1.3.99.28: phytoene desaturase (neurosporene-forming)
This is an abbreviated version!
For detailed information about phytoene desaturase (neurosporene-forming), go to the full flat file.
Reaction
+ 3 acceptor = + 3 reduced acceptor
Synonyms
3-step phytoene desaturase, CrtI, PDS, phytoene desaturase
ECTree
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Engineering
Engineering on EC 1.3.99.28 - phytoene desaturase (neurosporene-forming)
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F166I
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mutation changes the product of phytoene desaturation from neurosporene to lycopene
M402T
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mutation has a negative effect on percent lycopene production
V68D
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mutation changes the product of phytoene desaturation from neurosporene to lycopene
L153P/L278P
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site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme
L208F
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site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme
L208P
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site-directed mutagenesis, the mutant shows altered secondary structure compared to the wild-type enzyme
T256M/D355G/L424P
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site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme
Y44C/D53G/P134L/V395A
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site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme
additional information
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functional complementation of heterogeneous phytoene desaturases (CrtIs) from 5 carotenogenic microorganisms, overview
additional information
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functional complementation of heterogeneous phytoene desaturases (CrtIs) from 5 carotenogenic microorganisms, overview
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additional information
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directed evolution is used to change the product of Rhodobacter sphaeroides phytoene desaturase (crtI gene product), a neurosporene-producing enzyme, to lycopene. Two generations of random mutagenesis are performed, from which three positive mutants are isolated and sequenced. Site-directed mutagenesis is used to determine the effect of each amino acid change
additional information
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functional complementation of heterogeneous phytoene desaturases (CrtIs) from 5 carotenogenic microorganisms, overview
additional information
competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway
additional information
Cereibacter sphaeroides ATCC 17023
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competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway
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additional information
Cereibacter sphaeroides ATH 2.4.1.
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competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway
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additional information
Cereibacter sphaeroides CCUG 31486
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competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway
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additional information
Cereibacter sphaeroides DSM 158
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competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway
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additional information
Cereibacter sphaeroides JCM 6121
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competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway
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additional information
Cereibacter sphaeroides KCTC 12085
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functional complementation of heterogeneous phytoene desaturases (CrtIs) from 5 carotenogenic microorganisms, overview
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additional information
Cereibacter sphaeroides LMG 2827
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competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway
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additional information
Cereibacter sphaeroides NBRC 12203
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competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway
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additional information
Cereibacter sphaeroides NCIMB 8253
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competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway
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additional information
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functional complementation of heterogeneous phytoene desaturases (CrtIs) from 5 carotenogenic microorganisms, overview
additional information
-
functional complementation of heterogeneous phytoene desaturases (CrtIs) from 5 carotenogenic microorganisms, overview
-
additional information
functional complementation of heterogeneous phytoene desaturases (CrtIs) from 5 carotenogenic microorganisms, overview
additional information
-
functional complementation of heterogeneous phytoene desaturases (CrtIs) from 5 carotenogenic microorganisms, overview
-
additional information
-
functional complementation of heterogeneous phytoene desaturases (CrtIs) from 5 carotenogenic microorganisms, overview
additional information
competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway
additional information
-
competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway
-
additional information
-
functional complementation of heterogeneous phytoene desaturases (CrtIs) from 5 carotenogenic microorganisms, overview
-
additional information
-
competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway
-
additional information
-
competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway
-
additional information
-
functional complementation of heterogeneous phytoene desaturases (CrtIs) from 5 carotenogenic microorganisms, overview
additional information
-
functional complementation of heterogeneous phytoene desaturases (CrtIs) from 5 carotenogenic microorganisms, overview
-