1.4.1.4: glutamate dehydrogenase (NADP+) This is an abbreviated version! For detailed information about glutamate dehydrogenase (NADP+), go to the full flat file .
Reaction
L-glutamate +
H2O +
NADP+ =
2-oxoglutarate +
NH3 +
NADPH +
H+
Synonyms APE1386, BpNADPGDH I, BpNADPGDH II, dehydrogenase, glutamate (nicotinamide adenine dinucleotide phosphate), EcGDH, GDH, GDH1, Gdh1p, GDH2, GDH3, Gdh3p, GDH4, GdhA, GDHB, GDHC, GDHI, GDHI', GDHII, GDHP, GluDH, glutamate dehydrogenase, glutamate dehydrogenase 1, glutamate dehydrogenase 2, glutamate dehydrogenase 3, glutamic acid dehydrogenase, glutamic dehydrogenase, GW612_14215, H-form specific BpNADPGDH II, hGDH, hyphal-form specific BpNADPGDH II, L-glutamate dehydrogenase, NAD(P)H-dependent glutamate dehydrogenase, NADP(+)-dependent glutamate dehydrogenase, NADP(H)-dependent glutamate dehydrogenase, NADP(H)-GDH, NADP(H)-specific glutamate dehydrogenase, NADP+ - dependant-glutamate dehydrogenase, NADP+-dependent GDH, NADP+-dependent glutamate dehydrogenase, NADP+-Gdh, NADP-dependent GDH, NADP-dependent GluDH, NADP-dependent glutamate dehydrogenase, NADP-dependent glutamate dehydrogenase 1, NADP-dependent glutamate dehydrogenase 2, NADP-GDH, NADP-GDH 1, NADP-GDH 2, NADP-glutamate dehydrogenase, NADP-linked glutamate dehydrogenase, NADP-specific glutamate dehydrogenase, NADP-specific L-glutamate dehydrogenase, NADP-ylGdh1p, NADPH-dependent GDH, NADPH-dependent glutamate dehydrogenase, OsGDH4, Pcal_1606, RocG, TrGDH, Y-form specific BpNADPGDH I, YALI0F17820g, yeast-form specific BpNADPGDH I, ylGDH1
ECTree
Activating Compound
Activating Compound on EC 1.4.1.4 - glutamate dehydrogenase (NADP+)
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3,5-pyrazoledicarboxylate
-
activates at lower 2-oxoglutarate concentration
3,5-pyridinedicarboxylate
-
activates at lower 2-oxoglutarate concentration
DL-2-hydroxyglutarate
-
activates at lower 2-oxoglutarate concentration
ethanol
-
activates up to 40% v/v
Glutarate
-
activates at lower 2-oxoglutarate concentration
IDP
-
at 1 mM five-fold Michaelis Menten constant of 2-oxoglutarate
Itaconate
-
activates even in the presence of 10 mM 2-oxoglutarate
K2HPO4
-
at 0.15 M three to fourfold stimulation of activity
K2SO4
-
280-300% activity at 150-200 mM
K3PO4
-
less effective than K2SO4 and Na3PO4
MgCl2
-
at 5 mM, 104% activity, oxidative deamination
MnCl2
-
at 5 mM, 250% activity, oxidative deamination
Na2SO4
-
less effective than K2SO4 and Na3PO4
Na3PO4
-
280-300% activity at 150-200 mM
oxalylglycine
-
activates at lower 2-oxoglutarate concentration
potassium phosphate
-
up to 0.1 M oxidative deamination
UTP
-
activates up to 70%
Zn2+
-
activates up to 0.1 mM
additional information
-
exhibits 2.5-fold-higher GDH activity than cells are grown in milk, instead of glucose
-
ADP
-
at 1 mM, 11% activation
ADP
-
at 0.3 mM 40% activation
AMP
-
slight activation up to 1 mM
AMP
-
slight activation up to 1 mM
ATP
-
30% stimulation in the presence of 2 mM Mg2+
ATP
-
at 2 mM, 50% activation
ATP
-
at 0.3 mM, 60% activation of reductive amination
ATP
-
at 0.8 M, 68% activation
CaCl2
-
at 20 mM, 25% and 108% activation of reductive amination and oxidative deamination respectively
CaCl2
-
at 5 mM, 135% activation, oxidative deamination
GDP
-
30% stimulation in the presence of 2 mM Mg2+
GDP
-
at 1 mM five-fold Michaelis Menten constant of 2-oxoglutarate
GTP
-
30% stimulation in the presence of 2 mM Mg2+
GTP
-
at 0.083 mM 22% activation
KCl
-
170-200% activity at 50-100 mM
KCl
-
at 0.2 M three to fourfold stimulation of activity
KCl
-
optimal activity at 1-1.5 M
KCl
-
at concentrations below 2 mM
KCl
-
at concentrations below 1 mM
NaCl
-
170-200% activity at 50-100 mM
NaCl
-
at 0.2 M three to fourfold stimulation of activity
NaCl
-
optimal activity at 1-1.5 M
NH4+
-
the activity of glutamate dehydrogenase (GDH) in the ammonium-tolerant species Myriophyllum spicatum leaves performs a dose-response curve (increase of 103% for NADPH-dependent GDH) with the [NH4+-N] increasing from 0 to 100 mg/l while glutamine synthetase (GS) activity slightly changes. Compared to that, for the ammonium-sensitive species, Potamogeton lucens, the activity of GDH records no major changes, while the GS increases slightly (17%)
NH4+
-
the activity of glutamate dehydrogenase (GDH) in the ammonium-tolerant species Myriophyllum spicatum leaves performs a dose-response curve (increase of 103% for NADPH-dependent GDH) with the [NH4+-N] increasing from 0 to 100 mg/l while glutamine synthetase (GS) activity slightly changes. Compared to that, for the ammonium-sensitive species, Potamogeton lucens, the activity of GDH records no major changes, while the GS increases slightly (17%)