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1.3.1.9: enoyl-[acyl-carrier-protein] reductase (NADH)

This is an abbreviated version!
For detailed information about enoyl-[acyl-carrier-protein] reductase (NADH), go to the full flat file.

Word Map on EC 1.3.1.9

Reaction

an acyl-[acyl-carrier protein]
+
NAD+
=
a trans-2,3-dehydroacyl-[acyl-carrier protein]
+
NADH
+
H+

Synonyms

2-trans enoyl-ACP-reductase, 2-trans enoyl-acyl carrier protein reductase, 2-trans-enoyl-ACP (CoA) reductase, 2-trans-enoyl-ACP reductase, 2-trans-enoyl-ACP(CoA) reductase, ACP reductase, BaENR, BMA0885, bsFabI, cold-shock induced protein 15, CSI15, EACP reductase, enoyl (acyl carrier protein) reductase, enoyl ACP reductase, enoyl acyl carrier protein reductase, enoyl acyl carrier protein reductase InhA, enoyl reductase, enoyl-ACP reductase, enoyl-ACP reductase I, enoyl-ACP reductase III, enoyl-ACP(CoA) reductase, enoyl-acyl carrier protein, enoyl-acyl carrier protein reductase, enoyl-acyl carrier protein reductase I, enoyl-reductase, enoyl-[acyl-carrier-protein] reductase, ENR, ENR1, ENR2, FabI, FabI-1, FabI-related enoyl-ACP reductase, FabI1, FabI2, FabK, Fabl1, Fabl2, FabMG, FabV, FAS-II enoyl reductase, FTT_0782, InhA, More, MtENR, MtInhA, NAD-dependent enoyl-ACP reductase, NADH-dependent enoyl reductase, NADH-dependent enoyl-ACP reductase, NADH-dependent enoyl-acyl carrier protein reductase, NADH-enoyl acyl carrier protein reductase, NADH-ENR, NADH-specific enoyl-ACP reductase, OsmC, PA2950, pfENR, reductase, enoyl-[acyl carrier protein], trans-enoyl-[acyl carrier protein] reductase, trans-enoyl-[acyl-carrier-protein] reductase, VEG241, vegetative protein 241, VF_0888, YP_4011

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.9 enoyl-[acyl-carrier-protein] reductase (NADH)

Purification

Purification on EC 1.3.1.9 - enoyl-[acyl-carrier-protein] reductase (NADH)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
amylose-resin-based affinity chromatography
His-bind column chromatography and Sephadex G-25 gel filtration
-
His-bind Ni2+-NTA resin column chromatography
HisPrep column chromatography
-
HisTrap column chromatography
HisTrap HP column chromatography
-
HiTrap metal chelating column chromatography, HiTrap SP FastFlow column chromatography, and amylose column chromatography
-
homogeneity
Ni-NTA affinity column chromatography
Ni-NTA agarose column chromatography
Ni-NTA agarose column chromatography, and Superdex 200 gel filtration
Ni-NTA column chromatography, Vivapure D maxi H spin column chromatography, and Blue Sepharose column chromatography
-
Ni2+ chelation chromatography
Ni2+ Hi-Trap column chromatography and Superdex 200 gel filtration
nickel-chelated His-Trap column chromatography, and Superdex 200 gel filtration
no separation from NADPH-specific enzyme
-
polyhistidine-tagged recombinant wild-type protein and mutant enzyme G95V
-
recombinant enzyme
recombinant FabI
recombinant FabI1
-
recombinant FabK
-
recombinant His-tagged InhA from Escherichia coli BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged pfENR
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged ENR from Escherichia coli BL21 by nickel affinity chromatography
-
recombinant PfENR
-
Talon metal affinity resin column chromatography, and Superdex 200 gel filtration
-
two different forms I and II
two enzymes: FabI and YgaA
using a 15Q high-resolution anion exchange column and a Hightrap butyl Sepharose column
using a HiTrap Chelating HP and an amylose column
-
using a Ni-NTA agarose column
-
using a Ni-NTA agarose, a POROS HQ20 and a Resource PHE column, and by gel filtration
using metal-chelate-affinity chromatography and gel filtration
-
usung a Ni-NTA agarose column