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1.4.1.16: diaminopimelate dehydrogenase

This is an abbreviated version!
For detailed information about diaminopimelate dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.16

Reaction

meso-2,6-diaminoheptanedioate
+
H2O
 +
NADP+
=
L-2-amino-6-oxoheptanedioate
 +
NH3
 +
NADPH
 +
H+

Synonyms

BAB07799, BF3690, DAPDH, DDH, meso-2,6-diaminopimelate dehydrogenase, meso-alpha,epsilon-diaminopimelate dehydrogenase, meso-DAPDH, meso-diaminopimelate dehydrogenase, NADP+-dependent meso-diaminopimelate dehydrogenase, Sth1425, Theth_1310

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.16 diaminopimelate dehydrogenase

Cloned

Cloned on EC 1.4.1.16 - diaminopimelate dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain Rosetta (DE3)
-
DNA and amino acid sequence determmination and analysis, expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), equally as a soluble protein and in inclusion bodies
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) pLysS cells
expressed in Escherichia coli Rosetta (DE3) cells
expression in Escherichia coli
expression in Escherichia coli and Corynebacterim glutamicum
-
gene ddh, expression of wild-type enzyme and mutant Q154L/D158G/T173I/R199M/H249N in Escherichia coli strain Rosetta (DE3)
gene ddh, overexpression in Corynebacterium glutamicum, co-expression with other enzyme of the metabolic pathway for cadaverine, i.e. N-acetyl-diaminopentane, production, overview
-
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)