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0.004 - 606
2-oxoglutarate
0.0417 - 1349
L-glutamate
3.629
NAD+
-
pH 9.0, 50°C
additional information
additional information
-
0.004
2-oxoglutarate
-
pH not specified in the publication, 30°C
0.018
2-oxoglutarate
-
pH 8.5, 40°C
0.16
2-oxoglutarate
-
reductive amination at 80°C
0.2
2-oxoglutarate
-
reductive amination
0.2
2-oxoglutarate
pH 7.0, 25°C, recombinant enzyme
0.27
2-oxoglutarate
pH 8.0, 80°C
0.28
2-oxoglutarate
-
reductive amination
0.31
2-oxoglutarate
-
isoform Gdh1, pH 5.8, 30°C
0.32
2-oxoglutarate
-
reductive amination
0.34
2-oxoglutarate
-
reductive amination at 25°C
0.34
2-oxoglutarate
-
pH 5.8, 30°C
0.36
2-oxoglutarate
-
reductive amination
0.365
2-oxoglutarate
pH 7.0, 25°C, recombinant enzyme
0.37
2-oxoglutarate
-
isoform Gdh1, pH 7.5, 30°C
0.4
2-oxoglutarate
-
reductive amination
0.4
2-oxoglutarate
-
isoform Gdh3, pH 7.5, 30°C
0.46
2-oxoglutarate
-
pH 7.5, 30°C
0.5
2-oxoglutarate
-
reductive amination
0.5
2-oxoglutarate
-
reductive amination at 60°C
0.64
2-oxoglutarate
-
reductive amination
0.64
2-oxoglutarate
-
reductive amination
0.68
2-oxoglutarate
-
in the presence of 100 mM NH4Cl, at 25°C and pH 8
0.68
2-oxoglutarate
-
wild-type EcGDH, Vmax: 464 micromol/min/mg, pH 8.0, 25°C
0.77
2-oxoglutarate
-
reductive amination
0.89
2-oxoglutarate
wild-type, pH 7.0,30°C
0.93
2-oxoglutarate
-
reductive amination
1
2-oxoglutarate
-
reductive amination
1.1
2-oxoglutarate
-
in the presence of 200 mM NH4Cl, at 25°C and pH 8
1.2
2-oxoglutarate
-
reductive amination
1.25
2-oxoglutarate
-
reductive amination
1.3
2-oxoglutarate
Thermophilic bacillus
-
reductive amination
1.48
2-oxoglutarate
-
isoform Gdh3, S0.5 value, Hill coefficient 2.4, pH 5.8, 30°C
1.5
2-oxoglutarate
-
reductive amiantion
1.54
2-oxoglutarate
-
reductive amination
1.7
2-oxoglutarate
-
reductive amination at 50°C
1.7
2-oxoglutarate
-
reductive amination at 80°C
1.7
2-oxoglutarate
pH 9.0, 50°C
1.7
2-oxoglutarate
pH 8.3, 50°C
1.7
2-oxoglutarate
pH 9.5, 50°C, recombinant enzyme
1.95
2-oxoglutarate
-
S0.5 value, Hill coefficient 4, pH 5.8, 30°C
2
2-oxoglutarate
-
reductive amination at 0.6 M NH4Cl
2
2-oxoglutarate
-
reductive amination at 56°C
2.2
2-oxoglutarate
-
pH 8.0, 25°C
2.41
2-oxoglutarate
-
reductive amination
2.5 - 3
2-oxoglutarate
-
in the presence of 400 mM NH4Cl, at 25°C and pH 8
3
2-oxoglutarate
-
reductive amination at 25°C
3
2-oxoglutarate
-
reductive amination at 25°C
3.13
2-oxoglutarate
-
reductive amination at 30°C
3.2
2-oxoglutarate
-
reductive amination at 33°C
3.2
2-oxoglutarate
-
pH 7.0, 38.5°C, DELTA40N-homohexamer
3.25
2-oxoglutarate
-
reductive amination
3.61
2-oxoglutarate
-
S0.5 value, Hill coefficient 4.4, pH 7.5, 30°C
3.97
2-oxoglutarate
-
pH 8.0, 30°C, recombinant enzyme
4
2-oxoglutarate
-
reductive amination
4
2-oxoglutarate
mutant C141S, S0.5 value, Hill coefficient 2.3, pH 8.0, temperature not specified in the publication
4.73
2-oxoglutarate
AKQ74236
25°C, pH 7.5
4.78
2-oxoglutarate
-
28°C
4.78
2-oxoglutarate
wild-type, S0.5 value, Hill coefficient 3.2, pH 8.0, temperature not specified in the publication
5
2-oxoglutarate
-
reductive amination
5
2-oxoglutarate
-
pH 7.0, 38.5°C, alpha-homohexamer
5.6
2-oxoglutarate
-
reductive amination
5.7
2-oxoglutarate
recombinant wild-type, S0.5 value, Hill coefficient 3.5, pH 8.0, temperature not specified in the publication
6
2-oxoglutarate
-
pH 7.6, 60°C
6.8
2-oxoglutarate
-
pH 7.0, 38.5°C, beta-homohexamer
9
2-oxoglutarate
-
reductive amination at 1 M NH4Cl
15.5
2-oxoglutarate
mutant C415S, S0.5 value, Hill coefficient 4.9, pH 8.0, temperature not specified in the publication
25.1
2-oxoglutarate
wild-type inactivated by 2-hydroxyethyl disulfide, S0.5 value, Hill coefficient 2.4, pH 8.0, temperature not specified in the publication
285
2-oxoglutarate
-
chimeric protein CEC consisting of the substrate-binding domain of CsGDH and the coenzyme-binding domain of Escherichia coli GDH, 781 mM NH4Cl, Vmax: 2260 micromol/min/mg, pH 8.0, 25°C
606
2-oxoglutarate
-
chimeric protein CEC consisting of the substrate-binding domain of CsGDH and the coenzyme-binding domain of Escherichia coli GDH, 1800 mM NH4Cl, Vmax: 200 micromol/min/mg, pH 8.0, 25°C
0.0417
L-glutamate
-
pH not specified in the publication, 30°C
0.22
L-glutamate
-
oxidative deamination at 80°C
0.24
L-glutamate
pH 8.0, 22°C, recombinant mutant K341L
0.45
L-glutamate
pH 8.0, 22°C, recombinant wild-type enzyme
0.6
L-glutamate
-
pH 7.6, 60°C
0.6
L-glutamate
pH 8.0, 22°C, recombinant mutant C321A
0.61
L-glutamate
pH 8.0, 25°C, recombinant enzyme
0.9
L-glutamate
pH 8.0, 22°C, recombinant mutant P320A
1.05
L-glutamate
pH 8.0, 25°C, recombinant enzyme
1.1
L-glutamate
Thermophilic bacillus
-
oxidative deamination
1.3
L-glutamate
-
oxidative deamination
1.3
L-glutamate
pH 8.0, 80°C
2
L-glutamate
-
reductive amination
2.2
L-glutamate
-
oxidative deamination
2.3
L-glutamate
-
oxidative deamination
2.3
L-glutamate
-
oxidative deamination
2.3
L-glutamate
-
at 25°C and pH 8
2.3
L-glutamate
-
wild-type EcGDH, Vmax: 37.9 micromol/min/mg, pH 8.0, 25°C
3.2
L-glutamate
-
oxidative deamination at 25°C
3.3
L-glutamate
-
oxidative deamination at 50°C
3.3
L-glutamate
pH 8.3, 50°C
3.4
L-glutamate
pH 10.5, 50°C, recombinant enzyme
3.4
L-glutamate
pH 9.5, 50°C
3.7
L-glutamate
-
reductive amination
3.9
L-glutamate
-
oxidative deamination at 60°C
5.18
L-glutamate
-
oxidative deamination
5.5
L-glutamate
-
oxidative deamination
6.06
L-glutamate
-
oxidative deamination at 30°C
6.36
L-glutamate
-
oxidative deamination, Gdh3p gene
9.12
L-glutamate
-
oxidative deamination at 80°C
9.79
L-glutamate
-
oxidative deamination, Gdh1p gene
10
L-glutamate
-
oxidative deamination
14.2
L-glutamate
-
oxidative deamination
15.15
L-glutamate
-
pH 8.0, 30°C, recombinant enzyme
18
L-glutamate
-
oxidative deamination, biphasic kinetics
21
L-glutamate
-
pH 7.0, 38.5°C, beta-homohexamer
23.29
L-glutamate
AKQ74236
25°C, pH 7.5
25
L-glutamate
-
pH 7.0, 38.5°C, DELTA40N-homohexamer
27
L-glutamate
-
oxidative deamination at 33°C
28.6
L-glutamate
-
oxidative deamination
28.6
L-glutamate
mutant C141S, pH 9.3, temperature not specified in the publication
29
L-glutamate
-
oxidative deamination
30.9
L-glutamate
recombinant wild-type, pH 9.3, temperature not specified in the publication
32.3
L-glutamate
-
oxidative deamination
34
L-glutamate
-
pH 7.0, 38.5°C, alpha-homohexamer
34.6
L-glutamate
wild-type, pH 9.3, temperature not specified in the publication
36.8
L-glutamate
mutant C415S, pH 9.3, temperature not specified in the publication
38.2
L-glutamate
-
oxidative deamination
44
L-glutamate
-
oxidative deamination
49.8
L-glutamate
wild-type inactivated by 2-hydroxyethyl disulfide, S0.5 value, Hill coefficient 1.9, pH 9.3, temperature not specified in the publication
50
L-glutamate
wild-type, pH 7.0,30°C
67.4
L-glutamate
-
oxidative deamination
79
L-glutamate
-
oxidative deamination
81
L-glutamate
-
oxidative deamination, biphasic kinetics
225
L-glutamate
-
oxidative deamination
1349
L-glutamate
-
chimeric protein CEC consisting of the substrate-binding domain of CsGDH and the coenzyme-binding domain of Escherichia coli GDH, Vmax: 121.9 micromol/min/mg, pH 8.0, 25°C
0.16
NADH
wild-type, pH 7.0,30°C
0.368
NADH
-
pH 9.0, 50°C
0.69
NADH
mutant K136A, pH 7.0,30°C
0.00007
NADP+
-
pH not specified in the publication, 30°C
0.0064
NADP+
-
oxidative deamination
0.0098
NADP+
-
recombinant enzyme, oxidative deamination
0.01
NADP+
-
oxidative deamination at 25°C
0.0102
NADP+
-
wild-type enzyme, oxidative deamination
0.0105
NADP+
-
oxidative deamination, Gdh3p gene
0.013
NADP+
-
oxidative deamination
0.013
NADP+
-
oxidative deamination
0.0141
NADP+
-
oxidative deamination, Gdh1p gene
0.015
NADP+
-
pH 7.0, 38.5°C, DELTA40N-homohexamer
0.017
NADP+
wild-type, pH 9.3, temperature not specified in the publication
0.018
NADP+
-
wild-type EcGDH, Vmax: 45.6 micromol/min/mg, pH 8.0, 25°C
0.0184
NADP+
wild-type enzyme, pH 8.0, temperature not specified in the publication
0.019
NADP+
pH 8.0, 25°C, recombinant enzyme
0.02
NADP+
-
pH 7.6, 60°C
0.02
NADP+
pH 8.0, 25°C, recombinant enzyme
0.022
NADP+
wild-type inactivated by 2-hydroxyethyl disulfide, pH 9.3, temperature not specified in the publication
0.023
NADP+
-
oxidative deamination
0.027
NADP+
-
pH 7.0, 38.5°C, alpha-homohexamer
0.028
NADP+
-
pH 7.0, 38.5°C, beta-homohexamer
0.028
NADP+
recombinant wild-type, pH 9.3, temperature not specified in the publication
0.029
NADP+
-
oxidative deamination at 80°C
0.029
NADP+
-
oxidative deamination at 30°C
0.03
NADP+
-
oxidative deamination
0.031
NADP+
-
oxidative deamination
0.0328
NADP+
mutant K289Q, pH 8.0, temperature not specified in the publication
0.035
NADP+
pH 10.5, 50°C, recombinant enzyme
0.038
NADP+
-
oxidative deamination at 80°C
0.039
NADP+
-
oxidative deamination
0.039
NADP+
-
oxidative deamination at 50°C
0.04
NADP+
-
oxidative deamination
0.04
NADP+
-
oxidative deamination
0.041
NADP+
-
pH 8.0, 30°C, recombinant enzyme
0.042
NADP+
-
oxidative deamination
0.043
NADP+
-
oxidative deamination
0.043
NADP+
-
oxidative deamination
0.044
NADP+
-
oxidative deamination
0.045
NADP+
-
oxidative deamination
0.05
NADP+
-
oxidative deamination
0.053
NADP+
-
oxidative deamination
0.06
NADP+
-
oxidative deamination at 60°C
0.061
NADP+
-
oxidative deamination
0.07
NADP+
pH 8.0, 22°C, recombinant wild-type enzyme
0.0819
NADP+
mutant K292Q, pH 8.0, temperature not specified in the publication
0.088
NADP+
-
pH 9.0, 50°C
0.093
NADP+
wild-type, pH 7.0,30°C
0.1 - 1
NADP+
pH 8.0, 80°C
0.11
NADP+
-
oxidative deamination
0.117
NADP+
-
oxidative deamination at 33°C
0.12
NADP+
-
oxidative deamination
0.12
NADP+
-
oxidative deamination
0.129
NADP+
mutant K286Q, pH 8.0, temperature not specified in the publication
0.13
NADP+
pH 8.0, 22°C, recombinant mutant K341L
0.15
NADP+
pH 8.0, 22°C, recombinant mutant C321A
0.163
NADP+
-
chimeric protein CEC consisting of the substrate-binding domain of CsGDH and the coenzyme-binding domain of Escherichia coli GDH, Vmax: 80.8 micromol/min/mg, pH 8.0, 25°C
0.3
NADP+
Thermophilic bacillus
-
oxidative deamination
0.3
NADP+
pH 8.0, 22°C, recombinant mutant P320A
0.929
NADP+
mutant K286Q/R289Q/R292Q , pH 8.0, temperature not specified in the publication
18.3
NADP+
mutant K286Q/R289Q/R292Q/S264L/S240A, pH 8.0, temperature not specified in the publication
18.4
NADP+
-
at 25°C and pH 8
19.06
NADP+
mutant K286Q/R289Q/R292Q/S264L, pH 8.0, temperature not specified in the publication
0.00013
NADPH
-
pH not specified in the publication, 30°C
0.003
NADPH
-
reductive amination
0.004
NADPH
-
reductive amination
0.007
NADPH
pH 7.0, 25°C, recombinant enzyme
0.0087
NADPH
-
reductive amination
0.0097
NADPH
-
reductive amination
0.01
NADPH
-
reductive amination
0.011
NADPH
wild-type, pH 8.0, temperature not specified in the publication
0.0113
NADPH
-
reductive amination, Gdh1p gene
0.012
NADPH
-
pH 7.6, 60°C
0.012
NADPH
pH 7.0, 25°C, recombinant enzyme
0.016
NADPH
wild-type, pH 7.0,30°C
0.017
NADPH
-
reductive amination
0.017
NADPH
pH 9.5, 50°C, recombinant enzyme
0.018
NADPH
-
reductive amination at 25°C
0.019
NADPH
-
reductive amination
0.02
NADPH
-
reductive amination
0.02
NADPH
-
reductive amination at 60°C
0.021
NADPH
-
pH 8.0, 25°C
0.022
NADPH
-
reductive amination at 50°C
0.023
NADPH
recombinant wild-type, pH 8.0, temperature not specified in the publication
0.024
NADPH
-
reductive amination
0.027
NADPH
-
reductive amination
0.027
NADPH
-
reductive amination
0.028
NADPH
-
reductive amination
0.03
NADPH
-
reductive amination at 25°C
0.0331
NADPH
-
reductive amination, Gdh3p gene
0.035
NADPH
-
reductive amination
0.039
NADPH
-
S0.5 value, Hill coefficient 1.8, pH 7.5, 30°C
0.04
NADPH
-
reductive amination
0.042
NADPH
-
isoform Gdh3, pH 7.5, 30°C
0.044
NADPH
-
reductive amination at 30°C
0.044
NADPH
wild-type inactivated by 2-hydroxyethyl disulfide, pH 8.0, temperature not specified in the publication
0.045
NADPH
-
isoform Gdh1, pH 7.5, 30°C
0.046
NADPH
-
pH 7.5, 30°C
0.049
NADPH
-
reductive amination
0.049
NADPH
-
reductive amination
0.053
NADPH
Thermophilic bacillus
-
reductive amination
0.058
NADPH
mutant K1376A, pH 7.0,30°C
0.06
NADPH
-
wild-type EcGDH, Vmax: 503 micromol/min/mg, pH 8.0, 25°C
0.064
NADPH
-
reductive amination
0.066
NADPH
-
reductive amination at 80°C
0.07
NADPH
-
reductive amination
0.073
NADPH
mutant R290A, pH 7.0,30°C
0.074
NADPH
-
reductive amination at 33°C
0.075
NADPH
-
reductive amination at 70°C
0.078
NADPH
-
reductive amination
0.083
NADPH
-
reductive amination
0.095
NADPH
-
reductive amination
0.11
NADPH
-
reductive amination
0.111
NADPH
AKQ74236
25°C, pH 7.5
0.125
NADPH
-
pH 7.0, 38.5°C, DELTA40N-homohexamer
0.14
NADPH
-
reductive amination at 80°C
0.14
NADPH
-
pH 7.0, 38.5°C, alpha-homohexamer
0.16
NADPH
mutant S265A, pH 7.0,30°C
0.26
NADPH
-
pH 7.0, 38.5°C, beta-homohexamer
0.28
NADPH
-
pH 9.0, 50°C
0.34
NADPH
-
pH 8.0, 30°C, recombinant enzyme
0.51
NADPH
-
chimeric protein CEC consisting of the substrate-binding domain of CsGDH and the coenzyme-binding domain of Escherichia coli GDH, Vmax: 2180 micromol/min/mg, pH 8.0, 25°C
59.7
NADPH
-
at 25°C and pH 8
0.00056
NH3
-
recombinant enzyme, reductive amination
0.00065
NH3
-
wild-type enzyme, reductive amination
1.05
NH3
wild-type, pH 8.0, temperature not specified in the publication
1.1
NH3
recombinant wild-type, pH 8.0, temperature not specified in the publication
1.16
NH3
-
pH 8.0, 30°C, recombinant enzyme
1.2
NH3
mutant C141S, pH 8.0, temperature not specified in the publication
1.48
NH3
-
pH 8.0, 25°C, recombinant enzyme
1.89
NH3
-
in the presence of 20 mM 2-oxoglutarate, at 25°C and pH 8
2.2
NH3
pH 9.5, 50°C, recombinant enzyme
2.47
NH3
-
in the presence of 5 mM 2-oxoglutarate, at 25°C and pH 8
2.5
NH3
-
reductive amination
2.5 - 3
NH3
-
in the presence of 2.5 mM 2-oxoglutarate, at 25°C and pH 8
2.6
NH3
wild-type inactivated by 2-hydroxyethyl disulfide, pH 8.0, temperature not specified in the publication
4
NH3
-
reductive amination
4
NH3
-
reductive amination at 60°C
4.56
NH3
-
reductive amination
5
NH3
-
reductive amination, Gdh3p gene
5.96
NH3
-
reductive amination, Gdh1p gene
5.96
NH3
-
reductive amination at 30°C
6.9
NH3
-
isoform Gdh3, pH 7.5, 30°C
8.6
NH3
-
isoform Gdh1, pH 7.5, 30°C
21.4
NH3
-
S0.5 value, Hill coefficient 2.7, pH 7.5, 30°C
33
NH3
wild-type, pH 7.0,30°C
119
NH3
-
reductive amination at 70°C
0.29
NH4+
-
reductive amination
0.37
NH4+
-
reductive amination
0.63
NH4+
-
reductive amination at 80°C
1.1
NH4+
-
reductive amination
1.7
NH4+
-
reductive amination
2
NH4+
-
reductive amination
2.1
NH4+
-
reductive amination at 33°C
2.2
NH4+
-
reductive amination
2.2
NH4+
-
reductive amination at 25°C
2.5 - 3
NH4+
-
wild-type EcGDH, Vmax: 298 micromol/min/mg, pH 8.0, 25°C
2.6
NH4+
-
reductive amination, biphasic kinetics
3.3
NH4+
-
reductive amination alpha-isoenzyme, depending on NADPH-concentration
3.7
NH4+
-
reductive amination
4.2
NH4+
-
reductive amination at 25°C
6.5
NH4+
-
reductive amination
6.6
NH4+
-
pH 7.0, 38.5°C, alpha-homohexamer
6.76
NH4+
-
reductive amination
7.5
NH4+
-
reductive amination at 56°C
7.7
NH4+
-
reductive amination
9
NH4+
-
reductive amination
9.2
NH4+
-
reductive amination
10
NH4+
-
reductive amination
10.8
NH4+
-
pH 7.0, 38.5°C, DELTA40N-homohexamer
15.5
NH4+
-
reductive amination at 80°C
16.6
NH4+
-
reductive amination
20
NH4+
-
reductive amination
21
NH4+
Thermophilic bacillus
-
reductive amination
21.2
NH4+
-
reductive amination
21.2
NH4+
-
biphasic kinetics
22
NH4+
-
reductive amination
30.8
NH4+
-
reductive amination
36
NH4+
-
reductive amination
66
NH4+
-
reductive amination
75
NH4+
-
reductive amination beta-isoenzyme
80
NH4+
-
pH 7.0, 38.5°C, beta-homohexamer
83
NH4+
-
reductive amination at 50°C
304
NH4+
-
chimeric protein CEC consisting of the substrate-binding domain of CsGDH and the coenzyme-binding domain of Escherichia coli GDH, Vmax: 1960 micromol/min/mg, pH 8.0, 25°C
416
NH4+
-
reductive amination
additional information
additional information
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kinetic analysis, overview, the isozyme GDHI' shows negative cooperativity with NH4+ substrate, with a decreased affinity for NH4Cl
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additional information
additional information
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kinetic analysis, overview, the isozymes show negative cooperativity with NH4+ substrate, with a decreased affinity for NH4Cl
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additional information
additional information
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Lineweaver-Burk kinetics
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