Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.21.99.4: thyroxine 5'-deiodinase

This is an abbreviated version!
For detailed information about thyroxine 5'-deiodinase, go to the full flat file.

Word Map on EC 1.21.99.4

Reaction

3,3',5-triiodo-L-thyronine
+
Iodide
 +
acceptor
 +
H+
=
L-thyroxine
 +
reduced acceptor

Synonyms

5DI , 5DII , 5DIII , D1 5'-deiodinase, D2 5'-deiodinase, deiodinase, diiodothyronine 5'-deiodinase, Dio, DIO1, DIO2, DIOI , DIOII , DIOIII , EC 1.97.1.10, EC 3.8.1.4, ID-1, ID-2, ID-3, iodothyronine 5'-deiodinase, Iodothyronine 5'-monodeiodinase, iodothyronine 5-deiodinase, iodothyronine deiodinase 2, iodothyronine deiodinase D2, iodothyronine inner ring monodeiodinase, iodothyronine outer ring monodeiodinase, L-thyroxine iodohydrolase (reducing), thyroxine 5-deiodinase, Thyroxine deiodinase, type 1 5'-deiodinase, type 1 deiodinase, Type 1 DI , type 1 iodothyronine deiodinase, type 2 5'-deiodinase, type 2 deiodinase, Type 2 DI , type 2 iodothyronine deiodinase, type 3 deiodinase, Type 3 DI , type I iodothyronine 5'-deiodinase, type I iodothyronine deiodinase, type I-like deiodinase, type II iodothyronine deiodinase, type II-like deiodinase, Type-I 5'deiodinase , Type-II 5'deiodinase , Type-III 5'deiodinase , types 1 iodothyronine selenodeiodinase, types 2 iodothyronine selenodeiodinase, XL-15

ECTree

     1 Oxidoreductases
         1.21 Catalysing the reaction X-H + Y-H = X-Y
             1.21.99 With unknown physiological acceptors
                1.21.99.4 thyroxine 5'-deiodinase

Cloned

Cloned on EC 1.21.99.4 - thyroxine 5'-deiodinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a promoter reporter gene for a luciferase assay is constructed
different splicing variants of DOI1 are cloned into the vector pGEM-T for sequencing, 11 variants are identified
expression in COS cells
expression in Escherichia coli via phage lambda infection
-
expression in HEK-293 cells
expression in JEG-3 cells, transient transfection
-
expression of mutant enzymes, in which selenocysteine residues in the core catalytic center is replaced by cysteine, in Saccharomyces cerevisiae
into a D10 vector
-
isozyme type I, mapping on chromosome 1p32-p33, DNA sequence analysis
-
isozyme type I, mapping on chromosome 4
-
overexpression in LLC-PK1 cells
overexpression in rat astrocytes of 29 kD subunit with and without GFP-tag via adenovirus infection
-
the P135S mutant enzyme is expressed in HEK-293 cells
-
transcription in vitro, translation in Xenopus laevis oocytes
-
transfection in HEK 293 cells
translation of purified rat RNA in Xenopus laevis oocytes
-