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1.3.5.3: protoporphyrinogen IX dehydrogenase (quinone)

This is an abbreviated version!
For detailed information about protoporphyrinogen IX dehydrogenase (quinone), go to the full flat file.

Reaction

protoporphyrinogen IX
+ 3 quinone =
protoporphyrin IX
+ 3 quinol

Synonyms

HemG, HemG protein, PPO, PPOX, PPX, PPX1, protogen oxidase, protoporphyrinogen IX dehydrogenase (menaquinone), protoporphyrinogen IX oxidase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.5 With a quinone or related compound as acceptor
                1.3.5.3 protoporphyrinogen IX dehydrogenase (quinone)

Purification

Purification on EC 1.3.5.3 - protoporphyrinogen IX dehydrogenase (quinone)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme solubilized from membranes by anion exchange chromatography, recombinant His-tagged enzyme from Bacillus megaterium byy affinity chromatography and gel filtration. Removal of the fused His-tag via protease digestion does not change the catalytic properties
-
six histidine-tagged recombinant protein
-