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1.23.5.1: violaxanthin de-epoxidase

This is an abbreviated version!
For detailed information about violaxanthin de-epoxidase, go to the full flat file.

Word Map on EC 1.23.5.1

Reaction

antheraxanthin
+
L-ascorbate
=
zeaxanthin
+
L-dehydroascorbate
+
H2O

Synonyms

AhVDE, CsVDE, EC 1.10.99.3, lipocalin-like protein, NPQ1, VDE, Vio de-epoxidase, violaxanthin de-epoxidase, Vx de-epoxidase, ZmVDE1

ECTree

     1 Oxidoreductases
         1.23 Reducing C-O-C group as acceptor
             1.23.5 With a quinone or similar compound as acceptor
                1.23.5.1 violaxanthin de-epoxidase

Cloned

Cloned on EC 1.23.5.1 - violaxanthin de-epoxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a full-length violaxanthin de-epoxidase and deletion mutants of the N- and C-terminal regions are expressed in Escherichia coli and Nicotiana tabacum L. cv. Xanthi. High expression of the enzyme in Escherichia coli is achieved after adding the argU gene that encodes the Escherichia coli arginine AGA tRNA. The specific activity of the violaxanthin de-epoxidase expressed in Escherichia coli is low, possibly due to incorrect folding. The transformed tobacco exhibits a 13fold to 19fold increase in VDE specific activity, indicating correct protein folding
-
DNA and amino acid sequence determination and analysis, phylogenetic analysis, transiently expression of GFP-tagged enzyme in cucumber protoplasts, anti-sense expression of CsVDE in Arabidopsis thaliana leading to silencing of the single copy Arabidopsis thaliana gene, quantitative real-time PCR expression analysis
-
expressed in Escherichia coli Origami B cells
expressed in Lycopersicom esculentum
expressed in Nicotiana tabacum
-
expressed in Nicotiana tabacum NC89
expression in Escherichia coli
gene AhVDE, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Nicotiana tabacum, coexpression with anti-sense tobacco RNAi-NtVDE, semiquantitative and quantitative RT-PCR expression analysis
gene VDE1, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) in inclusion bodies
gene VDE1, recombinant expression of wild-type and truncated mutants in Escherichia coli strain BL21(DE3) in inclusion bodies
gene ZmVDE1, gene association analysis of of 155 inbred lines, and nucleotide diversity analysis, phylogenetic tree, modeling
-
gene ZmVDE1, gene association analysis of of 155 inbred lines, and nucleotide diversity analysis, phylogenetic tree, modeling, determination of full-length sequence including the promoter, 5'-UTR, exons, 3'-UTR and most of the introns from 89 inbred lines
recombinant overexpression of tagged wild-type and mutant enzymes in Escherichia coli strain Origami B
the CsVDE promoter is green tissue-specific, recombinant expression in Arabidopsis thaliana ecotype Col-0 via transformation by Agrobacterium tumefaciens strain EHA 105, the GUS signal is mainly localized to the cotyledons and the upper part of the primary roots of etiolated seedlings, a 334 bp fragment is sufficient to drive the expression of GUS to the same extent as the longest 1983 bp one in green tissue-specific manner, quantitative expression analysis. The CsVDE promoter contains multiple elements in the regulation of gene expression