oxidase, EC 1.4.3.6, ELAO, GPAO, hDAO, histaminase, histamine dehydrogenase, histamine oxidase, LCAO, LSAO, methylputrescine oxidase, More, MPO, N-methylputrescine oxidase, pea seedling amine oxidase, PKAO, PPLO, PSAO, pWGH15, rhDAO, vDAO
ECTree
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Specific Activity
Specific Activity on EC 1.4.3.22 - diamine oxidase
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0.00037
substrate beta-phenylethylamine, at high substrate concentration
0.0326
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partially purified enzyme, N-methylpurtrescine as substrate, pH 7.9, 30°C
additional information
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DAO activity is determined by measuring of H2O2 gneration, a product of the polyamine oxidation. Samples are homogenized in 0.1 M potassium phosphate buffer, pH 6.5. Homogenate is centrifuged and the supernatant is used for assay. Reaction mixture contains 2.5 ml of 0.1 M potassium phosphate, pH 6.5, 0.2 ml 4-aminoantipyrine/N,N-dimethylaniline reaction solutions, 0.1 ml of horseradish peroxidase (250 U/ml) and 0.2 ml of the enzyme extract. The reaction is initiated by the addition of 15 microL of putrescine. Salinity stress (50 mM NaCl) causes a significant increase in DAO activity in cultivar Changchun mici roots but only a slight increase in cultivar Jinchun no. 2 roots. When salinity stress is combined with exogenous spermidine treatment, DAO activity increases in cultivar Jinchun no. 2, but only slightly in cv. Changchun mici.
additional information
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19.6 units/mg, 1 unit is defined as the change in absorbance of 0.1 at 470 nm/min
additional information
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the crude extract has a specific activity of 9.1 units/mg, while the 215fold purified enzyme has a specific activity of 1962.47 units/mg. A change in absorbance at 555 nm of 0.01 per minute is considered as one unit of enzyme activity