1.3.99.4: 3-oxosteroid 1-dehydrogenase
This is an abbreviated version!
For detailed information about 3-oxosteroid 1-dehydrogenase, go to the full flat file.
Word Map on EC 1.3.99.4
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1.3.99.4
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rhodococcus
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3-ketosteroids
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mycobacterium
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cholesterol
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4-androstene-3,17-dione
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erythropolis
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synthesis
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arthrobacter
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simplex
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isoenzymes
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testosteroni
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rhodochrous
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androst-4-ene-3,17-dione
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bioconversion
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hydrocortisone
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dehydrogenation
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phytosterols
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ruber
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steroid-transforming
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globiformis
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1,4-androstadiene-3,17-dione
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neoaurum
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molecular biology
- 1.3.99.4
- rhodococcus
- 3-ketosteroids
- mycobacterium
- cholesterol
- 4-androstene-3,17-dione
- erythropolis
- synthesis
-
arthrobacter
- simplex
- isoenzymes
- testosteroni
- rhodochrous
- androst-4-ene-3,17-dione
-
bioconversion
- hydrocortisone
-
dehydrogenation
- phytosterols
- ruber
-
steroid-transforming
- globiformis
- 1,4-androstadiene-3,17-dione
- neoaurum
- molecular biology
Reaction
Synonyms
1-ene-dehydrogenase, 3-keto-5alpha-steroid DELTA(1)-dehydrogenase, 3-ketosteroid 1(2)-dehydrogenase, 3-ketosteroid delta1-dehydrogenase, 3-ketosteroid-1-en-dehydrogenase, 3-ketosteroid-D1-dehydrogenase, 3-ketosteroid-DELTA1-dehydrogenase, 3-ketosteroid-DELTA1-dehydrogenases, 3-oxosteroid D1-dehydrogenase, 3-oxosteroid Delta1-dehydrogenase, 3-oxosteroid:(2,6-dichlorphenolindophenol) DELTA1-oxidoreductase, 3-oxosteroid:(2,6-dichlorphenolindophenol)D1-oxidoreductase, 4-en-3-oxosteroid:(acceptor)-1-en-oxido-reductase, 4-ene-3-oxosteroid: (acceptor)-1-ene-oxoreductase, 4-ene-3-oxosteroid:(acceptor)l-ene-oxidoreductase, AcmB, AcmB2, cholest-4-en-3-one DELTA1-dehydrogenase, D1-dehydrogenase, D1-steroid reductase, dehydrogenase, 3-oxo steroid Delta 1-, Delta1-dehydrogenase, DELTA1-KstD, DELTA1-KSTD1, DELTA1-KstDA, DELTA1-KstDR, DELTA1-steroid reductase, ketosteroid DELTA1-dehydrogenase, KsdD, KsdD1, KsdD2, KsdD3, KsdD5, KsdDM, KSDH, KstD, KSTD1, KSTD2, KSTD3, KSTD3 orthologue, KstDF, MnKstD1, MnKstD2, MsKstD1, MsKstD2, MsKstD3, MsKstD4, ReKstD1, Rv3537, steroid-1-dehydrogenase
ECTree
1.3.99.4 3-oxosteroid 1-dehydrogenaseAdvanced search results
results (
results (KM Value
KM Value on EC 1.3.99.4 - 3-oxosteroid 1-dehydrogenase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.0246
(5alpha,17beta)-17-hydroxyandrostan-3-one
pH 7.0, 30°C
0.0064
11-deoxy-17alpha-hydroxycorticosterone
-
i.e. 4-pregnene-17alpha,21-diol-3,20-dione
0.0196
11alpha-hydroxyprogesterone
-
i.e. 4-pregnene-11alpha-ol-3,20-dione, 11beta-hydroxy-4-androstene-3,17-dione, 4-estren-3,17-dione
0.0063
11beta-hydroxytestosterone
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i.e. 4-androstene-11beta,17beta-diol-3-one
0.0305
16,17alpha-epoxyprogesterone
recombinant enzyme, pH 8.0, 40°C
0.1552
17alpha-hydroxyprogesterone
-
pH 7.5, 35°C, recombinant isozyme KsdD3
0.1733
androsta-4,9(11)-diene-3,17-dione
recombinant enzyme, pH 8.0, 40°C
-
0.0385
hydrocorticosterone
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i.e. 4-pregnene-11beta,17alpha,21-triol-3,20-dione
0.075
pregna-4,9(11),16-triene-3,20-dione
pH 8.0, 25°C
-
0.0143
testololactone
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i.e. 17alpha-oxo-D-homo-1,4-androstadiene-3,17-dione
0.0401
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione
pH 8.0, 25°C
-
0.0463
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione
pH 8.0, 30°C
-
0.9158
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione
recombinant enzyme, pH 8.0, 40°C
-
0.0232
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate
pH 8.0, 30°C
-
0.1003
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate
pH 8.0, 25°C
-
0.1919
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate
recombinant enzyme, pH 8.0, 40°C
-
0.064
11beta-cortisol
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.557
11beta-cortisol
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.0885
17-methyl-testosterone
-
pH 7.0, 30°C, recombinant isozyme KsdD3
-
0.1527
17-methyl-testosterone
-
pH 7.5, 35°C, recombinant isozyme KsdD3
-
0.0158
17alpha,21-dihydroxyprogesterone 21-acetate
recombinant enzyme, pH 8.0, 40°C
-
0.1512
17alpha,21-dihydroxyprogesterone 21-acetate
-
pH 7.5, 35°C, recombinant isozyme KsdD3
-
0.0211
17alpha-hydroxypregn-4-ene-3,20-dione
recombinant enzyme, pH 8.0, 40°C
0.0917
17alpha-hydroxypregn-4-ene-3,20-dione
pH 8.0, 25°C
0.3474
19-hydroxy-4-androstene-3,17-dione
pH 7.0, 30°C
0.3688
19-hydroxy-4-androstene-3,17-dione
pH 7.0, 30°C
0.0373
2,6-dichlorophenol-indophenol
pH 6.5, 30°C, recombinant enzyme, with 4-cholest-3-one
0.2235
2,6-dichlorophenol-indophenol
pH 6.5, 30°C, recombinant enzyme, with 4-androstene-3,17-dione
0.0178
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone
pH 8.0, 30°C
-
0.0373
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone
pH 8.0, 25°C
-
0.0193
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione
pH 8.0, 30°C
-
0.0985
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione
pH 8.0, 25°C
-
0.026
23,24-bis-nor-5alpha-cholestan-3-one acid
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.074
23,24-bis-nor-5alpha-cholestan-3-one acid
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.2102
4-androsten-3-one-5-ene-17-carboxylic acid
pH 8.0, 25°C
-
0.2462
4-androsten-3-one-5-ene-17-carboxylic acid
pH 8.0, 30°C
-
0.011
4-androstene-17beta-ol-3-one
pH 7.0, 30°C
0.082
4-androstene-17beta-ol-3-one
pH 7.0, 30°C
0.085
4-androstene-17beta-ol-3-one
pH 7.0, 30°C
0.005
4-androstene-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.104
4-androstene-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.1264
4-androstene-3,17-dione
-
pH 7.0, 30°C, recombinant isozyme KsdD3
0.1385
4-androstene-3,17-dione
pH 8.0, 25°C
0.1434
4-androstene-3,17-dione
recombinant enzyme, pH 8.0, 40°C
0.5292
4-androstene-3,17-dione
pH 6.5, 30°C, recombinant enzyme
0.0237
4-Cholesten-3-one
pH 6.5, 30°C, recombinant enzyme
0.0382
4-pregnen-3-one-20beta-carboxylic acid
pH 7.0, 30°C
0.0761
4-pregnen-3-one-20beta-carboxylic acid
pH 7.0, 30°C
0.006
5alpha-androstan-3-one-17beta-ol
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.036
5alpha-androstan-3-one-17beta-ol
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.07
5alpha-androstan-3-one-17beta-ol
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.166
5alpha-androstan-3-one-17beta-ol
-
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.035
5alpha-androstane-17beta-ol-3-one
pH 7.0, 30°C
0.0379
5alpha-androstane-17beta-ol-3-one
pH 7.0, 30°C
0.013
5alpha-androstane-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.033
5alpha-androstane-3,17-dione
-
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.036
5alpha-androstane-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.059
5alpha-androstane-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.007
5alpha-pregnane-3,20-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.014
5alpha-pregnane-3,20-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.021
5alpha-pregnane-3,20-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.0221
9alpha-hydroxy-4-androstene-3,17-dione
pH 7.0, 30°C
0.025
9alpha-hydroxy-4-androstene-3,17-dione
pH 7.0, 30°C
0.0369
9alpha-hydroxy-4-androstene-3,17-dione
pH 8.0, 25°C
0.045
9alpha-hydroxy-4-androstene-3,17-dione
pH 7.0, 30°C
0.058
9alpha-hydroxy-4-androstene-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.105
9alpha-hydroxy-4-androstene-3,17-dione
pH 7.0, 30°C
0.119
9alpha-hydroxy-4-androstene-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.5432
9alpha-hydroxy-4-androstene-3,17-dione
pH 7.0, 30°C
0.8327
9alpha-hydroxy-4-androstene-3,17-dione
recombinant enzyme, pH 8.0, 40°C
0.2121
androst-4,9(11)-diene-3,17-dione
pH 8.0, 25°C
-
0.003
progesterone
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.018
progesterone
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.029
progesterone
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.065
progesterone
-
apparent value, in 50 mM Tris (pH 7.4), at 30°C
additional information
additional information
Michaelis-Menten kinetics
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additional information
additional information
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Michaelis-Menten kinetics
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additional information
additional information
steady-state kinetics, kinetic model
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additional information
additional information
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steady-state kinetics, kinetic model
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additional information
additional information
steady-state kinetics, kinetic model
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additional information
additional information
steady-state kinetics, kinetic model
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additional information
additional information
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steady-state kinetics, kinetic model
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