1.4.1.3: glutamate dehydrogenase [NAD(P)+]
This is an abbreviated version!
For detailed information about glutamate dehydrogenase [NAD(P)+], go to the full flat file.
Reaction
Synonyms
At5g07440, At5g18170, dehydrogenase, glutamate (nicotinamide adenine dinucleotide (phosphate)), dual-coenzyme specific glutamate dehydrogenase, GDH, gdh-1, GDH1, GDH2, GDH3, GdhA, gdhA_1, GDHB, GDHII, GLDH, GLUD1, GLUD2, GluDH, glutamate dehydrogenase, glutamate dehydrogenase 1, glutamate dehydrogenase 2, glutamic acid dehydrogenase, glutamic dehydrogenase, hGDH1, hGDH2, hGLUD1, hGLUD2, housekeeping glutamate dehydrogenase, L-glutamate dehydrogenase, L-glutamic acid dehydrogenase, Legdh1, Membrane protein 50, MP50, NAD(P)+-dependent glutamate dehydrogenase, NAD(P)-dependent GDH, NAD(P)-dependent glutamate dehydrogenase, NAD(P)-glutamate dehydrogenase, NAD(P)H-dependent glutamate dehydrogenase, NAD(P)H-utilizing glutamate dehydrogenase, NADH-GDH, NADH-glutamate dehydrogenase, TTC1211, TTC1212, TtGDH
ECTree
1.4.1.3 glutamate dehydrogenase [NAD(P)+]Advanced search results
results (
results (Substrates Products
Substrates Products on EC 1.4.1.3 - glutamate dehydrogenase [NAD(P)+]
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-aminobutyrate + H2O + NADP+
2-oxobutyrate + NH3 + NADPH
-
3% of activity with L-glutamate
-
?
2-oxoglutarate + NH3 + NAD(P)H + H+
L-glutamate + H2O + NAD(P)+
-
-
-
r
L-glutamate + H2O + 2-azido-NAD+
2-oxoglutarate + NH3 + 2-azido-NADH
-
-
-
?
L-glutamate + H2O + N6-(2-aminoethyl)-NAD(P)+
2-oxoglutarate + NH3 + N6-(2-aminoethyl)-NAD(P)H
-
-
-
?
L-glutamate + H2O + N6-(2-aminoethyl)-NAD+
2-oxoglutarate + NH3 + N6-(2-aminoethyl)-NADH + H+
-
-
-
-
r
L-glutamate + H2O + N6-(2-hydroxy-3-trimethylammoniumpropyl)-NAD+
2-oxoglutarate + NH3 + N6-(2-hydroxy-3-trimethylammoniumpropyl)-NADH
-
-
-
?
L-glutamate + H2O + N6-(2-hydroxy-3-trimethylammoniumpropyl)-NAD+
2-oxoglutarate + NH3 + N6-(2-hydroxy-3-trimethylammoniumpropyl)-NADH + H+
-
-
-
-
r
L-glutamate + H2O + N6-(3-sulfonatopropyl)-NAD+
2-oxoglutarate + NH3 + N6-(2-sulfonatopropyl)-NADH
-
-
-
?
L-glutamate + H2O + N6-poly(ethyleneglycol)-N6-(2-aminoethyl)-NAD+
2-oxoglutarate + NH3 + )poly(ethyleneglycol)-N6-(2-aminoethyl)-NADH + H+
-
-
-
-
r
L-glutamate + H2O + poly(ethyleneglycol)-N6-(2-aminoethyl)-NADP+
2-oxoglutarate + NH3 + poly(ethyleneglycol)-N6-(2-aminoethyl)-NADPH + H+
-
-
-
-
r
L-glutamate + H2O + polyethylenglycol-N6-(2-aminoethyl)-NAD(P)+
2-oxoglutarate + NH3 + polyethylenglycol-N6-(2-aminoethyl)-NAD(P)H
-
-
-
?
norvaline + H2O + NADP+
2-oxovalerate + NH3 + NADPH
-
activity is 20% of that observed in the presence of 2-oxoglutarate and L-glutamate
-
-
r
valine + H2O + NADP+
2-oxovalerate + NH3 + NADPH
-
3% of activity with L-glutamate
-
?
2-oxoglutarate + NADH + NH3
L-glutamate + NAD+ + H2O
-
-
-
-
?
2-oxoglutarate + NADH + NH3
L-glutamate + NAD+ + H2O
-
no activity with NAD+ as cofactor
-
-
ir
2-oxoglutarate + NH3 + NADH
L-glutamate + H2O + NAD+
-
specific activity in the presence of NADH is about 30% of that with NADPH
-
-
r
2-oxoglutarate + NH3 + NADH + H+
L-glutamate + H2O + NAD+
-
NH4Cl used in enzyme assay
-
-
r
2-oxoglutarate + NH3 + NADH + H+
L-glutamate + H2O + NAD+
-
-
-
-
r
2-oxoglutarate + NH3 + NADH + H+
L-glutamate + H2O + NAD+
-
NH4Cl used in enzyme assay
-
-
r
2-oxoglutarate + NH3 + NADH + H+
L-glutamate + H2O + NAD+
-
NH4Cl used in enzyme assay
-
-
r
2-oxoglutarate + NH3 + NADH + H+
L-glutamate + H2O + NAD+
-
NH4Cl used in enzyme assay
-
-
r
2-oxoglutarate + NH3 + NADH + H+
L-glutamate + H2O + NAD+
-
-
-
-
r
2-oxoglutarate + NH3 + NADH + H+
L-glutamate + H2O + NAD+
-
NH4Cl used in enzyme assay
-
-
r
2-oxoglutarate + NH3 + NADH + H+
L-glutamate + H2O + NAD+
-
NH4Cl used in enzyme assay
-
-
r
2-oxoglutarate + NH3 + NADH + H+
L-glutamate + H2O + NAD+
-
NH4Cl used in enzyme assay
-
-
r
2-oxoglutarate + NH3 + NADH + H+
L-glutamate + H2O + NAD+
-
-
-
r
2-oxoglutarate + NH3 + NADH + H+
L-glutamate + H2O + NAD+
-
NH4Cl used in enzyme assay
-
-
r
2-oxoglutarate + NH3 + NADPH + H+
L-glutamate + H2O + NADP+
-
NH4Cl used in enzyme assay
-
-
r
2-oxoglutarate + NH3 + NADPH + H+
L-glutamate + H2O + NADP+
-
NH4Cl used in enzyme assay
-
-
r
2-oxoglutarate + NH3 + NADPH + H+
L-glutamate + H2O + NADP+
-
-
-
?
2-oxoglutarate + NH3 + NADPH + H+
L-glutamate + H2O + NADP+
-
-
-
r
alanine + H2O + NAD(P)+
pyruvate + NH3 + NAD(P)H
-
very low activity
-
-
?
alanine + H2O + NAD(P)+
pyruvate + NH3 + NAD(P)H
-
very low activity
-
-
?
alanine + H2O + NAD(P)+
pyruvate + NH3 + NAD(P)H
-
very low activity
-
-
?
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
highly specific for 2-oxoglutarate and glutamate
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
enzyme for the main route for ammonia assimilation at low concentrations of ammonia
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
very low activity with: leucine, alpha-aminobutyrate, valine, isoleucine and methionine
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
rate of glutamate synthesis is several-fold higher than the rate for the reverse reaction
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
highly specific for 2-oxoglutarate and glutamate
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-
-
?
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H + H+
-
-
-
-
?
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H + H+
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H + H+
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H + H+
-
-
-
-
?
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H + H+
-
-
-
r
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H + H+
-
-
-
r
L-glutamate + H2O + NAD+
2-oxoglutarate + NH3 + NADH + H+
-
-
-
r
L-glutamate + H2O + NAD+
2-oxoglutarate + NH3 + NADH + H+
-
-
-
-
r
L-glutamate + H2O + NAD+
2-oxoglutarate + NH3 + NADH + H+
-
-
-
r
L-glutamate + H2O + NAD+
2-oxoglutarate + NH3 + NADH + H+
-
-
-
-
r
L-glutamate + H2O + NAD+
2-oxoglutarate + NH3 + NADH + H+
-
-
-
r
L-glutamate + H2O + NADP+
2-oxoglutarate + NH3 + NADPH + H+
-
-
-
r
L-glutamate + H2O + NADP+
2-oxoglutarate + NH3 + NADPH + H+
-
-
-
r
L-glutamate + H2O + NADP+
2-oxoglutarate + NH3 + NADPH + H+
-
-
-
?
L-glutamate + H2O + NADP+
2-oxoglutarate + NH3 + NADPH + H+
-
-
-
-
r
L-glutamate + H2O + NADP+
2-oxoglutarate + NH3 + NADPH + H+
-
-
-
?
L-glutamate + H2O + NADP+
2-oxoglutarate + NH3 + NADPH + H+
-
-
-
?
L-glutamate + H2O + NADP+
2-oxoglutarate + NH3 + NADPH + H+
-
-
-
r
additional information
?
-
-
the similarity in relative activation when both cofactors are present, combined with consistently greater GDH product formation from equimolar NADH than with NADPH, does not support the idea that there is a preferential utilization of NADPH by bovine GDH
-
-
?
additional information
?
-
-
the glutamate formation activity is 3fold higher than the glutamate deamination activity
-
-
?
additional information
?
-
in vitro, the thermodynamic equilibrium of mammalian GDH favors glutamate synthesis. Because the GDH-catalyzed reaction is reversible, its direction is expected to depend on the concentration of the substrates and the affinity of the enzyme (Km value) for these substrates. In addition to substrate concentrations, the GDH catalysis is affected by the pH, the ionic strength and the composition of the buffer
-
-
-
additional information
?
-
in vitro, the thermodynamic equilibrium of mammalian GDH favors glutamate synthesis. Because the GDH-catalyzed reaction is reversible, its direction is expected to depend on the concentration of the substrates and the affinity of the enzyme (Km value) for these substrates. In addition to substrate concentrations, the GDH catalysis is affected by the pH, the ionic strength and the composition of the buffer
-
-
-
additional information
?
-
-
in vitro, the thermodynamic equilibrium of mammalian GDH favors glutamate synthesis. Because the GDH-catalyzed reaction is reversible, its direction is expected to depend on the concentration of the substrates and the affinity of the enzyme (Km value) for these substrates. In addition to substrate concentrations, the GDH catalysis is affected by the pH, the ionic strength and the composition of the buffer
-
-
-
additional information
?
-
the binding affinity of L-glutamate is lower than that of 2-oxoglutarate. Hence, L-glutamate has a weaker affinity than 2-oxoglutarate in binding to the active site of GDH. A comparison of binding site in 2-oxoglutarate and L-glutamate indicate three residues which include Arg401, Trp88, and Arg12 that have important role in interaction between these ligands with active site of GDH
-
-
-
additional information
?
-
-
no activity in the amination reaction direction with NADH or NADPH, 2-oxoglutarate and ammonia
-
-
?
