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1.4.3.21: primary-amine oxidase

This is an abbreviated version!
For detailed information about primary-amine oxidase, go to the full flat file.

Word Map on EC 1.4.3.21

Reaction

RCH2NH2
+
H2O
 +
O2
=
RCHO
 +
NH3
 +
H2O2

Synonyms

AGAO, AMAO2, AMAO3, amine oxidase 1, amine oxidase, copper containing, AO1, AO2, AOC2, AOC3, BAO, benzylamine oxidase, bovine plasma amine oxidase, bovine serum amine oxidase, BPAO, BSAO, CAO, Copper amine oxidase, copper amine oxidase 1, copper amine oxidase 8, copper-containing amine oxidase, copper-containing AO, copper-containing monoamine oxidase, copper-dependent amine oxidase, copper/quinone containing amine oxidase, Cu/TPQ amine oxidase, CuAO, CuAO1, CuAO2, CuAO3, CuAO8, EC 1.4.3.6, ECAO, ELAO, GPAO, grass pea amine oxidase, Hansenula polymorpha amine oxidase, HPAO, hPAO-1, HPAO-2, lentil seedling amine oxidase, LSAO, MAO-N, More, OVAO, PAO, pea seedling amine oxidase, plasma amine oxidase, PrAO, primary amine oxidase, primary-amine:oxygen oxidoreductase, PSAO, quinone- and copper-containing amine oxidase, quinone-containing copper amine oxidase, quinone-dependent amine oxidase, quinoprotein copper-containing amine oxidase, RAO, sainfoin amine oxidase, semicarbazide-sensitive amine oxidase, semicarbazide-sensitive amine oxidase/vascular adhesion protein-1, SSAO, SSAO/VAP-1, TPQ-containing CuAO, tynA, tyramine oxidase, copper-requiring, VAP-1, vascular adhesion protein 1, vascular adhesion protein-1

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.21 primary-amine oxidase

Purification

Purification on EC 1.4.3.21 - primary-amine oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Hi-Prep 16 10 Q column chromatography, Mono Q column chromatography, and Superose 12 gel filtration
-
ammonium sulfate precipitation, Sepharose column chromatography, and Sephacryl S-200 gel filtration
DEAE cellulose column chromatography, DEAE-Sephadex gel filtration, and hydroxylapatite column chromatography
-
DEAE-Toyopearl column chromatography, and phenyl-Toyopearl column chromatography
-
native and recombinant enzyme
-
Ni-chelating affinity column chromatography
Ni-NTA column chromatography
nickel affinity column chromatography
Q sepharose column chromatography
Q-Sepharose column chromatography, and gel filtration
recombinant enzyme expressed in Escherichia coli
recombinant His-tagged wild-type and mutants enzymes from Escherichia coli strain Rosetta2(DE3), removal of the His tag
-
recombinant selenomethionine-labeled truncated mutant MAO-N-D5 from Escherichia coli strain B834(DE3) by nickel affinity chromatography
-