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1.4.3.22: diamine oxidase

This is an abbreviated version!
For detailed information about diamine oxidase, go to the full flat file.

Word Map on EC 1.4.3.22

Reaction

histamine
+
H2O
+
O2
=
(imidazol-4-yl)acetaldehyde
+
NH3
+
H2O2

Synonyms

ABP1, AGAO, Amiloride-binding protein, Amine oxidase, BSAO, Copper amine oxidase, DAO, DAO-1, Diamine oxidase, EC 1.4.3.6, ELAO, GPAO, hDAO, histaminase, histamine dehydrogenase, histamine oxidase, LCAO, LSAO, methylputrescine oxidase, More, MPO, N-methylputrescine oxidase, pea seedling amine oxidase, PKAO, PPLO, PSAO, pWGH15, rhDAO, vDAO

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.22 diamine oxidase

Purification

Purification on EC 1.4.3.22 - diamine oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography on AH-Sepharose
affinity chromatography on cadaverine-Sepharose
-
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-100 gel filtration
-
ammonium sulfate, heat denaturation, DEAE-cellulose, hydroxyapatite, Sephacryl S-300 HR
CM-23-cellulose, hydroxyapatite, Sephacryl S 300-HR
-
heparin-Sepharose column chromatography, phenyl-Sepharose column chromatography, CIM-QA column chromatography, and Superdex 200 gel filtration
-
isolated in 98% purity
Ni-NTA column chromatography and Superdex 200 gel filtration
-
partially
-
phosphocellulose, methylglyoxalbis(guanylhydrazone)-Sepharose
-
recombinant enzyme
-
recombinant secreted hDAO from Drosophila S2 cells