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1.4.1.23: valine dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about valine dehydrogenase (NAD+), go to the full flat file.

Reaction

L-valine
+
H2O
+
NAD+
=
3-methyl-2-oxobutanoate
+
NH3
+
NADH
+
H+

Synonyms

VDH, VDH2

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.23 valine dehydrogenase (NAD+)

Storage Stability

Storage Stability on EC 1.4.1.23 - valine dehydrogenase (NAD+)

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, pH 7.4, presence of 2-mercaptoethanol and 30% glycerol, loss of 90% of activity with 24 h
-
-25°C, 0.2 M Tris-HCl buffer, pH 7.4, 2 months, no loss of activity
-
-80°C, presence of 20% glycerol, stable for at least 2 months
-
4°C, 70% (NH4)2SO4 suspension, 3 weeks, no loss of activity
-
4°C, pH 7.4, presence of 2-mercaptoethanol and 10% glycerol, stable for 48 h
-