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1.3.1.102: 2-alkenal reductase (NADP+)

This is an abbreviated version!
For detailed information about 2-alkenal reductase (NADP+), go to the full flat file.

Word Map on EC 1.3.1.102

Reaction

an n-alkanal
+
NADP+
=
an alk-2-enal
+
NADPH
+
H+

Synonyms

2-alkenal reductase, 2-alkenal reductase (NADP+-dependent), AER, alkenal double bond reductase, allyl-ADH, allyl-ADH1, allyl-ADH2, allyl-alcohol dehydrogenase, AOR, AtAOR, chloroplastic alkenal/one oxidoreductase, double-bond reductase 1, double-bond reductase 2, NADPH-dependent alkenal/one oxidoreductase, NtDBR, NtRed-1, PaDBR1, PaDBR2

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.102 2-alkenal reductase (NADP+)

Cloned

Cloned on EC 1.3.1.102 - 2-alkenal reductase (NADP+)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His-tagged isozyme PaDBR1 in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His-tagged isozyme PaDBR2 in Escherichia coli strain BL21(DE3)
gene aor, real-time PCR. enzyme expression analysis
gene cloned into pET21b (Novagen), without a stop codon, to incorporate a C-terminal His6-tag and transformed Escherichia coli strain BL21(DE3)pLysS (Stratagene)
NtRed-1 cloned in Escherichia coli
quantitative RT-PCR enzyme expression analysis
-