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1.4.3.1: D-aspartate oxidase

This is an abbreviated version!
For detailed information about D-aspartate oxidase, go to the full flat file.

Word Map on EC 1.4.3.1

Reaction

D-Aspartate
+
H2O
+
O2
=
oxaloacetate
+
NH3
+
H2O2

Synonyms

aspartic oxidase, C47A10.5 gene product, C47Ap, ChDASPO, ChDDO, D-Asp oxidase, D-aspartic oxidase, D-AspO, DAO, DASOX, DASPO, DDO, DDO-1, DDO-2, DDO-3, DDO1, DDO3, F18E3.7a gene product, F18Ep

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.1 D-aspartate oxidase

Purification

Purification on EC 1.4.3.1 - D-aspartate oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli, mixture of 80-91% active FAD-containing and 9-20% inactive OH-FAD containing enzyme
-
His GraviTrap column chromatography
HiTrap Chelating HP column chromatography
-
recombinant
-
recombinant C47A10.5 gene product
-
recombinant chDDO, ammonium sulfate, Butyl-Sepharose, SP-Sepharose
recombinant DDO-1, DDO-2, and DDO-3 from Escherichia coli to homogeneity or near homogeneity
-
recombinant enzyme
-
recombinant enzyme from Escherichia coli
recombinant F18E3.7a gene product
-
recombinant His-tagged wild-type and mutant DDOs from Escherichia coli strain Rosetta (DE3) by metal affinity chromatography and gel filtration
recombinant protein, purified to homogeneity from the soluble fraction by HiTrap chelating chromatography
SP-Toyopearl column chromatography, Q-Sepharose column chromatography, PPG-Toyopearl column chromatography, and SuperSW3000 gel filtration
TSK gel filtration
-