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1.3.3.8: tetrahydroberberine oxidase

This is an abbreviated version!
For detailed information about tetrahydroberberine oxidase, go to the full flat file.

Word Map on EC 1.3.3.8

Reaction

(S)-tetrahydroberberine
+ 2 O2 +
H+
=
Berberine
+ 2 H2O2

Synonyms

(S)-H4Ber oxidase, (S)-tetrahydroberberine oxidase, (S)-THB oxidase, EC 1.5.3.8, oxidase, (S)-tetrahydroberberine, THBO

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.3 With oxygen as acceptor
                1.3.3.8 tetrahydroberberine oxidase

Cloned

Cloned on EC 1.3.3.8 - tetrahydroberberine oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA is expressed in Escherichia coli DH5alpha
-
DNA and amino acid sequence determination and analysis, functional ectopical expression of CjTHBO under the control of Cauliflower mosaic virus 35S promoterin Eschscholzia californica cells via transformation with Agrobacterium tumefaciens strain LBA4404. The transgenic cells produce coptisine and dehydrocheilanthifoline, which are expected to be generated by the reaction of CjTHBO from pathway intermediates, i.e. cheilanthifoline and stylopine, alkaloid profile analysis in transgenic California poppy cells, overview. Expression in Escherichia coli strain BL21 (DE3)
gene Cs(S)-THBO, DNA and amino acid sequence determination and analysis, real-time quantitative PCR analysis, tissue expression profiling analyses, expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3)