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1.4.3.1: D-aspartate oxidase

This is an abbreviated version!
For detailed information about D-aspartate oxidase, go to the full flat file.

Word Map on EC 1.4.3.1

Reaction

D-Aspartate
+
H2O
+
O2
=
oxaloacetate
+
NH3
+
H2O2

Synonyms

aspartic oxidase, C47A10.5 gene product, C47Ap, ChDASPO, ChDDO, D-Asp oxidase, D-aspartic oxidase, D-AspO, DAO, DASOX, DASPO, DDO, DDO-1, DDO-2, DDO-3, DDO1, DDO3, F18E3.7a gene product, F18Ep

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.1 D-aspartate oxidase

Specific Activity

Specific Activity on EC 1.4.3.1 - D-aspartate oxidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00021
-
from kidney
0.00048
-
from liver
0.00053
-
from brain
0.00055
-
from liver
0.0006
-
from kidney
0.00062
-
from kidney
0.00076
-
from kidney
0.00119
0.00144
-
from liver
0.00233
-
from liver
0.0035
-
-
0.08
unpurified recombinant enzyme
0.09
pH 7.0, 20°C, purified recombinant mutant R243A, substrate D-Asp
0.1
wild type enzyme, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with D-glutamate as substrate
0.11
mutant enzyme S308Y, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with D-glutamate as substrate
0.12
with D-Pro as substrate, at pH 8.3 and 37°C
0.14
with D-His as substrate, at pH 8.3 and 37°C
0.16
with D-Asn as substrate, at pH 8.3 and 37°C
0.24
mutant enzyme S308A, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with D-glutamate as substrate
0.4
-
-
0.46
with D-Glu as substrate, at pH 8.3 and 37°C
0.47
with N-methyl-L-Asp as substrate, at pH 8.3 and 37°C
0.5
pH 7.0, 20°C, purified recombinant mutant R243K, substrate D-Glu
0.53
substrate D-glutamate, pH 8.3, 37°C
0.55
mutant enzyme S308G, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with D-glutamate as substrate
1
pH 7.0, 20°C, purified recombinant mutant R243E, substrate D-Glu
1.2
pH 7.0, 20°C, purified recombinant mutant R243D, substrate N-methyl-D-Asp
1.5
pH 7.0, 20°C, purified recombinant mutant R243K, substrate D-Asp
1.6
pH 7.0, 20°C, purified recombinant wild-type enzyme, substrate D-Glu
1.77
substrate D-glutamate, pH 8.3, 37°C
1.9
pH 7.0, 20°C, purified recombinant mutant R243E, substrate D-Asp
11.7
substrate D-aspartate, pH 8.3, 37°C
12.2
with D-Asp as substrate, at pH 8.3 and 37°C
169
-
60°C, pH 7.5
18.5
pH 7.0, 20°C, purified recombinant mutant R243D, substrate D-Glu
181
pH 7.0, 20°C, purified recombinant mutant R243D, substrate D-Asp
2.37
mutant enzyme S308Y, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with D-aspartate as substrate
2.4
-
ferricyanide as electron acceptor
2.54
mutant enzyme S308Y, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with N-methyl-D-aspartate as substrate
2.93
with D-Asn as substrate, at pH 8.3 and 37°C
25.3
-
-
29.1
-
-
3.17
with N-methyl-D-Glu as substrate, at pH 8.3 and 37°C
4.31
wild type enzyme, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with D-aspartate as substrate
4.32
wild type enzyme, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with N-methyl-D-aspartate as substrate
44
substrate D-aspartate, pH 8.3, 37°C
46.3
-
37°C, pH 7.5
5.55
mutant enzyme S308A, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with D-aspartate as substrate
5.92
mutant enzyme S308A, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with N-methyl-D-aspartate as substrate
51.7
substrate D-aspartate, pH 8.3, 37°C
62
recombinant enzyme after 775fold purification
65.3
substrate D-glutamate, pH 8.3, 37°C
7.82
with D-Glu as substrate, at pH 8.3 and 37°C
86
pH 7.0, 20°C, purified recombinant mutant R243M, substrate D-Asp
87.5
with D-Asp as substrate, at pH 8.3 and 37°C
9.2
pH 7.0, 20°C, purified recombinant wild-type enzyme, substrate N-methyl-D-Asp
9.24
mutant enzyme S308G, at 37°C in 40 mM sodium diphosphate buffer (pH 8.3) with D-aspartate as substrate
91.2
with N-methyl-D-Asp as substrate, at pH 8.3 and 37°C
93.7
pH 7.0, 20°C, purified recombinant wild-type enzyme, substrate D-Asp
additional information
mutant activities with D-Ala and D-Arg, overview