1.3.5.5: 15-cis-phytoene desaturase
This is an abbreviated version!
For detailed information about 15-cis-phytoene desaturase, go to the full flat file.
Word Map on EC 1.3.5.5
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1.3.5.5
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carotenoid
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herbicide
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zeta-carotene
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agriculture
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bleaching
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norflurazon
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synthesis
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phytofluene
- 1.3.5.5
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carotenoid
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herbicide
- zeta-carotene
- agriculture
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bleaching
- norflurazon
- synthesis
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phytofluene
Reaction
Synonyms
BoPDS, CitPDS1, CrtP, OsPDS, PDS, PDS1, PDS3, phytoene desaturase, plant-type phytoene desaturase
ECTree
1.3.5.5 15-cis-phytoene desaturaseAdvanced search results
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Cofactor on EC 1.3.5.5 - 15-cis-phytoene desaturase
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COFACTOR 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
decyl-plastoquinone
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this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially
quinone
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since phytoene desaturation responds to the redox state of quinones, which is adjusted by the respiratory redox pathway, the two reactions must be regarded as being mechanistically linked. Using redox titrations, it is shown that the optimal activity lies in the range of the midpoint potential of the plastoquinone/plastohydroquinone redox couple
Ubiquinone-10
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this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially
FAD
the deduced amino acid sequence of wheat phytoene desaturase reveals a typical pyridine dinucleotide binding motif generally associated with the binding of FAD
FAD
PDS requires plastoquinone as a directly interacting co-substrate to reoxidize the enzyme-bound FADred formed upon desaturation
plastoquinone
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decyl-plastoquinone or decyl-ubiquinone is used in the study because the natural C45-plastoquinone is not available commercially. The affinity of the enzyme for plastoquinone is higher than for NADP+
plastoquinone
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plastoquinone is an essential component of phytoene desaturation
additional information
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5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole, 2,5-dibromo-3-methyl-6-isopropyl-l,4-benzoquinone, duroquinon are used as artificial electron acceptor
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additional information
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the enzyme also shows activity with NADP+ as cosubstrate, the affinity for NADP+ is lower than for decylplastoquinone or decylubioquinone
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additional information
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the soluble form of the enzyme is able to associate on to/into protein-free liposomal membranes made from chromoplast lipids, thereby gaining activity by binding added FAD. Once bound to membranes, activated phytoene desaturase works independently of any added FAD, employing membrane-bound electron acceptors. FAD exerts no positive effect on the membrane-association process, its role is confined to enzymatic activation
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