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1.4.3.19: glycine oxidase

This is an abbreviated version!
For detailed information about glycine oxidase, go to the full flat file.

Word Map on EC 1.4.3.19

Reaction

2-iminoacetate
+
H2O
=
glyoxylate
+
NH3

Synonyms

BcGO, glycin oxidase, glycine:oxygen oxidoreductase (deaminating), GlyOX, GO, GOX, GoxA, GOXK, GoxR, LodA, PlGoxA, ThiO

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.19 glycine oxidase

Temperature Stability

Temperature Stability on EC 1.4.3.19 - glycine oxidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 50
the enzyme remains stable between 0 and 50°C after 6 h incubation
25
-
5 h, most stable between pH 7.5 and 8.5
34.7
melting temperature, mutant G51R/A54R, determined by following protein and flavin fluorescence
35
-
pH 7.0, 30 min, stable up to
41.3
melting temperature, mutant G51R, determined by following protein and flavin fluorescence
44.8
melting temperature, mutant A54R, determined by following protein and flavin fluorescence
45.3
melting temperature, mutant G51S/A54R, determined by following protein and flavin fluorescence
45.7
melting temperature, mutant G51S/A54R/H244A, determined by following protein and flavin fluorescence
46
-
Tm-value, after 30 min
48
melting temperature, apoprotein
50
-
pH 7.0, 30 min, about 75% loss of activity
57
melting temperature
60 - 80
at 60°C, the I15V mutant shows a similar profile than the wild type enzyme but with lower residual activity after 20 min, at 65°C the mutant goes through activation (160%) during the first 15 min, the activity then falls with a time-dependent trend and reaches a half-life value similar to the wild type enzyme, at higher temperatures (70-80°C) the activity decreases rapidly in both cases, but with half-life value higher for I15V mutant than for wild type enzyme
95
-
heat treatment of the protein at 95°C for 5 min results in the release of the FAD cofactor