Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.3.7.15: chlorophyllide a reductase

This is an abbreviated version!
For detailed information about chlorophyllide a reductase, go to the full flat file.

Word Map on EC 1.3.7.15

Reaction

3-deacetyl-3-(1-hydroxyethyl)bacteriochlorophyllide a
+ 2 oxidized ferredoxin [iron-sulfur] cluster +
ADP
 +
phosphate
=
3-devinyl-3-(1-hydroxyethyl)chlorophyllide a
 + 2 reduced ferredoxin [iron-sulfur] cluster +
ATP
 +
H2O
 + 2 H+

Synonyms

(BchY/BchZ)2 protein, a-COR, BchA, bchB, bchL, bchN, BchX, BchX/Y/Z, BchXYZ, BchY, BchZ, chlorophyllide a oxidoreductase, chlorophyllide oxidoreductase, chlorophyllide reductase, COR, COR 8VR, dark-operative protochlorophyllide oxidoreductase, DPOR, EC 1.3.99.35, light-independent chlorophyllide reductase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.7 With an iron-sulfur protein as acceptor
                1.3.7.15 chlorophyllide a reductase

Cloned

Cloned on EC 1.3.7.15 - chlorophyllide a reductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
genes bchX and bchY, recombinant expression of GST-tagged BchX and BchY in Escherichia coli
genes bchX, bchY, and bchZ, detailed phylogenetic analysis and tree of BchXYZ
recombinant overexpression of enzymatically active subunit (BchY/BchZ)2 and BchX2 subcomplexes in Escherichia coli strain BL21(DE3), expression of N-terminally His6-tagged BchY, N-terminally thioredoxin/His/S-tagged BchX2, and untagged BchZ under the control of individual promoters. Supplementation with Fe(III)citrate and L-cysteine as sources of iron and sulfur, respectively, as media constituents has significant influence on the in vivo assembly of [4Fe-4S] clusters, growth temperature is 17°C
P26277; Q16DU8; Q16DU7
reconstitution of chlorophyllide a reductase with purified proteins. Two Rhodobacter capsulatus strains that overexpress Strep-tagged BchX and BchY are isolated. Strep-tagged BchX is purified as a single polypeptide, and BchZ is co-purified with Strep-tagged BchY. When BchX and BchY-BchZ components are incubated with chlorophyllide a, ATP, and dithionite under anaerobic conditions, chlorophyllide a is converted to a new pigment
-