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1.23.1.1: (+)-pinoresinol reductase

This is an abbreviated version!
For detailed information about (+)-pinoresinol reductase, go to the full flat file.

Word Map on EC 1.23.1.1

Reaction

(+)-lariciresinol
+
NADP+
=
(+)-pinoresinol
+
NADPH
+
H+

Synonyms

(+)-pinoresinol reductase, (+)-pinoresinol-(+)-lariciresinol reductase, (+)-pinoresinol/(+)-lariciresinol reductase, (+)pinoresinol-(+)lariciresinol reductase, bi-functional pinoresinol/lariciresinol reductase, bifunctional pinoresinol-lariciresinol reductase 2, LuPLR2, More, pinoresinol reductase, pinoresinol reductase 2, pinoresinol-lariciresinol reductase, pinoresinol-lariciresinol reductase-2, pinoresinol/lariciresinol reductase, PinZ, PLR, PLR-La1, PLR-Lc1, PLR-Lu2, PLR-Tp2, PLR1, PLR2, PrR1, PrR2, RR-pinoresinol-RR-lariciresinol reductase

ECTree

     1 Oxidoreductases
         1.23 Reducing C-O-C group as acceptor
             1.23.1 With NADH or NADPH as donor
                1.23.1.1 (+)-pinoresinol reductase

Purification

Purification on EC 1.23.1.1 - (+)-pinoresinol reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation, Affinity Blue Gel filtration, phenyl Sepharose column chromatography, hydroxyapatite column chromatography, 2’,5’ ADP-Sepharose column chromatography, Affinity-Yellow gel filtration, and Superose 12 gel filtration
-
ammonium sulfate precipitation and Affi-Gel Blue Gel column chromatography
-
His-Bind resin affinity column chromatography
metal chelate chromatography
Ni-NTA column chromatography
nickel affinity column chromatography