1.4.1.20: phenylalanine dehydrogenase
This is an abbreviated version!
For detailed information about phenylalanine dehydrogenase, go to the full flat file.
Reaction
Synonyms
dehydrogenase, phenylalanine, L-PheDH, L-phenylalanine dehydrogenase, L-phenylalanine:NAD+ oxidoreductase, deaminating, PDH, PHD, PheDH, phenylalanine dehydrogenase, recombinant PheDH, recombinant phenylalanine dehydrogenase
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Cloned
Cloned on EC 1.4.1.20 - phenylalanine dehydrogenase
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chimeric enzyme consisting of the N-terminal domain of Thermoactinomyces intermedius phenylalanine dehydrogenase, containing the substrate-binding region and the C-terminal domain of leucine dehydrogenase from Bacillus stearothermophilus containing the NAD+-binding region, expression in Escherichia coli
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expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
gene pdh, DNA and amino acid sequence determination and analysis, overexpression of His-tagged enzyme in Escherchia coli, method optimization, overview
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the modified enzyme (contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus) is expressed in Pichia pastoris strain SMD1168 or Escherichia coli strain SC 16496
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wild-type and mutant enzymes L307V, G124A and G124A/L307V expressed in Escherichia coli
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