Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.4.3.2: L-amino-acid oxidase

This is an abbreviated version!
For detailed information about L-amino-acid oxidase, go to the full flat file.

Word Map on EC 1.4.3.2

Reaction

an L-amino acid
+
H2O
 +
O2
=
a 2-oxo carboxylate
 +
NH3
 +
H2O2

Synonyms

AAD, ACTX-6, ACTX-8, Akbu-LAAO, APIT, apotxin, aromatic L-amino acid oxidase, Balt-LAAO-I, bordonein-L, Bpir-LAOO-1, CC-LPOX, DAA, Dolabellanin, escapin, head kidney and gill, IL4I1, Il4i1 protein, ink toxin 1, Interleukin Four Induced Gene 1 protein, L-AAO, L-AAO/MOG, L-AAO1, L-amino acid oxidase, L-amino acid: O2 oxidoreductase, L-amino acid:O2 oxidoreductase, L-amino acid:O2 oxidoreductase (deaminating), L-aminooxidase, L-Aox, L-phenylalanine oxidase, LAAO, LAAO-1, LAAO-I, LAAO-II, LAAO1, LAAO4, LAAOI, LAAOII, LAO, LAO1, M-LAO, ophio-amino-acid oxidase, Pm1, RoLAAO, sarA, Sebastes schlegeli antibacterial protein, Sebastes schlegelii antibacterial protein, SSAP, TJ-LAO, toxophallin, TSV-LAO

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.2 L-amino-acid oxidase

Storage Stability

Storage Stability on EC 1.4.3.2 - L-amino-acid oxidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, 50 mM glycine/NaOH buffer, pH 8.6, 50% glycerol, stable for several months, withou glycerol inactivation
-20°C, 1 month, 93% residual activity
-20°C, 24 h, 85% loss of activity
-
-20°C, 4 months, no loss of activity
Bacillus carotarum
-
-20°C, loss of 80% of activity
-
-80°C, at least 3 years
-
0-3°C, dialyzed enzyme, 2 weeks, 10% loss of activity
-
0-5°C, crystalline suspension in water, pH 7.2, stable for several months
-
0°C, several weeks
25°C, stable for at least 24h
-
4°C, 1 month, 76% residual activity
4°C, 4 months, no loss of activity
Bacillus carotarum
-
4°C, 40% v/v glycerol, 3 months
-
4°C, 50 mM glycine/NaOH buffer, pH 8.6, stable for several months
4°C, 66 mM KCl, 10 mM 2-mercaptoethanol, 1 month, 5-10% loss of activity
-
4°C, pH 7.4, 20 mM Tris-HCl, 3 months
-
4°C, purified enzyme stable for weeks
-
4°C, several months
-
4°C, stable for at least 24h
-
LAAO is more stable when kept at -80°C, followed by 4°C, reactivation using sodium acetate buffer pH 5.0 for 30 min at 37°C leads to increased activity
no decrease in antimicrobial activity during storage for up to 5 months at room temperature