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1.3.1.10: enoyl-[acyl-carrier-protein] reductase (NADPH, Si-specific)

This is an abbreviated version!
For detailed information about enoyl-[acyl-carrier-protein] reductase (NADPH, Si-specific), go to the full flat file.

Word Map on EC 1.3.1.10

Reaction

acyl-[acyl-carrier protein]
+
NADP+
=
trans-2,3-dehydroacyl-[acyl-carrier protein]
+
NADPH
+
H+

Synonyms

1-cyclohexenylcarbonyl coenzyme A reductase, 2-enoyl thioester reductase, 2-trans-enoyl-ACP reductase, acyl-ACP dehydrogenase, beta-ketoacyl reductase, enoyl acyl-carrier-protein reductase, enoyl reductase, enoyl thioester reductase, enoyl-ACP reductase, enoyl-ACP reductase II, enoyl-ACP reductase III, enoyl-ACP-reductase II, enoyl-acyl carrier protein reductase, enoyl-[acyl carrier protein] reductase, enoyl-[acyl-carrier-protein] reductase, ENR, Etr1p, fabG4, FabI, FabK, FabL, Fabl2, More, Mrf1p, NADPH 2-enoyl Co A reductase, reductase, enoyl-[acyl carrier protein] (reduced nicotinamide adenine dinucleotide phosphate), trans-enoyl-[acyl-carrier-protein] reductase, YgaA

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.10 enoyl-[acyl-carrier-protein] reductase (NADPH, Si-specific)

Cloned

Cloned on EC 1.3.1.10 - enoyl-[acyl-carrier-protein] reductase (NADPH, Si-specific)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
BcFabL from strain 6A5 is expressed in Escherichia coli strain BL21(DE3) as His6-tagged protein
chcA in pUC18 and pET3C
-
envM in bacteriophage P1
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in Escherichia coli JP1111 cells
-
expression in Escherichia coli
fabI in pUC118
-
fabI of strain RN4220 found by homology and cloned into pET15b for his-tagged overexpression
fabK in expression plasmid
-
fabL in pET15b for his-tagged overexpression
overexpression of wild-type enzyme and mutant Y73N in enzyme-deficient mutant strain BJ1991 mrf1DELTA, using the catalase 1 promotor
overexpression of wild-type enzyme and mutant Y79N in enzyme-deficient Saccharomyces cerevisiae mutant strain BJ1991 mrf1DELTA, using the catalase 1 promotor
-