Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.4.1.1: alanine dehydrogenase

This is an abbreviated version!
For detailed information about alanine dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.1

Reaction

L-alanine
+
H2O
 +
NAD+
=
pyruvate
 +
NH3
 +
NADH
 +
H+

Synonyms

(S)alanine dehydrogenase, (S)alanine:NAD oxidoreductase, 40 kDa antigen, ADH, AF1665, ALADH, alanine dehydrogenase, alanine oxidative deaminase, alanine oxidoreductase, ALD, AldA, alpha-alanine dehydrogenase, ApalaDH, AsAlaDH, CQA66_00465, dehydrogenase, alanine, glyoxylate reductive aminase, GxrA, HAADH1, HAADH2, L-Ala dehydrogenase, L-AlaDH, L-AlaDH-Bs, L-alanine dehydrogenase, MtbALD, NAD(H)-dependent L-alanine dehydrogenase, NAD+ dependent amino acid dehydrogenase, NAD+-dependent alanine dehydrogenase, NAD-dependent alanine dehydrogenase, NAD-linked alanine dehydrogenase, NADH-dependent alanine dehydrogenase, OF4Ald, PlaAlaDH, PvRA, pyruvate reductive aminase, Rv2780, ScALD, SCO1773, SheAlaDH, TB43

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.1 alanine dehydrogenase

Storage Stability

Storage Stability on EC 1.4.1.1 - alanine dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.2 M Tris/HCl buffer, pH 7.4, 2 months, stable
-
-20°C, in presence of 50% glycerol, stable for up to 5 months
-
-20°C, pH 7.2, more than 5 months
-
-20°C, stable for at least 1 month
-4°C, 0.2 M Tris/HCl buffer, pH 7.4, 24 h, stable
-
4°C or -20°C, less than 0.2 mg/ml, enzyme loses most of its activity in less than 2 weeks
-
4°C, 0.02 M Tris-HCl buffer, pH 8.4, stable for 1 d, then the activity decreases gradually till the sixth day at which complete loss of activity occurs
-
4°C, 0.15 M KCl, stable for more than 2 months
-
4°C, 60% saturation with ammonium sulfate, pH 7.2, stable for over 6 months
-
4°C, 75% saturated ammonium sulfate suspension, purified enzyme stable for at least 12 months
-
4°C, 80% saturated ammonium sulfate suspension, pH 7-8, 2 years, stable
-
4°C, PEG-succinimide-immobilized or PEG-p-nitrophenylchloroformate-immobilized AlaDH in 50 mM phosphate buffer, 15 days, no loss of activity
-
4°C, pH 6-8, 50 mM phosphate buffer, stable for at least 3 days
-
4°C, stable for many months
-
4°C, stable up to 1 week without degradation
purified native enzyme, significant loss in enzyme activity at 25°C and 35°C on the 15th day and almost no activity on the 120th day, but the enzyme retains most of the activity in the presence of EDTA, 2-ME and DTT