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1.4.3.22: diamine oxidase

This is an abbreviated version!
For detailed information about diamine oxidase, go to the full flat file.

Word Map on EC 1.4.3.22

Reaction

histamine
+
H2O
+
O2
=
(imidazol-4-yl)acetaldehyde
+
NH3
+
H2O2

Synonyms

ABP1, AGAO, Amiloride-binding protein, Amine oxidase, BSAO, Copper amine oxidase, DAO, DAO-1, Diamine oxidase, EC 1.4.3.6, ELAO, GPAO, hDAO, histaminase, histamine dehydrogenase, histamine oxidase, LCAO, LSAO, methylputrescine oxidase, More, MPO, N-methylputrescine oxidase, pea seedling amine oxidase, PKAO, PPLO, PSAO, pWGH15, rhDAO, vDAO

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.22 diamine oxidase

General Stability

General Stability on EC 1.4.3.22 - diamine oxidase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
cholic acids and lipids protected the enzyme in the simulated intestinal fluids
-
enzyme does not withstand lyophilization
purified enzyme does not withstand lyophilization
-
the enzyme shows weak stability under simulated intestinal conditions with a half-life period of around 19 min
unstable at low ionic strenght, half-life of 8 d in 5 mM potassium phosphate, pH 7.0, at 4°C, 33 d if 100 mM NaCl is added, unstable if 0.01% n-octyl-beta-D-glucopyranoside or 0.1% Triton X-100 are added, loss of 25% activity after 5 d
-
unstable in dilute solution or in frozen state
-