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1.4.1.1: alanine dehydrogenase

This is an abbreviated version!
For detailed information about alanine dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.1

Reaction

L-alanine
+
H2O
+
NAD+
=
pyruvate
+
NH3
+
NADH
+
H+

Synonyms

(S)alanine dehydrogenase, (S)alanine:NAD oxidoreductase, 40 kDa antigen, ADH, AF1665, ALADH, alanine dehydrogenase, alanine oxidative deaminase, alanine oxidoreductase, ALD, AldA, alpha-alanine dehydrogenase, ApalaDH, AsAlaDH, CQA66_00465, dehydrogenase, alanine, glyoxylate reductive aminase, GxrA, HAADH1, HAADH2, L-Ala dehydrogenase, L-AlaDH, L-AlaDH-Bs, L-alanine dehydrogenase, MtbALD, NAD(H)-dependent L-alanine dehydrogenase, NAD+ dependent amino acid dehydrogenase, NAD+-dependent alanine dehydrogenase, NAD-dependent alanine dehydrogenase, NAD-linked alanine dehydrogenase, NADH-dependent alanine dehydrogenase, OF4Ald, PlaAlaDH, PvRA, pyruvate reductive aminase, Rv2780, ScALD, SCO1773, SheAlaDH, TB43

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.1 alanine dehydrogenase

Purification

Purification on EC 1.4.1.1 - alanine dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene cloned and expressed in Escherichia coli
-
native enzyme
-
native enzyme by ammonium sulfate fractionation, dialysis, anion exchange chromatography, again dialysis, and gel filtration
Ni-NTA resin column chromatography
-
partial
recombinant enzyme
recombinant GST-tagged enzyme from Escherichia coli strain JM109 by glutathione affinity chrmatography
-
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and dialysis
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain ER2566 by nickel affinity chromatography, native enzyme by anion exchange and hydrophobic interaction chromatography, followed by ultrafiltration
-
recombinant His-tagged isozyme HAADH1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged isozyme HAADH2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Alkalihalophilus pseudofirmus
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
the GST-AlaDH fusion protein is released by cleaving with PreScission protease
using affinity chromatography