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1.3.99.29: phytoene desaturase (zeta-carotene-forming)

This is an abbreviated version!
For detailed information about phytoene desaturase (zeta-carotene-forming), go to the full flat file.

Word Map on EC 1.3.99.29

Reaction

15-cis-phytoene
+ 2 acceptor =
all-trans-zeta-carotene
+ 2 reduced acceptor

Synonyms

4-step phytoene desaturase, bacterial-type phytoene desaturase, crtI-type phytoene desaturase, Crtla, glr0867, PDS, phytoene desaturase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.99 With unknown physiological acceptors
                1.3.99.29 phytoene desaturase (zeta-carotene-forming)

Engineering

Engineering on EC 1.3.99.29 - phytoene desaturase (zeta-carotene-forming)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N144D
E143K
naturally occcuring mutation, mutation pds1-1
L505F
site-directed mutagenesis, the mutation enhances enzyme desaturation activity by 29% compared to the wild-type enzyme, L505F exhibits a 27.7fold higher resistance to the herbicide norflurazon
L64F/E143K
site-directed mutagenesis, mutation pds1-5, with in-frame deletion of PSY residues 7-14
L64P
site-directed mutagenesis, mutation pds1-4, with in-frame deletion of PSY residues 7-14
L64P/E143K
site-directed mutagenesis, mutation pds1-2, with in-frame deletion of PSY residues 7-14
L64P/K90M/E143K
site-directed mutagenesis, mutation pds1-6, with in-frame deletion of PSY residues 7-14
E143K
-
naturally occcuring mutation, mutation pds1-1
-
L64F/E143K
-
site-directed mutagenesis, mutation pds1-5, with in-frame deletion of PSY residues 7-14
-
L64P
-
site-directed mutagenesis, mutation pds1-4, with in-frame deletion of PSY residues 7-14
-
L64P/E143K
-
site-directed mutagenesis, mutation pds1-2, with in-frame deletion of PSY residues 7-14
-
L505F
-
site-directed mutagenesis, the mutation enhances enzyme desaturation activity by 29% compared to the wild-type enzyme, L505F exhibits a 27.7fold higher resistance to the herbicide norflurazon
-
L516F
naturally occuring mutant strain E17, the mutant enzyme is insensitive, with 31fold higher resistane compared to the wild-tpe enzyme, to inhibition by norflurazon.The mutant also shows higher catalytic efficiency than the wild-type enzyme. Induced by high-light irradiation or glucose, E17 produces 44 or 36% more astaxanthin than the wild-type when cultured in media without norflurazon
L516R
the mutant show reduced enzyme activity compared to the wild-type enzyme
L516F
-
naturally occuring mutant strain E17, the mutant enzyme is insensitive, with 31fold higher resistane compared to the wild-tpe enzyme, to inhibition by norflurazon.The mutant also shows higher catalytic efficiency than the wild-type enzyme. Induced by high-light irradiation or glucose, E17 produces 44 or 36% more astaxanthin than the wild-type when cultured in media without norflurazon
-
L516R
-
the mutant show reduced enzyme activity compared to the wild-type enzyme
-
additional information