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1.4.3.2: L-amino-acid oxidase

This is an abbreviated version!
For detailed information about L-amino-acid oxidase, go to the full flat file.

Word Map on EC 1.4.3.2

Reaction

an L-amino acid
+
H2O
 +
O2
=
a 2-oxo carboxylate
 +
NH3
 +
H2O2

Synonyms

AAD, ACTX-6, ACTX-8, Akbu-LAAO, APIT, apotxin, aromatic L-amino acid oxidase, Balt-LAAO-I, bordonein-L, Bpir-LAOO-1, CC-LPOX, DAA, Dolabellanin, escapin, head kidney and gill, IL4I1, Il4i1 protein, ink toxin 1, Interleukin Four Induced Gene 1 protein, L-AAO, L-AAO/MOG, L-AAO1, L-amino acid oxidase, L-amino acid: O2 oxidoreductase, L-amino acid:O2 oxidoreductase, L-amino acid:O2 oxidoreductase (deaminating), L-aminooxidase, L-Aox, L-phenylalanine oxidase, LAAO, LAAO-1, LAAO-I, LAAO-II, LAAO1, LAAO4, LAAOI, LAAOII, LAO, LAO1, M-LAO, ophio-amino-acid oxidase, Pm1, RoLAAO, sarA, Sebastes schlegeli antibacterial protein, Sebastes schlegelii antibacterial protein, SSAP, TJ-LAO, toxophallin, TSV-LAO

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.2 L-amino-acid oxidase

Crystallization

Crystallization on EC 1.4.3.2 - L-amino-acid oxidase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with L-phenylalanine, using polyethylene glycol monomethyl ether 550 as precipitant agent, and zinc sulfate as an additive
A0A0S1LJ33
hanging drop vapor diffusion method, using 0.1 M sodium acetate trihydrate pH 4.6 and 25% (w/v) polyethylene glycol 1000
-
in complex with substrate L-phenylalanine
-
in presence of citrate and o-aminobenzoate, comparison with D-amino acid oxidases
-
hanging drop vapour-diffusion method
-
homology modeling of LAAO1 predicts metal coordination by residues Y241, H348, and D406
in complex with flavin adenine dinucleotide. The overall fold is similar to D-amino acid oxidases with an additional hydrophobic insertion module on protein surface. The hydrophobic module serves as an extra membrane-binding site. Residues Q99 and R315 may interact with the carboxyl group of substrate amino acids, residues Q278 and L317 may be important for substrate selectivity
structures of L-AAO/MOG complexed with L-Lys, L-ornithine, and L-Arg. Residue Asp238 is located at the ceiling of a long hydrophobic pocket and forms a strong interaction with the terminal, positively charged group of the substrates. In the ligand-free structure, there are two channels connecting the active site and solvent, and a short region located at the dimer interface is disordered. In the L-Lys complex structure, a loop region is displaced to plug the channels
sitting drop vapor diffusion method, using 8% (w/v) polyethylene glycol 4000 and 0.1 M Na-acetate (pH 4.6)
as HgCl2-derivative, native protein and in complex with L-alanine, L-phenylalanine or o-aminobenzoate
-
native isozyme LAAO I from venom, sitting drop vapour-diffusion method, mixing of 0.001 ml of protein solution with 10 mg/ml protein with 0.001 ml of a precipitant solution consisting of 16% w/v PEG 3350 and 0.04 M zinc acetate against 1 ml of reservoir solution at 20°C for 2 days, X-ray diffraction structure determination and analysis at 2.6 A resolution
-
purified native enzyme by sitting drop vapour diffusion method, using a protein concentration of 10 mg/ml, with 0.04 M zinc acetate and 16% w/v PEG 3350 as the precipitant, X-ray diffraction structure determination and analysis at 2.57 A resolution
-