1.3.99.28: phytoene desaturase (neurosporene-forming)

This is an abbreviated version!
For detailed information about phytoene desaturase (neurosporene-forming), go to the full flat file.

Reaction

Synonyms

3-step phytoene desaturase, CrtI, PDS, phytoene desaturase

ECTree

     1 Oxidoreductases

         1.3 Acting on the CH-CH group of donors

             1.3.99 With unknown physiological acceptors

                1.3.99.28 phytoene desaturase (neurosporene-forming)

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Results	in table
467	AA Sequence
2	Application
13	Cloned(Commentary)
3	Cofactor
70	General Information
1	IC50 Value
1	Inhibitors
10	KM Value [mM]
1	Localization
3	Molecular Weight [Da]
83	Natural Substrates/ Products (Substrates)
37	Organism
3	Pathway
2	pH Optimum
35	Protein Variants
2	Purification (Commentary)
5	Reaction
35	Reference
1	Specific Activity [micromol/min/mg]
93	Substrates and Products (Substrate)
4	Subunits
63	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]

Substrates Products

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Substrates Products on EC 1.3.99.28 - phytoene desaturase (neurosporene-forming)

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

1,2-epoxyphytoene + acceptor

1,2-epoxyneurosporene + reduced acceptor

Rhodobacter capsulatus

-

-

712316

-

-

?

15-cis-phytoene + 3 acceptor

all-trans-neurosporene + 3 reduced acceptor

4 entries

15-cis-phytoene + acceptor

all-trans-phytofluene + reduced acceptor

16 entries

15-cis-phytoene + acceptor

all-trans-zeta-carotene + reduced acceptor

12 entries

15-cis-phytoene + FAD

all-trans-phytofluene + FADH2

2 entries

all-trans-neurosporene + acceptor

lycopene + reduced acceptor

2 entries

all-trans-neurosporene + FAD

lycopene + FADH2

2 entries

all-trans-phytofluene + 2 acceptor

all-trans-neurosporene + 2 reduced acceptor

Rhodobacter capsulatus

-

-

712316

-

-

?

all-trans-phytofluene + acceptor

all-trans-zeta-carotene + reduced acceptor

16 entries

all-trans-phytofluene + FAD

all-trans-zeta-carotene + FADH2

Rubrivivax gelatinosus

-

-

711020

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

27 entries

all-trans-zeta-carotene + FAD

all-trans-neurosporene + FADH2

Rubrivivax gelatinosus

-

-

711020

-

-

?

phytoene + acceptor

neurosporene + reduced acceptor

Cereibacter sphaeroides

-

-

712351

-

-

?

additional information

?

-

5 entries

15-cis-phytoene + 3 acceptor

all-trans-neurosporene + 3 reduced acceptor

Cereibacter sphaeroides

-

the enzyme is involved in carotenoid biosynthesis

712351

-

-

?

15-cis-phytoene + 3 acceptor

all-trans-neurosporene + 3 reduced acceptor

Rhodobacter capsulatus

-

-

713598

formation of all-trans-neurosporene and two cis-isomers

-

?

15-cis-phytoene + 3 acceptor

all-trans-neurosporene + 3 reduced acceptor

Rhodobacter capsulatus

-

involved in carotenoid biosynthesis

712316

-

-

?

15-cis-phytoene + 3 acceptor

all-trans-neurosporene + 3 reduced acceptor

Rhodobacter capsulatus

-

intermediates are phytofluene and zeta-carotene. The proportion of all-trans versus cis isomers is about 20% for phytofluene, 46% for zeta-carotene, and 72% for neurosporene

712316

-

-

?

15-cis-phytoene + acceptor

all-trans-phytofluene + reduced acceptor

Cereibacter azotoformans

G3FHJ1

-

725042

-

-

?

15-cis-phytoene + acceptor

all-trans-phytofluene + reduced acceptor

Cereibacter azotoformans CGMCC 6086

G3FHJ1

-

725042

-

-

?

15-cis-phytoene + acceptor

all-trans-phytofluene + reduced acceptor

Cereibacter sphaeroides

P54980

-

759253

-

-

?

15-cis-phytoene + acceptor

all-trans-phytofluene + reduced acceptor

Cereibacter sphaeroides ATCC 17023

P54980

-

759253

-

-

?

15-cis-phytoene + acceptor

all-trans-phytofluene + reduced acceptor

Cereibacter sphaeroides ATH 2.4.1.

P54980

-

759253

-

-

?

15-cis-phytoene + acceptor

all-trans-phytofluene + reduced acceptor

Cereibacter sphaeroides CCUG 31486

P54980

-

759253

-

-

?

15-cis-phytoene + acceptor

all-trans-phytofluene + reduced acceptor

Cereibacter sphaeroides DSM 158

P54980

-

759253

-

-

?

15-cis-phytoene + acceptor

all-trans-phytofluene + reduced acceptor

Cereibacter sphaeroides JCM 6121

P54980

-

759253

-

-

?

15-cis-phytoene + acceptor

all-trans-phytofluene + reduced acceptor

Cereibacter sphaeroides LMG 2827

P54980

-

759253

-

-

?

15-cis-phytoene + acceptor

all-trans-phytofluene + reduced acceptor

Cereibacter sphaeroides NBRC 12203

P54980

-

759253

-

-

?

15-cis-phytoene + acceptor

all-trans-phytofluene + reduced acceptor

Cereibacter sphaeroides NCIMB 8253

P54980

-

759253

-

-

?

15-cis-phytoene + acceptor

all-trans-phytofluene + reduced acceptor

Rhodobacter capsulatus

-

-

713598

-

-

?

15-cis-phytoene + acceptor

all-trans-phytofluene + reduced acceptor

Rhodobacter capsulatus

P17054

-

759253

-

-

?

15-cis-phytoene + acceptor

all-trans-phytofluene + reduced acceptor

Rhodobacter capsulatus ATCC BAA-309

P17054

-

759253

-

-

?

15-cis-phytoene + acceptor

all-trans-phytofluene + reduced acceptor

Rhodobacter capsulatus NBRC 16581

P17054

-

759253

-

-

?

15-cis-phytoene + acceptor

all-trans-phytofluene + reduced acceptor

Rhodobacter capsulatus SB1003

P17054

-

759253

-

-

?

15-cis-phytoene + acceptor

all-trans-zeta-carotene + reduced acceptor

Brevibacterium linens

-

-

724038

-

-

?

15-cis-phytoene + acceptor

all-trans-zeta-carotene + reduced acceptor

Brevibacterium linens DSM 20426

-

-

724038

-

-

?

15-cis-phytoene + acceptor

all-trans-zeta-carotene + reduced acceptor

Cereibacter sphaeroides

-

-

724038

-

-

?

15-cis-phytoene + acceptor

all-trans-zeta-carotene + reduced acceptor

Cereibacter sphaeroides KCTC 12085

-

-

724038

-

-

?

15-cis-phytoene + acceptor

all-trans-zeta-carotene + reduced acceptor

Corynebacterium glutamicum

-

-

724038

-

-

?

15-cis-phytoene + acceptor

all-trans-zeta-carotene + reduced acceptor

Corynebacterium glutamicum KCTC 1445

-

-

724038

-

-

?

15-cis-phytoene + acceptor

all-trans-zeta-carotene + reduced acceptor

Pantoea agglomerans

K7WPN7

-

724038

-

-

?

15-cis-phytoene + acceptor

all-trans-zeta-carotene + reduced acceptor

Pantoea agglomerans KCTC 2479

K7WPN7

-

724038

-

-

?

15-cis-phytoene + acceptor

all-trans-zeta-carotene + reduced acceptor

Rhodobacter capsulatus

-

-

724038

-

-

?

15-cis-phytoene + acceptor

all-trans-zeta-carotene + reduced acceptor

Rhodobacter capsulatus KCTC 2583

-

-

724038

-

-

?

15-cis-phytoene + acceptor

all-trans-zeta-carotene + reduced acceptor

Rhodopirellula baltica

-

-

724038

-

-

?

15-cis-phytoene + acceptor

all-trans-zeta-carotene + reduced acceptor

Rhodopirellula baltica DSM 10527

-

-

724038

-

-

?

15-cis-phytoene + FAD

all-trans-phytofluene + FADH2

Rubrivivax gelatinosus

-

-

711020

-

-

?

15-cis-phytoene + FAD

all-trans-phytofluene + FADH2

Rubrivivax gelatinosus CGMCC 6086

-

-

711020

-

-

?

all-trans-neurosporene + acceptor

lycopene + reduced acceptor

Cereibacter azotoformans

G3FHJ1

-

725042

-

-

?

all-trans-neurosporene + acceptor

lycopene + reduced acceptor

Cereibacter azotoformans CGMCC 6086

G3FHJ1

-

725042

-

-

?

all-trans-neurosporene + FAD

lycopene + FADH2

Rubrivivax gelatinosus

-

-

711020

-

-

?

all-trans-neurosporene + FAD

lycopene + FADH2

Rubrivivax gelatinosus CGMCC 6086

-

-

711020

-

-

?

all-trans-phytofluene + acceptor

all-trans-zeta-carotene + reduced acceptor

Cereibacter azotoformans

G3FHJ1

-

725042

-

-

?

all-trans-phytofluene + acceptor

all-trans-zeta-carotene + reduced acceptor

Cereibacter azotoformans CGMCC 6086

G3FHJ1

-

725042

-

-

?

all-trans-phytofluene + acceptor

all-trans-zeta-carotene + reduced acceptor

Cereibacter sphaeroides

P54980

-

759253

-

-

?

all-trans-phytofluene + acceptor

all-trans-zeta-carotene + reduced acceptor

Cereibacter sphaeroides ATCC 17023

P54980

-

759253

-

-

?

all-trans-phytofluene + acceptor

all-trans-zeta-carotene + reduced acceptor

Cereibacter sphaeroides ATH 2.4.1.

P54980

-

759253

-

-

?

all-trans-phytofluene + acceptor

all-trans-zeta-carotene + reduced acceptor

Cereibacter sphaeroides CCUG 31486

P54980

-

759253

-

-

?

all-trans-phytofluene + acceptor

all-trans-zeta-carotene + reduced acceptor

Cereibacter sphaeroides DSM 158

P54980

-

759253

-

-

?

all-trans-phytofluene + acceptor

all-trans-zeta-carotene + reduced acceptor

Cereibacter sphaeroides JCM 6121

P54980

-

759253

-

-

?

all-trans-phytofluene + acceptor

all-trans-zeta-carotene + reduced acceptor

Cereibacter sphaeroides LMG 2827

P54980

-

759253

-

-

?

all-trans-phytofluene + acceptor

all-trans-zeta-carotene + reduced acceptor

Cereibacter sphaeroides NBRC 12203

P54980

-

759253

-

-

?

all-trans-phytofluene + acceptor

all-trans-zeta-carotene + reduced acceptor

Cereibacter sphaeroides NCIMB 8253

P54980

-

759253

-

-

?

all-trans-phytofluene + acceptor

all-trans-zeta-carotene + reduced acceptor

Rhodobacter capsulatus

-

-

713598

-

-

?

all-trans-phytofluene + acceptor

all-trans-zeta-carotene + reduced acceptor

Rhodobacter capsulatus

P17054

-

759253

-

-

?

all-trans-phytofluene + acceptor

all-trans-zeta-carotene + reduced acceptor

Rhodobacter capsulatus ATCC BAA-309

P17054

-

759253

-

-

?

all-trans-phytofluene + acceptor

all-trans-zeta-carotene + reduced acceptor

Rhodobacter capsulatus NBRC 16581

P17054

-

759253

-

-

?

all-trans-phytofluene + acceptor

all-trans-zeta-carotene + reduced acceptor

Rhodobacter capsulatus SB1003

P17054

-

759253

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Brevibacterium linens

-

-

724038

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Brevibacterium linens DSM 20426

-

-

724038

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Cereibacter azotoformans

G3FHJ1

-

725042

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Cereibacter azotoformans CGMCC 6086

G3FHJ1

-

725042

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Cereibacter sphaeroides

-

-

724038

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Cereibacter sphaeroides

P54980

-

759253

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Cereibacter sphaeroides ATCC 17023

P54980

-

759253

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Cereibacter sphaeroides ATH 2.4.1.

P54980

-

759253

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Cereibacter sphaeroides CCUG 31486

P54980

-

759253

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Cereibacter sphaeroides DSM 158

P54980

-

759253

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Cereibacter sphaeroides JCM 6121

P54980

-

759253

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Cereibacter sphaeroides KCTC 12085

-

-

724038

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Cereibacter sphaeroides LMG 2827

P54980

-

759253

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Cereibacter sphaeroides NBRC 12203

P54980

-

759253

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Cereibacter sphaeroides NCIMB 8253

P54980

-

759253

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Corynebacterium glutamicum

-

-

724038

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Corynebacterium glutamicum KCTC 1445

-

-

724038

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Pantoea agglomerans

K7WPN7

-

724038

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Pantoea agglomerans KCTC 2479

K7WPN7

-

724038

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Rhodobacter capsulatus

-

-

712316, 713598, 724038

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Rhodobacter capsulatus

P17054

-

759253

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Rhodobacter capsulatus ATCC BAA-309

P17054

-

759253

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Rhodobacter capsulatus KCTC 2583

-

-

724038

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Rhodobacter capsulatus NBRC 16581

P17054

-

759253

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Rhodobacter capsulatus SB1003

P17054

-

759253

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Rhodopirellula baltica

-

-

724038

-

-

?

all-trans-zeta-carotene + acceptor

all-trans-neurosporene + reduced acceptor

Rhodopirellula baltica DSM 10527

-

-

724038

-

-

?

additional information

?

-

Cereibacter azotoformans

G3FHJ1

at higher concentrations, phytoene is the preferred substrate for CrtI, and neurosporene is produced as the major desaturation product. At lower phytoene concentrations, neurosporene can be further desaturated by CrtI to produce lycopene, EC 1.3.99.31

725042

-

-

?

additional information

?

-

Cereibacter azotoformans CGMCC 6086

G3FHJ1

at higher concentrations, phytoene is the preferred substrate for CrtI, and neurosporene is produced as the major desaturation product. At lower phytoene concentrations, neurosporene can be further desaturated by CrtI to produce lycopene, EC 1.3.99.31

725042

-

-

?

additional information

?

-

Rhodobacter capsulatus

-

C30-diapophytoene is not a substrate

712316

-

-

?

additional information

?

-

Rubrivivax gelatinosus

-

the phytoene desaturase CrtI from Rubrivivax gelatinosus catalyzes simultaneously a three- and four-step desaturation producing both neurosporene and lycopene, determination of kinetic with the L208 modified desaturase and the specificities for phytoene and neurosporene as substrates, overview

711020

-

-

?

additional information

?

-

Rubrivivax gelatinosus CGMCC 6086

-

the phytoene desaturase CrtI from Rubrivivax gelatinosus catalyzes simultaneously a three- and four-step desaturation producing both neurosporene and lycopene, determination of kinetic with the L208 modified desaturase and the specificities for phytoene and neurosporene as substrates, overview

711020

-

-

?