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1.3.8.6: glutaryl-CoA dehydrogenase (ETF)

This is an abbreviated version!
For detailed information about glutaryl-CoA dehydrogenase (ETF), go to the full flat file.

Word Map on EC 1.3.8.6

Reaction

(E)-glutaconyl-CoA
=
crotonyl-CoA
 +
CO2

Synonyms

decarboxylating glutaryl-coenzyme A dehydrogenase, EC 1.3.99.7, GCD, GCDH, GDH, GDHGeo, glutaryl coenzyme A dehydrogenase, glutaryl-CoA dehydrogenase, glutaryl-coenzyme A dehydrogenase, More

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.8 With a flavin as acceptor
                1.3.8.6 glutaryl-CoA dehydrogenase (ETF)

Crystallization

Crystallization on EC 1.3.8.6 - glutaryl-CoA dehydrogenase (ETF)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified detagged recombinant apoenzyme without FAD, sitting drop vapor diffusion method, 26 mg/ml protein in 25 mM Tris pH 8.0, 200 mM NaCl, 1% glycerol, and 1 mM TCEP, with reservoir solution consisting of 20% PEG 3000, 100 mM HEPES, 200 mM NaCl, pH 7.5, 16°C, X-ray diffraction structure determination and analysis at 1.99-2.40 A resolution
15 mg/ml purified recombinant enzyme in 20 mM HEPES, pH 7.5, 100 mM NaCl, enzyme is free or in complex with 4-nitrobutyryl-CoA, hanging drop vapour diffusion method, equal volumes of protein and precipitant solution, the latter containing 100 mM MES, pH 6.5, 30% PEG monomethyl ether 5000, 0.2 M ammonium sulfate, 19°C, addition of 0.1% octyl beta-D-glucopyranoside, 0.4 mM acetoacetyl-CoA, with or without addition of 0.4 mM 4-nitrobutyryl-CoA, X-ray diffraction structure determination and analysis at 2.1 A and 2.6 A resolution, respectively
hanging drop vapour diffusion method with 100 mM MES buffer, pH 6.5, 30% poly(ethyleneglycol) monomethyl ether 5000, and 0.2 M ammonium sulfate, at 19°C, in complex with 4-nitrobutyric acid
purified GDH, hanging drop vapor diffusion method, 10 mg/ml protein in 10 mM MES, pH 6.0, 0.5 M KCl, 10% w/v glycerol, 1 mM DTT, 1 mM FAD, and glutaryl-CoA, are mixed with an equal volume of reservoir solution containing 15% v/v MPD, 0.1 M imidazole, pH 6.5, 0.2 M KCl, and 2% PEG 3350, at 4°C, 2 days, X-ray diffraction structure determination and analysis