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1.3.98.1: dihydroorotate dehydrogenase (fumarate)

This is an abbreviated version!
For detailed information about dihydroorotate dehydrogenase (fumarate), go to the full flat file.

Word Map on EC 1.3.98.1

Reaction

(S)-dihydroorotate
+
fumarate
=
Orotate
 +
succinate

Synonyms

(DHO) dehydrogenase, 4,5-L-dihydroorotate:oxygen oxidoreductase, ACT/DHOD, class 1A DHOD, class 1A DHODH, class 1A dihydroorotate dehydrogenase, DHOD, DHOD1, DHOD2, DHOD3, DHODA, DHOdehase, DHODH, DHODH-1A, dihydroorotate dehydrogenase, dihydroorotate dehydrogenase class 1A, Dihydroorotate oxidase, EC 1.3.3.1, LmDHODH, More, oxidase, dihydroorotate, TcDHOD

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.98 With other, known, physiological acceptors
                1.3.98.1 dihydroorotate dehydrogenase (fumarate)

Purification

Purification on EC 1.3.98.1 - dihydroorotate dehydrogenase (fumarate)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
acid treatment, DE-52-, Matrex Orange A-, Matrex Blue A column chromatography
-
ammonium sulfate precipitation and Q-Sepharose column chromatography
-
by ion-exchange chromatography
-
centrifugation after cell disruption, separation from the NADP-linked dihydroorotic dehydrogenase
-
ion-exchange, gel filtration, ammonium sulfate precipitation
-
native enzyme from the cytosolic fraction by gel filtration and immunoaffinity chromatography, recombinant His-tagged enzyme by nickel affinity chromatography to homogeneity and subsequent clevage of the tag by thrombin
-
Ni2+-NTA agarose chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration
-
Orange-A column, reverse-affinity step, Mono Q column
-
preparative gel electrophoresis
-
recombinant DHOD1, DHOD2 and DHOD3 with an N-terminal His6-tag, by affinity chromatography
recombinant His-tagged enzyme by nickel affinity chromatography
-
soniciation and gel filtration
streptomycin precipitation, ion-exchange, hydroxylapatite
-
streptomycin sulfate and ammonium sulfate precipitation, DEAE-Sephacel-, affinity chromatography
-
the enzyme is fused with aspartate carbamoyltransferase as precursor to mature DHOD
-
to homogeneity by a single affinity chromatography step
-
to homogeneity by a single nickel affinity chromatography step
-
to homogeneity by ion-exchange, hydrophobic interaction and gel filtration