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1.4.1.21: aspartate dehydrogenase

This is an abbreviated version!
For detailed information about aspartate dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.21

Reaction

2-iminosuccinate
+
H2O
=
oxaloacetate
+
NH3

Synonyms

AspDH, L-aspartate dehydrogenase, L-aspartate:NAD(P)+ oxidoreductase (deaminating), L-aspDH, NAD-dependent aspartate dehydrogenase, NADH2-dependent aspartate dehydrogenase, NADP+-dependent aspartate dehydrogenase, nadX

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.21 aspartate dehydrogenase

Cloned

Cloned on EC 1.4.1.21 - aspartate dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AspDH gene cluster, gene AspDH expression in Escherichia coli strain BL21(DE3), quantitative real-time PCR expression analysis
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli
expressed in Escherichia coli
expression in Escherichia coli
expression of N-terminally His-tagged and GFP-tagged enzyme, using the flexible GGSGG linker, in Escherichia coli. The recombinant tagged aspartate dehydrogenase functions as the biorecognition element, and aspartate-induced conformational change is converted to a fluorescence signal by GFP, method, overview
-
gene KPN_03362, DNA and amino acid sequence determination and analysis
-
gene nadX, phylogenetic analysis
gene nadX, the gene forms an operon with the NAD biosynthesis genes nadA and nadC
-
ligated into the expression vector pET11a, expression in Escherichia coli strain BL21(DE3)
-
ORF PA3505, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21 (DE3)
-