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1.3.8.4: isovaleryl-CoA dehydrogenase

This is an abbreviated version!
For detailed information about isovaleryl-CoA dehydrogenase, go to the full flat file.

Word Map on EC 1.3.8.4

Reaction

3-methylbutanoyl-CoA
+
electron-transfer flavoprotein
=
3-methylbut-2-enoyl-CoA
 +
reduced electron-transfer flavoprotein

Synonyms

acyl-CoA dehydrogenase, EC 1.3.99.10, i3VD, iso(3)valeryl-CoA dehydrogenase, isovaleric-CoA dehydrogenase, isovaleroyl-coenzyme A dehydrogenase, isovaleryl-coenzyme A dehydrogenase, IVD, IVDH, LiuA, Pden_3633, SBCAD, short/branched chain acyl-CoA dehydrogenase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.8 With a flavin as acceptor
                1.3.8.4 isovaleryl-CoA dehydrogenase

Specific Activity

Specific Activity on EC 1.3.8.4 - isovaleryl-CoA dehydrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00003
-
intact mitochondria, no added acceptor
0.000039
-
mitochondria from isovaleric acidemia cells, 13% relative activity to normal cells, specific activity determined by tritium release assay
0.00016
-
sonicated mitochondria, no added acceptor
0.000163
-
mitochondria from isovaleric acidemia cells, 12% relative activity to normal cells, specific activity determined by dye reduction assay
0.0003
-
recombinant mutant L95V/A99V/L103V/L370M/G374A, substrate 2-methylbutanoyl-CoA
0.00031
-
mitochondria from normal cells, specific activity determined by tritium release assay
0.00034
fibroblast cell line harboring the mutation corresponding to the A282V mutant
0.00044
-
sonicated mitochondria, acceptor added: electron-transferring flavoprotein
0.00046
-
sonicated mitochondria, acceptor added: phenazine methosulfate
0.0011
-
recombinant mutant L370M/G374A, substrate 2-methylbutanoyl-CoA
0.00131
-
mitochondria from normal cells, specific activity determined by dye reduction assay
0.004
cell free extract of Escherichia coli cells expressing the enzyme mutant R363C
0.0041
0.0043
-
recombinant wild-type enzyme, substrate 2-methylbutanoyl-CoA
0.005
-
sonicate
0.0062
-
purified enzyme in the absence of electron transfer agents, reaction terminated with HCl or with KMnO4/HCl
0.01
-
propionyl-CoA as substrate and phenazine methosulfate as acceptor or hexanoyl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor
0.0114
-
recombinant mutant L370M/G374A, substrate butyryl-CoA
0.012
-
Triton X-100 extract
0.0125
-
sonicate supernatant fraction
0.0136
-
purified enzyme with phenazine methosulfate and dichlorophenol indophenol, reaction terminated with HCl
0.014
-
soluble extract
0.0152
-
recombinant wild-type enzyme, substrate butyryl-CoA
0.0207
-
purified enzyme with electron-transferring flavoprotein and 0.062 mM dichlorophenol indophenol, reaction terminated with HCl, concentration of isovaleryl-CoA: 0.05 mM, specific activity determined by tritium release assay
0.0239
-
recombinant mutant L370M/G374A, substrate valeryl-CoA
0.032
cell free extract of Escherichia coli cells expressing the enzyme mutant R382L
0.0337
-
purified enzyme, acceptor added: electron-transferring flavoprotein
0.0364
-
recombinant wild-type enzyme, substrate valeryl-CoA
0.0371
-
recombinant mutant L370M/G374A, substrate isovaleryl-CoA
0.043
0.0472
-
purified enzyme with phenazine methosulfate and dichlorophenol indophenol, reaction terminated with KMnO4/HCl
0.052
cell free extract of Escherichia coli cells expressing the enzyme mutant V342A
0.0572
-
purified enzyme with electron-transferring flavoprotein and 0.062 mM dichlorophenol indophenol, reaction terminated with KMnO4/HCl, concentration of isovaleryl-CoA: 0.05 mM, specific activity determined by tritium release assay
0.0754
-
recombinant wild-type enzyme, substrate isovaleryl-CoA
0.08
-
substrate: octanoyl-CoA
0.086
0.09
-
valeryl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor
0.17
-
S-2-methylbutyryl-CoA as substrate and phenazine methosulfate as acceptor
0.172
-
purified enzyme, acceptor added: electron-transferring flavoprotein and 0.062 mM dichlorophenol indophenol, concentration of isovaleryl-CoA: 0.02 mM, specific activity determined by dye reduction assay
0.3
-
partially purified enzyme, substrate: isovaleryl-CoA
0.44
cell free extract of Escherichia coli cells expressing the enzyme, wild type
0.51
-
butyryl-CoA as substrate and phenazine methosulfate as acceptor
0.53
-
isovaleryl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor
0.6
of purified recombinant V342A mutant, electron acceptor: electron-transferring flavoprotein
0.64
-
hexanoyl-CoA as substrate and phenazine methosulfate as acceptor
0.72
-
isovaleryl-CoA as substrate and 0.012 mM electron transfer flavoprotein as acceptor
0.832
1
-
substrate: isovaleryl-CoA
1.09
-
valeryl-CoA as substrate and phenazine methosulfate as acceptor
1.19
-
substrate: hexanoyl-CoA
1.24
of purified recombinant V342A mutant, electron acceptor: dichlorophenol indophenol + FAD
1.27
of purified recombinant V342A mutant, electron acceptor: dichlorophenol indophenol
1.4
of purified recombinant R382L mutant, electron acceptor: electron-transferring flavoprotein
1.71
-
substrate: butyryl-CoA
10.3
of purified recombinant wild-type enzyme, electron acceptor: dichlorophenol indophenol + FAD
11.7
of purified recombinant wild-type enzyme, electron acceptor: electron-transferring flavoprotein
2.04
of purified recombinant A282V mutant, electron acceptor: dichlorophenol indophenol
2.2
of purified recombinant A282V mutant, electron acceptor: electron-transferring flavoprotein
2.33
of purified recombinant A282V mutant, electron acceptor: dichlorophenol indophenol + FAD
2.91
-
isovaleryl-CoA as substrate and phenazine methosulfate as acceptor
3.71
-
substrate: valeryl-CoA
4.88
of purified recombinant R382L mutant, electron acceptor: dichlorophenol indophenol
6.34
of purified recombinant R382L mutant, electron acceptor: dichlorophenol indophenol + FAD
8.1
-
substrate: isovaleryl-CoA
8.2
of purified recombinant wild-type enzyme, electron acceptor: dichlorophenol indophenol
additional information
-
enzyme assay development and optimization