1.21.3.7: tetrahydrocannabinolic acid synthase

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For detailed information about tetrahydrocannabinolic acid synthase, go to the full flat file.

Reaction

Synonyms

DELA1-THCA synthase, DELTA1-tetrahydrocannabinolic acid synthase, DELTA9-tetrahydrocannabinolic acid synthase, tetrahydrocannabinolic acid synthase, tetrahydrocannabinolic acid-synthase, tetrahydrocannabinolic-acid synthase, THCA synthase, THCA-synthase, THCAS

ECTree

     1 Oxidoreductases

         1.21 Catalysing the reaction X-H + Y-H = X-Y

             1.21.3 With oxygen as acceptor

                1.21.3.7 tetrahydrocannabinolic acid synthase

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Results	in table
1	Activating Compound
3	AA Sequence
11	Cloned(Commentary)
8	Cofactor
2	Crystallization (Commentary)
1	Expression
2	General Information
3	Inhibitors
4	kcat/KM [mM/s]
7	KM Value [mM]
1	Localization
2	Metals/Ions
11	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
18	Organism
4	Pathway
1	pH Optimum
1	pI Value
5	Posttranslational Modification
10	Protein Variants
7	Purification (Commentary)
1	Reaction
1	Reaction Type
18	Reference
9	Source Tissue
2	Specific Activity [micromol/min/mg]
18	Substrates and Products (Substrate)
16	Subunits
37	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]
5	Turnover Number [1/s]

Expression

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Expression on EC 1.21.3.7 - tetrahydrocannabinolic acid synthase

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EXPRESSION

ORGANISM

UNIPROT

LITERATURE

the secreted enzyme activity in Pichia pastoris cultures is increased to 472% in the presence of 0.5% (w/v) casamino acids, 277% in the presence of proteinase inhibitors (10 mg/l 4-(2-aminoethyl) benzensulfonyl fluoride, 1 mg/l pepstatin A, and 1 mg/l E-64), 226% in the presence of 10 mg/ml FAD, 248% in the presence of 10 mg/ml FMN, 323% in the presence of 10 mg/ml riboflavin, and 298% in the presence of 1% (v/v) methanol

Cannabis sativa

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718732