1.21.3.3: reticuline oxidase

This is an abbreviated version!
For detailed information about reticuline oxidase, go to the full flat file.

Word Map on EC 1.21.3.3

1.21.3.3

poppy

sanguinarine

s-reticuline

s-scoulerine

papaver

benzylisoquinoline

somniferum

opium

benzophenanthridine

codeinone

cyp80b1

eschscholtzia

papaveraceae

vanillyl

flavinylated

3\'-hydroxylase

s-norcoclaurine

synthesis

medicine

Reaction

Synonyms

(S)-reticuline oxidase, BBE, BBE-like 13, BBE-like 15, BBE-like 28, BBE1, BBL, berberine bridge enzyme, berberine bridge enzyme-like, berberine bridge enzyme-like 28, berberine bridge-forming enzyme, berberine-bridge-forming enzyme, EC 1.5.3.9, flavin-dependent oxidase, monolignol oxidoreductase, reticuline oxidase, tetrahydroprotoberberine synthase

ECTree

1 Oxidoreductases

1.21 Catalysing the reaction X-H + Y-H = X-Y

1.21.3 With oxygen as acceptor

1.21.3.3 reticuline oxidase

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Results	in table
1	Activating Compound
48	AA Sequence
3	Application
1	CAS Registry Number
16	Cloned(Commentary)
12	Cofactor
6	Crystallization (Commentary)
1	Expression
2	General Information
13	Inhibitors
2	kcat/KM [mM/s]
7	KM Value [mM]
6	Localization
4	Molecular Weight [Da]
24	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
36	Organism
11	Pathway
13	PDB ID
5	pH Optimum
3	pH Range
2	Posttranslational Modification
14	Protein Variants
6	Purification (Commentary)
1	Reaction
7	Reaction Type
35	Reference
20	Source Tissue
4	Storage Stability
70	Substrates and Products (Substrate)
2	Subunits
40	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
14	Turnover Number [1/s]

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Cloned on EC 1.21.3.3 - reticuline oxidase

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Eschscholzia californica

P30986

726649

canadine-producing Saccharomyces cerevisiae strain harbors expression cassettes for seven heterologous enzymes: Papaper somniferum norcoclaurine 6-O-methyltransferase (Ps6OMT), Papaver somniferum 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase 2 (Ps4'OMT), Papapver somniferum coclaurine N-methyltransferase (PsCNMT), Papaver somniferum berberine bridge enzyme (PsBBE), Thalictrum flavum scoulerine 9-O-methyltransferase (TfS9OMT), Thalictrum flavum canadine synthase (TfCAS), and Arabidopsis thaliana cytochrome P450 reductase 1 (CPR). The expression cassettes for the methyltransferases Ps6OMT, PsCNMT, and Ps4'OMT and the cytochrome P450 reductase CPR were chromosomally integrated, TfS9OMT and TfCAS are expressed from a high-copy plasmid, and PsBBE is expressed from a second high-copy plasmid

Papaver somniferum

P93479

743234

expressed in Escherichia coli BL21

Papaver somniferum

657166

expressed in Komagataella pastoris

2 entries

expressed in Pichia pastoris strain GS115

Eschscholzia californica

746447

expressed in Spodoptera frugiperda (Sf9)

Eschscholzia californica

657166

expressed in Spodoptera frugiperda Sf9 cells

Eschscholzia californica

393870, 393871

expression in Pichia pastoris

2 entries

expression in Pichia pastoris strain KM71H

Eschscholzia californica

687593

for co-overexpression with disulfide isomerase in Pichia pastoris cells, for cloning of wild-type BBE, the mutant H104A and mutant H104A-C166A the vector pPICZalpha is used

Eschscholzia californica

P30986

698918

for expression in Pichia pastoris cells

Eschscholzia californica

P30986

700673

sense and antisense constructs expressed in root tissue

2 entries