Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.3.99.28: phytoene desaturase (neurosporene-forming)

This is an abbreviated version!
For detailed information about phytoene desaturase (neurosporene-forming), go to the full flat file.

Reaction

15-cis-phytoene
+ 3 acceptor =
all-trans-neurosporene
+ 3 reduced acceptor

Synonyms

3-step phytoene desaturase, CrtI, PDS, phytoene desaturase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.99 With unknown physiological acceptors
                1.3.99.28 phytoene desaturase (neurosporene-forming)

Engineering

Engineering on EC 1.3.99.28 - phytoene desaturase (neurosporene-forming)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F166I
-
mutation changes the product of phytoene desaturation from neurosporene to lycopene
H12Q
-
mutation has little effect on the product formation
M402T
-
mutation has a negative effect on percent lycopene production
V68D
-
mutation changes the product of phytoene desaturation from neurosporene to lycopene
L153P/L278P
-
site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme
L208F
-
site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme
L208P
-
site-directed mutagenesis, the mutant shows altered secondary structure compared to the wild-type enzyme
T256M/D355G/L424P
-
site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme
Y44C/D53G/P134L/V395A
-
site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme
additional information