1.4.1.13: glutamate synthase (NADPH)
This is an abbreviated version!
For detailed information about glutamate synthase (NADPH), go to the full flat file.
Word Map on EC 1.4.1.13
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1.4.1.13
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nitrate
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ammonia
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seedling
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no3
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ferredoxin-dependent
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chlorophyll
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shoot
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ferredoxin
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6.3.1.2
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iron-sulfur
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nadh-dependent
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azaserine
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nitrogenase
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1.4.7.1
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azospirillum
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assimilatory
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alpha-ketoglutarate
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brasilense
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amidotransferase
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glutaminase
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hydroponic
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photorespiratory
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photorespiration
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15n-labeled
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n2-fixing
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molecular biology
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nadp-glutamate
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heterocysts
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glutamine-dependent
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ureides
- 1.4.1.13
- nitrate
- ammonia
- seedling
- no3
-
ferredoxin-dependent
- chlorophyll
- shoot
- ferredoxin
-
6.3.1.2
-
iron-sulfur
-
nadh-dependent
- azaserine
- nitrogenase
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1.4.7.1
- azospirillum
-
assimilatory
- alpha-ketoglutarate
- brasilense
-
amidotransferase
- glutaminase
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hydroponic
-
photorespiratory
-
photorespiration
-
15n-labeled
-
n2-fixing
- molecular biology
-
nadp-glutamate
- heterocysts
-
glutamine-dependent
-
ureides
Reaction
Synonyms
EC 2.6.1.53, EhNO1, EhNO2, gltA, gltB, GltB1, GltB2, gltD, GltS, glutamate synthetase (NADP), glutamine amide-2-oxoglutarate aminotransferase (oxidoreductase, NADP), glutamine-ketoglutaric aminotransferase, GOGAT, L-glutamate synthase, L-glutamate synthetase, L-glutamine:2-oxoglutarate aminotransferase, NADPH oxidizing, NADPH-dependent glutamate synthase, NADPH-GltS, NADPH-glutamate synthase, NADPH-GOGAT, NADPH-linked glutamate synthase, pGLTY1, pGLTY2, pGLTZ, PH0876, PH1873, synthase, glutamate (reduced nicotinamide adenine dinucleotide phosphate)
ECTree
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Purification
Purification on EC 1.4.1.13 - glutamate synthase (NADPH)
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of the alpha subunit, using column chromatography on Q-Sepharose and Ultrogel AcA34
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of the G298A-beta subunit, using column chromatography on Reactive Red or Amicon Red resins and gel filtration on Ultrogel AcA 34
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of the recombinant enzyme beta subunit, using ammonium sulfate fractionation, affinity chromatography on Reactive Red, ultrafiltration and column chromatography on Ultrogel AcA 54
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of the recombinant enzyme from overproducing Escherichia coli cells, using ion exchange chromatography on Q-Sepharose, gel filtration on Sephacryl S-300 and affinity chromatography on 2',5' ADP-Sepharose 4B colum
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partial, using ultrasonic oscillation, ammonium sulfate precipitation, column chromatography on Sephacryl S300 and DE-52 cellulose
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using ammonium sulfate fractionation, column chromatography on DEAE-cellulose and Blue-Sepharose
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using ammonium sulfate fractionation, column chromatography on DEAE-cellulose and Sephadex G-200
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using ammonium sulfate fractionation, column chromatography on DEAE-cellulose, Sepharose 6B, hydroxyapatite, glutamate dehydrogenase antibody affinity chromatography and column chromatography on Sephacryl S-200
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using ammonium sulfate fractionation, coulmn chromatography on DEAE-cellulose, hydroxyapatite, Sepharose 6B and Sephadex G-200
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using ammonium sulfate fractionation, gel filtration on Sephacryl S-300 and affinity chromatography on NADPH-Sepharose
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using ammonium sulfate precipitation and column chromatography on DEAE-Sepharose, 2',5'-ADP-Sepharose and Sephacryl S-300
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using heat treatment, ammonium sulfate precipitation, column chromatography on DEAE-Trisacryl, gel filtration and affinity chromatogray
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using heat treatment, anion-exchange column chromatography on HiTrap Q and cation exchange column chromatography on Resources S
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using protamine sulfate precipitation, ammonium sulfate precipitation, column chromatography on DE52, DEAE-Sephadex, hydroxyapatite, phenyl-Sepharose CL-4B and Bio-Gel filtration
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using sonication, heat treatment, protamine sulfate precipitation, ammonium sulfate precipitation, gel filtration on G-50, column chromatography on DEAE-Sephadex and gel filtration on Sephadex G-200 and Sepharose 6B
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using streptomycin sulfate treatment, ammonium sulfate fractionation, heat treatment, agarose gel filtration and DEAE-cellulose column chromatography
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using streptomycin sulfate treatment, ammonium sulfate precipitation, column chromatography on DEAE-Sephacel, Bio-Gel A 1.5 m, Red Sepharose CL6B and Sephadex G-25
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using streptomycin sulfate treatment, ammonium sulfate precipitation, column chromatography on Ultrogel AcA 22 and DEAE-Sephadex A-50, ultrafiltration with an Amicon PM 30 membrane and chromatography on hydroxyapatite column
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using sulfate precipitation, gel filtration and column chromatography on 2',5'-ADP-Sepharose
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