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1.4.1.4: glutamate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about glutamate dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.4.1.4

Reaction

L-glutamate
+
H2O
 +
NADP+
=
2-oxoglutarate
 +
NH3
 +
NADPH
 +
H+

Synonyms

APE1386, BpNADPGDH I, BpNADPGDH II, dehydrogenase, glutamate (nicotinamide adenine dinucleotide phosphate), EcGDH, GDH, GDH1, Gdh1p, GDH2, GDH3, Gdh3p, GDH4, GdhA, GDHB, GDHC, GDHI, GDHI', GDHII, GDHP, GluDH, glutamate dehydrogenase, glutamate dehydrogenase 1, glutamate dehydrogenase 2, glutamate dehydrogenase 3, glutamic acid dehydrogenase, glutamic dehydrogenase, GW612_14215, H-form specific BpNADPGDH II, hGDH, hyphal-form specific BpNADPGDH II, L-glutamate dehydrogenase, NAD(P)H-dependent glutamate dehydrogenase, NADP(+)-dependent glutamate dehydrogenase, NADP(H)-dependent glutamate dehydrogenase, NADP(H)-GDH, NADP(H)-specific glutamate dehydrogenase, NADP+ - dependant-glutamate dehydrogenase, NADP+-dependent GDH, NADP+-dependent glutamate dehydrogenase, NADP+-Gdh, NADP-dependent GDH, NADP-dependent GluDH, NADP-dependent glutamate dehydrogenase, NADP-dependent glutamate dehydrogenase 1, NADP-dependent glutamate dehydrogenase 2, NADP-GDH, NADP-GDH 1, NADP-GDH 2, NADP-glutamate dehydrogenase, NADP-linked glutamate dehydrogenase, NADP-specific glutamate dehydrogenase, NADP-specific L-glutamate dehydrogenase, NADP-ylGdh1p, NADPH-dependent GDH, NADPH-dependent glutamate dehydrogenase, OsGDH4, Pcal_1606, RocG, TrGDH, Y-form specific BpNADPGDH I, YALI0F17820g, yeast-form specific BpNADPGDH I, ylGDH1

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.4 glutamate dehydrogenase (NADP+)

Storage Stability

Storage Stability on EC 1.4.1.4 - glutamate dehydrogenase (NADP+)

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 40% v/v glycerol, no activity loss
-
-20°C, phosphate buffer at pH 7.0, 0.2 mM 2-mercaptoethanol, 20 h, complete activity loss
-
-20°C, potassium phosphate buffer, pH 7.4, containing 0.01% 2-mercaptoethanol and 10% glycerol, 100% activity
-
0-4°C, after 15 or 30 days, 18% or 2% activity
-
0-4°C, Tris-HCl buffer, pH 9.0, 2 months, 100% activity
-
0°C, at the low stock enzyme dilutions necessary for kinetic assays GDH loses activity over the duration of an experiment, at 0.01 and 0.02 mg/ml GDH retains 90-95% activity over 24 h in glass tubes, whereas this declines with decreasing storage concentration (to 87%, 71%, and 47% at concentrations of GDH of 5, 2 and 0.5 mg/ml, respectively)
-
25°C, 20 mM potassium phosphate, pH 6.5, containing 7 mM 2-mercaptoethanol and 10% glycerol, stable for several weeks
-
25°C, 20% v/v glycerol, 476 h, 50% activity
-
4°C after 7 days, 55% activity
-
4°C and -25°C, 0.2 M Tris/HCl buffer, pH 7.4, 24 h or 2 months respectively, 100% activity
-
4°C, 0.1 M phosphate buffer, several days, no loss of activity
-
4°C, 1 week
-
4°C, 10 mM potassium phosphate buffer, pH 7.2, 0.1 mM EDTA, 0.1 mM DTT, 5 months, no activity loss
4°C, 2 months, no activity loss
-
4°C, 2 months, no loss of activity
4°C, 20 mM potassium phosphate, pH 6.5, containing 7 mM 2-mercaptoethanol and 10% glycerol, stable for several weeks
-
4°C, purified enzyme, stable for about 5 to 6 months without any significant loss of activity
-
4°C, purified enzymes, stable for about 5 to 6 months without any significant loss of activity
-
4°C, stable for at least 2 months
4°C, ten days and 25°C, 15 h, both 100% activity
-
4°C, water, 20 mM potassium phosphate or sodium phosphate, pH 7.2, at least 3 months
-
5°C, phosphate buffer at pH 7.0, 2 mM 2-mercaptoethanol, 6 months, no activity loss
-
fully active at 4 M concentration NaCl at room temperature for months
-
room temperature, several months stable
-