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1.4.1.1: alanine dehydrogenase

This is an abbreviated version!
For detailed information about alanine dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.1

Reaction

L-alanine
+
H2O
 +
NAD+
=
pyruvate
 +
NH3
 +
NADH
 +
H+

Synonyms

(S)alanine dehydrogenase, (S)alanine:NAD oxidoreductase, 40 kDa antigen, ADH, AF1665, ALADH, alanine dehydrogenase, alanine oxidative deaminase, alanine oxidoreductase, ALD, AldA, alpha-alanine dehydrogenase, ApalaDH, AsAlaDH, CQA66_00465, dehydrogenase, alanine, glyoxylate reductive aminase, GxrA, HAADH1, HAADH2, L-Ala dehydrogenase, L-AlaDH, L-AlaDH-Bs, L-alanine dehydrogenase, MtbALD, NAD(H)-dependent L-alanine dehydrogenase, NAD+ dependent amino acid dehydrogenase, NAD+-dependent alanine dehydrogenase, NAD-dependent alanine dehydrogenase, NAD-linked alanine dehydrogenase, NADH-dependent alanine dehydrogenase, OF4Ald, PlaAlaDH, PvRA, pyruvate reductive aminase, Rv2780, ScALD, SCO1773, SheAlaDH, TB43

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.1 alanine dehydrogenase

Cloned

Cloned on EC 1.4.1.1 - alanine dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AlaDH gene is cloned into a pGEX6p-1 and expressed in Escherichia coli JM109. AlaDH gene is then cloned intoa vector pNZ8148 to generate a vector pNZ8148/ald. The same AlaDH gene is placed downstream of the lactate dehydrogenase (ldh) promoter from Streptococcus thermophilus to generate pNZ273/ldhp/AlaDH. The pNZ8148/AlaDH and pNZ273/ldhp/AlaDH are introduced separately in Lactococcus lactis NZ9000. As a result of over-expressed AlaDH, the production of alanine detected by HPLC in Lactococcus lactis NZ9000 carrying pNZ273/ldhp/ald reaches 52 mg/ml, an approximately 26fold increase compared to the parent strain Lactococcus lactis NZ9000, but not in Lactococcus lactis NZ9000 carrying pNZ8148/ALaDH
cloned and expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli strain TBLA-1, an H+-ATPase- and lactate dehydrogenase-defective mutant of Escherichia coli derived from the pyruvate producing K-12 strain W1485lip2
-
expressed in Escherichia coli strain W (ATCC 9637)
expression in Escherichia coli
expression in Escherichia coli JM109
-
expression in Escherichia coli TG1 with a vector plasmid, pUC118
expression of N-terminally GFP-fused enzyme in Anabaena sp. strain CSR30
-
gene AlaDH, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene AlaDH, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene ald, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
Alkalihalophilus pseudofirmus
gene ald, expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene ald, functional heterologous expression in Escherichia coli strain JM109, as GST-tagged protein, and in Lactococcus lactis strain NZ9000
-
gene ald, genetic organization of the aldR-ald loci and their flanking genes
gene ald, phylogenetic tree, recombinant expression of His-tagged isozyme HAADH1 in Escherichia coli strain BL21(DE3)
gene ald, quantitative and qualitative RT-PCR enzyme expression analysis
gene ald, quantitative real-time PCR expression analysis, expression in Escherichia coli strain ER2566
-
gene ApalaDH, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21, subcloning in Escherichia coli strain DH5alpha
gene CQA66_00465, phylogenetic tree, recombinant expression of His-tagged isozyme HAADH2 in Escherichia coli strain BL21(DE3)
gene SCO1773, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli
host vector system for high-level expression in Escherichia coli
-
into vector pJEX58, resulting plasmid pGS3 expressed in Mycobacterium bovis BCG Pasteur
-
overexpression in Escherichia coli