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1.3.99.4: 3-oxosteroid 1-dehydrogenase

This is an abbreviated version!
For detailed information about 3-oxosteroid 1-dehydrogenase, go to the full flat file.

Word Map on EC 1.3.99.4

Reaction

a 3-oxosteroid
+
acceptor
=
a 3-oxo-DELTA1-steroid
+
reduced acceptor

Synonyms

1-ene-dehydrogenase, 3-keto-5alpha-steroid DELTA(1)-dehydrogenase, 3-ketosteroid 1(2)-dehydrogenase, 3-ketosteroid delta1-dehydrogenase, 3-ketosteroid-1-en-dehydrogenase, 3-ketosteroid-D1-dehydrogenase, 3-ketosteroid-DELTA1-dehydrogenase, 3-ketosteroid-DELTA1-dehydrogenases, 3-oxosteroid D1-dehydrogenase, 3-oxosteroid Delta1-dehydrogenase, 3-oxosteroid:(2,6-dichlorphenolindophenol) DELTA1-oxidoreductase, 3-oxosteroid:(2,6-dichlorphenolindophenol)D1-oxidoreductase, 4-en-3-oxosteroid:(acceptor)-1-en-oxido-reductase, 4-ene-3-oxosteroid: (acceptor)-1-ene-oxoreductase, 4-ene-3-oxosteroid:(acceptor)l-ene-oxidoreductase, AcmB, AcmB2, cholest-4-en-3-one DELTA1-dehydrogenase, D1-dehydrogenase, D1-steroid reductase, dehydrogenase, 3-oxo steroid Delta 1-, Delta1-dehydrogenase, DELTA1-KstD, DELTA1-KSTD1, DELTA1-KstDA, DELTA1-KstDR, DELTA1-steroid reductase, ketosteroid DELTA1-dehydrogenase, KsdD, KsdD1, KsdD2, KsdD3, KsdD5, KsdDM, KSDH, KstD, KSTD1, KSTD2, KSTD3, KSTD3 orthologue, KstDF, MnKstD1, MnKstD2, MsKstD1, MsKstD2, MsKstD3, MsKstD4, ReKstD1, Rv3537, steroid-1-dehydrogenase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.99 With unknown physiological acceptors
                1.3.99.4 3-oxosteroid 1-dehydrogenase

Specific Activity

Specific Activity on EC 1.3.99.4 - 3-oxosteroid 1-dehydrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000015
-
intracellular, soluble, recombinant enzyme, in 0.86 M Tris-HCl (pH9.0), 150 mM phenazine methosulfate, at 25°C, using 4-androstene-3,17-dione as substrate
0.000111
-
exracellular, soluble, recombinant enzyme, in 0.86 M Tris-HCl (pH9.0), 150 mM phenazine methosulfate, at 25°C, using 4-androstene-3,17-dione as substrate
0.8
soluble recombinant His-tagged enzyme, cell extract, pH 7.0, 30°C
1.46
-
pH 7.0, 30°C
1.75
-
recombinant enzyme expressed in Bacillus subtilis, pH 7.0, 30°C
109200
-
purified recombinant wild-type enzyme, pH 7.5, 35°C, substrate 17alpha,21-dihydroxyprogesterone 21-acetate
12781.5
pH 8.0, 40°C, purified recombinant enzyme, substrate 6,17alpha-epoxyprogesterone
15700
-
purified recombinant wild-type enzyme, pH 7.0, 30°C, substrate progesterone
1586
purified enzyme, pH 8.0, 40°C, substrate 4-androstene-3,17-dione, conversion rate is 99%
1719.21
pH 8.0, 40°C, purified recombinant enzyme, substrate 9alpha-hydroxy-4-androstene-3,17-dione
23100
-
purified recombinant wild-type enzyme, pH 7.0, 30°C, substrate testosterone
2560.15
pH 8.0, 40°C, purified recombinant enzyme, substrate canrenone
2623.11
pH 8.0, 40°C, purified recombinant enzyme, substrate 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione
40900
-
purified recombinant wild-type enzyme, pH 7.0, 30°C, substrate 4-cholesten-3-one
51300
-
purified recombinant wild-type enzyme, pH 7.5, 35°C, substrate testosterone
51600
-
purified recombinant wild-type enzyme, pH 7.5, 35°C, substrate progesterone
59200
-
purified recombinant wild-type enzyme, pH 7.5, 35°C, substrate cortisone acetate
5975.86
pH 8.0, 40°C, purified recombinant enzyme, substrate androsta-4,9(11)-diene-3,17-dione
6299.29
pH 8.0, 40°C, purified recombinant enzyme, substrate 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate
66200
-
purified recombinant wild-type enzyme, pH 7.0, 30°C, substrate 17-methyl-testosterone
72600
-
purified recombinant wild-type enzyme, pH 7.5, 35°C, substrate 4-androstene-3,17-dione
7800.55
pH 8.0, 40°C, purified recombinant enzyme, substrate 17alpha,21-dihydroxyprogesterone 21-acetate
80600
-
purified recombinant wild-type enzyme, pH 7.0, 30°C, substrate 4-androstene-3,17-dione
81700
-
purified recombinant wild-type enzyme, pH 7.5, 35°C, substrate 17alpha-hydroxyprogesterone
8882.95
pH 8.0, 40°C, purified recombinant enzyme, substrate 4-androstene-3,17-dione
90700
-
purified recombinant wild-type enzyme, pH 7.5, 35°C, substrate 17-methyl-testosterone
91500
-
purified recombinant wild-type enzyme, pH 7.0, 30°C, substrate canrenone
9163.63
pH 8.0, 40°C, purified recombinant enzyme, substrate 17alpha-hydroxypregn-4-ene-3,20-dione
additional information
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