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1.4.1.20: phenylalanine dehydrogenase

This is an abbreviated version!
For detailed information about phenylalanine dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.20

Reaction

L-phenylalanine
+
H2O
+
NAD+
=
phenylpyruvate
+
NH3
+
NADH
+
H+

Synonyms

dehydrogenase, phenylalanine, L-PheDH, L-phenylalanine dehydrogenase, L-phenylalanine:NAD+ oxidoreductase, deaminating, PDH, PHD, PheDH, phenylalanine dehydrogenase, recombinant PheDH, recombinant phenylalanine dehydrogenase

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.20 phenylalanine dehydrogenase

Cloned

Cloned on EC 1.4.1.20 - phenylalanine dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
chimeric enzyme consisting of the N-terminal domain of Thermoactinomyces intermedius phenylalanine dehydrogenase, containing the substrate-binding region and the C-terminal domain of leucine dehydrogenase from Bacillus stearothermophilus containing the NAD+-binding region, expression in Escherichia coli
-
espressed as recombinant protein in Escherichia coli strain BL21 (DE3)
-
expressed at high levels in Escherichia coli
-
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli BL21 (DE3)/pBBNH cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)/pBBNH cells
-
expressed in Escherichia coli BL21/pBBNH cells
-
expressed in Escherichia coli via plasmids
-
expression in Bacillus subtilis
-
expression in Escherichia coli
gene pdh, DNA and amino acid sequence determination and analysis, overexpression of His-tagged enzyme in Escherchia coli, method optimization, overview
-
overexpression in Escherichia coli
-
the modified enzyme (contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus) is expressed in Pichia pastoris strain SMD1168 or Escherichia coli strain SC 16496
-
wild-type and mutant enzymes L307V, G124A and G124A/L307V expressed in Escherichia coli
-