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1.3.7.2: 15,16-dihydrobiliverdin:ferredoxin oxidoreductase

This is an abbreviated version!
For detailed information about 15,16-dihydrobiliverdin:ferredoxin oxidoreductase, go to the full flat file.

Word Map on EC 1.3.7.2

Reaction

15,16-dihydrobiliverdin
+
oxidized ferredoxin
=
biliverdin IXalpha
+
reduced ferredoxin
+ 2 H+

Synonyms

15,16-DHBV:ferredoxin oxidoreductase, 15,16-dihydrobiliverdin:ferredoxin oxidoreductase, BRADO1265, DHBV:ferredoxin oxidoreductase, FDBR, ferredoxin-dependent bilin reductase, ferredoxin:15,16-dihydrobiliverdin oxidoreductase, GtPEBA, oxidoreductase, ferredoxin:15,16-dihydrobiliverdin, PcyA, PebA

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.7 With an iron-sulfur protein as acceptor
                1.3.7.2 15,16-dihydrobiliverdin:ferredoxin oxidoreductase

Purification

Purification on EC 1.3.7.2 - 15,16-dihydrobiliverdin:ferredoxin oxidoreductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by two affinity chromatography steps to 90% purity and a third gel filtration step
GST-tagged PebA forms inactive aggregates
recombinant GST-tagged enzyme from Escherichia coli by ultrafiltration, glutathione affinity chromatography, and gel filtration
recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, ultrafiltration, and gel filtration
-
recombinant GST-tagged PebA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, gel filtration, and ultrafiltration, recombinant tagged PebAgB by affinity chromatography