1.3.99.29: phytoene desaturase (zeta-carotene-forming)
This is an abbreviated version!
For detailed information about phytoene desaturase (zeta-carotene-forming), go to the full flat file.
Word Map on EC 1.3.99.29
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1.3.99.29
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carotenoid
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lycopene
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desaturation
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neurosporene
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cyanobacterium
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beta-carotene
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desaturases
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gloeobacter
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carotenogenic
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prolycopene
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violaceus
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rhodobacter
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purple
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echinenone
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analysis
- 1.3.99.29
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carotenoid
- lycopene
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desaturation
- neurosporene
- cyanobacterium
- beta-carotene
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desaturases
- gloeobacter
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carotenogenic
- prolycopene
- violaceus
- rhodobacter
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purple
- echinenone
- analysis
Reaction
+ 2 acceptor = + 2 reduced acceptor
Synonyms
4-step phytoene desaturase, bacterial-type phytoene desaturase, crtI-type phytoene desaturase, Crtla, glr0867, PDS, phytoene desaturase
ECTree
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Engineering
Engineering on EC 1.3.99.29 - phytoene desaturase (zeta-carotene-forming)
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N144D
L505F
site-directed mutagenesis, the mutation enhances enzyme desaturation activity by 29% compared to the wild-type enzyme, L505F exhibits a 27.7fold higher resistance to the herbicide norflurazon
L64F/E143K
site-directed mutagenesis, mutation pds1-5, with in-frame deletion of PSY residues 7-14
L64P
site-directed mutagenesis, mutation pds1-4, with in-frame deletion of PSY residues 7-14
L64P/E143K
site-directed mutagenesis, mutation pds1-2, with in-frame deletion of PSY residues 7-14
L64P/K90M/E143K
site-directed mutagenesis, mutation pds1-6, with in-frame deletion of PSY residues 7-14
L64F/E143K
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site-directed mutagenesis, mutation pds1-5, with in-frame deletion of PSY residues 7-14
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L64P
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site-directed mutagenesis, mutation pds1-4, with in-frame deletion of PSY residues 7-14
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L64P/E143K
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site-directed mutagenesis, mutation pds1-2, with in-frame deletion of PSY residues 7-14
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L505F
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site-directed mutagenesis, the mutation enhances enzyme desaturation activity by 29% compared to the wild-type enzyme, L505F exhibits a 27.7fold higher resistance to the herbicide norflurazon
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L516F
naturally occuring mutant strain E17, the mutant enzyme is insensitive, with 31fold higher resistane compared to the wild-tpe enzyme, to inhibition by norflurazon.The mutant also shows higher catalytic efficiency than the wild-type enzyme. Induced by high-light irradiation or glucose, E17 produces 44 or 36% more astaxanthin than the wild-type when cultured in media without norflurazon
L516R
the mutant show reduced enzyme activity compared to the wild-type enzyme
L516F
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naturally occuring mutant strain E17, the mutant enzyme is insensitive, with 31fold higher resistane compared to the wild-tpe enzyme, to inhibition by norflurazon.The mutant also shows higher catalytic efficiency than the wild-type enzyme. Induced by high-light irradiation or glucose, E17 produces 44 or 36% more astaxanthin than the wild-type when cultured in media without norflurazon
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L516R
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the mutant show reduced enzyme activity compared to the wild-type enzyme
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additional information
random and site-directed mutagenesis, inactive mutant
N144D
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random and site-directed mutagenesis, inactive mutant
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possibility of engineering the carotenogenic pathway for improved carotenoid production in microalgae, overview
additional information
the naturally occuring pds1-1 allele shows a mutant with reduced enzyme activity and growth defects, isolation of pds1-2 as an intragenic enhancer of pds1-1, the pds1-2 and pds1-3 allele products completely lack PDS activity, pds1-3 is a null allele derived from DNA insertional mutagenesis. The null mutants accumulate only phytoene and no other carotenoids. All three phytoene-accumulating mutants exhibit lower growth rates and reduced plating efficiency compared to wild-type cells and white phytoene synthase mutants. DNA insertional mutagenesis, phenotypes, detailed overview
additional information
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the naturally occuring pds1-1 allele shows a mutant with reduced enzyme activity and growth defects, isolation of pds1-2 as an intragenic enhancer of pds1-1, the pds1-2 and pds1-3 allele products completely lack PDS activity, pds1-3 is a null allele derived from DNA insertional mutagenesis. The null mutants accumulate only phytoene and no other carotenoids. All three phytoene-accumulating mutants exhibit lower growth rates and reduced plating efficiency compared to wild-type cells and white phytoene synthase mutants. DNA insertional mutagenesis, phenotypes, detailed overview
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additional information
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possibility of engineering the carotenogenic pathway for improved carotenoid production in microalgae, overview
-