1.4.3.1: D-aspartate oxidase
This is an abbreviated version!
For detailed information about D-aspartate oxidase, go to the full flat file.
Word Map on EC 1.4.3.1
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1.4.3.1
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d-amino
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n-methyl-d-aspartate
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nmda
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d-serine
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d-glutamate
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octopus
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nmdar-dependent
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d-alanine
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prepulse
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humicola
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d-amino-acid
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d-ser
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d-glu
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drug development
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molecular biology
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medicine
- 1.4.3.1
-
d-amino
- n-methyl-d-aspartate
- nmda
- d-serine
- d-glutamate
- octopus
-
nmdar-dependent
- d-alanine
-
prepulse
- humicola
-
d-amino-acid
- d-ser
- d-glu
- drug development
- molecular biology
- medicine
Reaction
Synonyms
aspartic oxidase, C47A10.5 gene product, C47Ap, ChDASPO, ChDDO, D-Asp oxidase, D-aspartic oxidase, D-AspO, DAO, DASOX, DASPO, DDO, DDO-1, DDO-2, DDO-3, DDO1, DDO3, F18E3.7a gene product, F18Ep
ECTree
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Purification
Purification on EC 1.4.3.1 - D-aspartate oxidase
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expressed in Escherichia coli, mixture of 80-91% active FAD-containing and 9-20% inactive OH-FAD containing enzyme
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His GraviTrap column chromatography
recombinant DDO-1, DDO-2, and DDO-3 from Escherichia coli to homogeneity or near homogeneity
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recombinant enzyme from Escherichia coli
recombinant His-tagged wild-type and mutant DDOs from Escherichia coli strain Rosetta (DE3) by metal affinity chromatography and gel filtration
recombinant protein, purified to homogeneity from the soluble fraction by HiTrap chelating chromatography
SP-Toyopearl column chromatography, Q-Sepharose column chromatography, PPG-Toyopearl column chromatography, and SuperSW3000 gel filtration