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1.4.1.13: glutamate synthase (NADPH)

This is an abbreviated version!
For detailed information about glutamate synthase (NADPH), go to the full flat file.

Word Map on EC 1.4.1.13

Reaction

2 L-glutamate +

NADP+
=
L-glutamine
 +
2-oxoglutarate
 +
NADPH
 +
H+

Synonyms

EC 2.6.1.53, EhNO1, EhNO2, gltA, gltB, GltB1, GltB2, gltD, GltS, glutamate synthetase (NADP), glutamine amide-2-oxoglutarate aminotransferase (oxidoreductase, NADP), glutamine-ketoglutaric aminotransferase, GOGAT, L-glutamate synthase, L-glutamate synthetase, L-glutamine:2-oxoglutarate aminotransferase, NADPH oxidizing, NADPH-dependent glutamate synthase, NADPH-GltS, NADPH-glutamate synthase, NADPH-GOGAT, NADPH-linked glutamate synthase, pGLTY1, pGLTY2, pGLTZ, PH0876, PH1873, synthase, glutamate (reduced nicotinamide adenine dinucleotide phosphate)

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.13 glutamate synthase (NADPH)

Cloned

Cloned on EC 1.4.1.13 - glutamate synthase (NADPH)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a construct with the His-tag extension is cloned and expressed in Escherichia coli as soluble protein
DNA and amino acid sequence determination and analysis, sequence comparison, quantitative real-time PCR expression analysis, recombinant expression in Escherichia coli strain BL21(DE3)
expressed in Escherichia coli BL21 (DE3) cells
expressed in native form (GLTY1) as well as with an added His-tag (GLTY2). A construct with the His-tag extension is cloned and expressed in Escherichia coli as soluble protein
expression in GOGAT large subunit-deficient Escherichia coli JRG72 mutants
expression of the holoenzyme in Escherichia coli
-
gene PH0876, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain Rosetta (DE3)
gene PH1873, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain Rosetta (DE3)
overproduction of the holoenzyme and of the alpha subunit in Escherichia coli
-
production of the alpha and beta subunits in Escherichia coli
-
production of the beta subunit in Escherichia coli
-
production of the G298A-beta subunit in Escherichia coli
-
the gltA gene encoding the enzyme overexpressed in Escherichia coli
-