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1.4.1.4: glutamate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about glutamate dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.4.1.4

Reaction

L-glutamate
+
H2O
 +
NADP+
=
2-oxoglutarate
 +
NH3
 +
NADPH
 +
H+

Synonyms

APE1386, BpNADPGDH I, BpNADPGDH II, dehydrogenase, glutamate (nicotinamide adenine dinucleotide phosphate), EcGDH, GDH, GDH1, Gdh1p, GDH2, GDH3, Gdh3p, GDH4, GdhA, GDHB, GDHC, GDHI, GDHI', GDHII, GDHP, GluDH, glutamate dehydrogenase, glutamate dehydrogenase 1, glutamate dehydrogenase 2, glutamate dehydrogenase 3, glutamic acid dehydrogenase, glutamic dehydrogenase, GW612_14215, H-form specific BpNADPGDH II, hGDH, hyphal-form specific BpNADPGDH II, L-glutamate dehydrogenase, NAD(P)H-dependent glutamate dehydrogenase, NADP(+)-dependent glutamate dehydrogenase, NADP(H)-dependent glutamate dehydrogenase, NADP(H)-GDH, NADP(H)-specific glutamate dehydrogenase, NADP+ - dependant-glutamate dehydrogenase, NADP+-dependent GDH, NADP+-dependent glutamate dehydrogenase, NADP+-Gdh, NADP-dependent GDH, NADP-dependent GluDH, NADP-dependent glutamate dehydrogenase, NADP-dependent glutamate dehydrogenase 1, NADP-dependent glutamate dehydrogenase 2, NADP-GDH, NADP-GDH 1, NADP-GDH 2, NADP-glutamate dehydrogenase, NADP-linked glutamate dehydrogenase, NADP-specific glutamate dehydrogenase, NADP-specific L-glutamate dehydrogenase, NADP-ylGdh1p, NADPH-dependent GDH, NADPH-dependent glutamate dehydrogenase, OsGDH4, Pcal_1606, RocG, TrGDH, Y-form specific BpNADPGDH I, YALI0F17820g, yeast-form specific BpNADPGDH I, ylGDH1

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.4 glutamate dehydrogenase (NADP+)

Purification

Purification on EC 1.4.1.4 - glutamate dehydrogenase (NADP+)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation, Procion Red HE-3B Sepharose CL-6B column chromatography and Q-Sepharose column chromatography
-
by Cibacron red LS-B dye-affinity purification with a step KCl gradient elution, 17fold purified
-
by Cibacron red LS-B dye-affinity purification with a step KCl gradient elution, more than 7fold purified to homogeneity
-
by gel filtration
-
co-purification with glutathione disulfide reductase
-
native GDHI' 142fold from cells grown on isophthalate and 510fold from cells grown on 2YT
-
native isozymes GDHI and GDHII from strain PP4 grown on isophthalate and glucose, respectively. Enzyme yields are better in the presence of 1 mM 2-oxoglutarate, 1 mM EDTA, and 4.35% glycerol. GDHI and GDHII are purified 228fold and 428fold, respectively
-
of the alpha-subunit, beta-subunit and a truncated subunit missing 40 amino acids at their N-terminus expressed in Escherichia coli
-
purified to homogeneity using novel reactive dye-affinity resins
recombinant enzyme
recombinant His-tagged isozyme BpNADPGDH I 36fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged isozyme BpNADPGDH II 41fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged GDH2 from Escherichia coli strain KRX by nickel affinity chromatography and gel filtration
recombinant His6-tagged GdhA from Escherichia coli by nickel affinity chromatography
-
recombinant N-terminally Met-Ala-Ser-tagged and C-terminally His6-tagged enzyme from Eschericia coli strain Rosetta(DE3) by affinity chromatography and gel filtration
recombinant wild-type and mutant enzymes
under semianoxically conditions
-
using dye-affintiy chromatography and anion-exchange chromatography
-